Strain Fitness in Variovorax sp. SCN45 around GFF1189

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1187 and GFF1188 overlap by 4 nucleotidesGFF1188 and GFF1189 are separated by 21 nucleotidesGFF1189 and GFF1190 overlap by 4 nucleotides GFF1187 - Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3), at 504,788 to 505,729 GFF1187 GFF1188 - Thiazole synthase (EC 2.8.1.10), at 505,726 to 506,541 GFF1188 GFF1189 - Sulfur carrier protein ThiS, at 506,563 to 506,760 GFF1189 GFF1190 - Glycine oxidase ThiO (EC 1.4.3.19), at 506,757 to 507,848 GFF1190 Position (kb) 506 507Strain fitness (log2 ratio) -1 0 1at 505.602 kb on + strand, within GFF1187at 505.602 kb on + strand, within GFF1187at 505.603 kb on - strand, within GFF1187at 506.118 kb on - strand, within GFF1188at 506.118 kb on - strand, within GFF1188at 506.118 kb on - strand, within GFF1188at 506.118 kb on - strand, within GFF1188at 506.196 kb on - strand, within GFF1188at 506.196 kb on - strand, within GFF1188at 506.196 kb on - strand, within GFF1188at 506.628 kb on - strand, within GFF1189at 506.998 kb on + strand, within GFF1190at 506.999 kb on - strand, within GFF1190at 506.999 kb on - strand, within GFF1190at 506.999 kb on - strand, within GFF1190at 506.999 kb on - strand, within GFF1190at 507.109 kb on + strand, within GFF1190at 507.109 kb on + strand, within GFF1190at 507.110 kb on - strand, within GFF1190

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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505,602 + GFF1187 0.86 +0.8
505,602 + GFF1187 0.86 +0.3
505,603 - GFF1187 0.87 +0.1
506,118 - GFF1188 0.48 +0.2
506,118 - GFF1188 0.48 +1.3
506,118 - GFF1188 0.48 -0.1
506,118 - GFF1188 0.48 -0.6
506,196 - GFF1188 0.58 +0.2
506,196 - GFF1188 0.58 +0.3
506,196 - GFF1188 0.58 -0.7
506,628 - GFF1189 0.33 -0.9
506,998 + GFF1190 0.22 -1.4
506,999 - GFF1190 0.22 +0.3
506,999 - GFF1190 0.22 -0.1
506,999 - GFF1190 0.22 +0.4
506,999 - GFF1190 0.22 -0.3
507,109 + GFF1190 0.32 -1.1
507,109 + GFF1190 0.32 +0.9
507,110 - GFF1190 0.32 +1.2

Or see this region's nucleotide sequence