Strain Fitness in Variovorax sp. SCN45 around GFF1151

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1150 and GFF1151 overlap by 4 nucleotidesGFF1151 and GFF1152 are separated by 47 nucleotidesGFF1152 and GFF1153 are separated by 31 nucleotides GFF1150 - NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase, at 467,475 to 469,172 GFF1150 GFF1151 - Nitrogen regulatory protein P-II, at 469,169 to 469,507 GFF1151 GFF1152 - Lysine decarboxylase family, at 469,555 to 470,145 GFF1152 GFF1153 - Diacylglycerol kinase (EC 2.7.1.107), at 470,177 to 470,584 GFF1153 Position (kb) 469 470Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 468.339 kb on + strand, within GFF1150at 468.339 kb on + strand, within GFF1150at 468.340 kb on - strand, within GFF1150at 468.340 kb on - strand, within GFF1150at 468.340 kb on - strand, within GFF1150at 468.340 kb on - strand, within GFF1150at 468.340 kb on - strand, within GFF1150at 468.368 kb on + strand, within GFF1150at 468.472 kb on - strand, within GFF1150at 468.472 kb on - strand, within GFF1150at 468.472 kb on - strand, within GFF1150at 468.531 kb on + strand, within GFF1150at 468.726 kb on + strand, within GFF1150at 468.726 kb on + strand, within GFF1150at 468.738 kb on + strand, within GFF1150at 468.739 kb on - strand, within GFF1150at 468.739 kb on - strand, within GFF1150at 468.930 kb on + strand, within GFF1150at 468.931 kb on - strand, within GFF1150at 468.960 kb on + strand, within GFF1150at 468.960 kb on + strand, within GFF1150at 468.960 kb on + strand, within GFF1150at 468.961 kb on - strand, within GFF1150at 468.961 kb on - strand, within GFF1150at 468.961 kb on - strand, within GFF1150at 468.961 kb on - strand, within GFF1150at 468.961 kb on - strand, within GFF1150at 468.961 kb on - strand, within GFF1150at 468.961 kb on - strand, within GFF1150at 468.961 kb on - strand, within GFF1150at 468.961 kb on - strand, within GFF1150at 468.961 kb on - strand, within GFF1150at 469.005 kb on + strandat 469.056 kb on + strandat 469.056 kb on + strandat 469.056 kb on + strandat 469.056 kb on + strandat 469.056 kb on + strandat 469.056 kb on + strandat 469.056 kb on + strandat 469.057 kb on - strandat 469.057 kb on - strandat 469.057 kb on - strandat 469.057 kb on - strandat 469.057 kb on - strandat 469.127 kb on + strandat 469.128 kb on - strandat 469.128 kb on - strandat 469.128 kb on - strandat 469.128 kb on - strandat 469.305 kb on - strand, within GFF1151at 469.319 kb on + strand, within GFF1151at 469.319 kb on + strand, within GFF1151at 469.319 kb on + strand, within GFF1151at 469.319 kb on + strand, within GFF1151at 469.319 kb on + strand, within GFF1151at 469.319 kb on + strand, within GFF1151at 469.319 kb on + strand, within GFF1151at 469.320 kb on - strand, within GFF1151at 469.320 kb on - strand, within GFF1151at 469.320 kb on - strand, within GFF1151at 469.320 kb on - strand, within GFF1151at 469.402 kb on + strand, within GFF1151at 469.402 kb on + strand, within GFF1151at 469.402 kb on + strand, within GFF1151at 469.721 kb on + strand, within GFF1152at 469.721 kb on + strand, within GFF1152at 469.721 kb on + strand, within GFF1152at 469.721 kb on + strand, within GFF1152at 469.721 kb on + strand, within GFF1152at 469.721 kb on + strand, within GFF1152at 469.721 kb on + strand, within GFF1152at 469.721 kb on + strand, within GFF1152at 469.722 