Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1068 and GFF1069 overlap by 4 nucleotides GFF1069 and GFF1070 are separated by 63 nucleotides GFF1070 and GFF1071 are separated by 58 nucleotides GFF1071 and GFF1072 are separated by 70 nucleotides
GFF1068 - Beta-ureidopropionase (EC 3.5.1.6), at 381,114 to 382,403
GFF1068
GFF1069 - hypothetical protein, at 382,400 to 383,335
GFF1069
GFF1070 - no description, at 383,399 to 383,665
GFF1070
GFF1071 - no description, at 383,724 to 384,020