kb on - strand, within GFF1152at 469.722 kb on - strand, within GFF1152at 469.724 kb on + strand, within GFF1152at 469.725 kb on - strand, within GFF1152at 469.725 kb on - strand, within GFF1152at 470.025 kb on - strand, within GFF1152at 470.025 kb on - strand, within GFF1152at 470.025 kb on - strand, within GFF1152at 470.025 kb on - strand, within GFF1152at 470.025 kb on - strand, within GFF1152at 470.025 kb on - strand, within GFF1152at 470.025 kb on - strand, within GFF1152at 470.117 kb on + strandat 470.117 kb on + strandat 470.117 kb on + strandat 470.117 kb on + strandat 470.117 kb on + strandat 470.117 kb on + strandat 470.117 kb on + strandat 470.117 kb on + strandat 470.118 kb on - strandat 470.118 kb on - strandat 470.118 kb on - strandat 470.118 kb on - strandat 470.118 kb on - strandat 470.118 kb on - strandat 470.118 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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468,339 + GFF1150 0.51 -0.3
468,339 + GFF1150 0.51 +0.6
468,340 - GFF1150 0.51 +1.4
468,340 - GFF1150 0.51 -0.4
468,340 - GFF1150 0.51 +0.3
468,340 - GFF1150 0.51 -1.1
468,340 - GFF1150 0.51 -0.5
468,368 + GFF1150 0.53 -0.2
468,472 - GFF1150 0.59 +0.6
468,472 - GFF1150 0.59 -1.5
468,472 - GFF1150 0.59 -2.1
468,531 + GFF1150 0.62 +1.1
468,726 + GFF1150 0.74 +1.3
468,726 + GFF1150 0.74 -0.8
468,738 + GFF1150 0.74 +1.7
468,739 - GFF1150 0.74 -1.0
468,739 - GFF1150 0.74 -0.7
468,930 + GFF1150 0.86 -0.4
468,931 - GFF1150 0.86 +1.3
468,960 + GFF1150 0.87 +0.3
468,960 + GFF1150 0.87 -0.4
468,960 + GFF1150 0.87 +1.0
468,961 - GFF1150 0.88 -1.2
468,961 - GFF1150 0.88 +1.3
468,961 - GFF1150 0.88 -0.6
468,961 - GFF1150 0.88 +0.0
468,961 - GFF1150 0.88 +1.9
468,961 - GFF1150 0.88 -1.5
468,961 - GFF1150 0.88 -2.5
468,961 - GFF1150 0.88 -0.5
468,961 - GFF1150 0.88 +0.6
468,961 - GFF1150 0.88 +0.6
469,005 + -1.1
469,056 + -1.8
469,056 + +0.8
469,056 + +0.3
469,056 + +0.4
469,056 + -0.5
469,056 + -0.1
469,056 + -1.3
469,057 - -0.7
469,057 - -0.4
469,057 - -0.7
469,057 - -0.7
469,057 - +0.0
469,127 + -1.0
469,128 - +1.3
469,128 - -1.0
469,128 - +0.0
469,128 - -0.4
469,305 - GFF1151 0.40 -0.2
469,319 + GFF1151 0.44 -0.0
469,319 + GFF1151 0.44 -3.2
469,319 + GFF1151 0.44 -1.3
469,319 + GFF1151 0.44 +1.7
469,319 + GFF1151 0.44 -2.1
469,319 + GFF1151 0.44 +0.7
469,319 + GFF1151 0.44 -0.1
469,320 - GFF1151 0.45 -0.0
469,320 - GFF1151 0.45 +0.0
469,320 - GFF1151 0.45 -0.3
469,320 - GFF1151 0.45 +0.2
469,402 + GFF1151 0.69 +0.2
469,402 + GFF1151 0.69 -0.4
469,402 + GFF1151 0.69 +0.6
469,721 + GFF1152 0.28 -0.3
469,721 + GFF1152 0.28 -0.3
469,721 + GFF1152 0.28 -1.3
469,721 + GFF1152 0.28 -0.4
469,721 + GFF1152 0.28 -0.1
469,721 + GFF1152 0.28 -0.1
469,721 + GFF1152 0.28 -1.0
469,721 + GFF1152 0.28 +0.2
469,722 - GFF1152 0.28 +0.3
469,722 - GFF1152 0.28 -1.1
469,724 + GFF1152 0.29 +0.3
469,725 - GFF1152 0.29 +0.6
469,725 - GFF1152 0.29 -0.2
470,025 - GFF1152 0.80 +0.7
470,025 - GFF1152 0.80 -0.5
470,025 - GFF1152 0.80 -0.1
470,025 - GFF1152 0.80 -0.4
470,025 - GFF1152 0.80 +0.2
470,025 - GFF1152 0.80 +0.7
470,025 - GFF1152 0.80 -0.3
470,117 + +0.4
470,117 + +1.3
470,117 + +1.3
470,117 + -0.5
470,117 + +0.1
470,117 + -0.2
470,117 + -0.7
470,117 + +0.0
470,118 - +1.6
470,118 - -1.7
470,118 - -0.6
470,118 - +0.0
470,118 - +0.6
470,118 - -0.5
470,118 - +0.1

Or see this region's nucleotide sequence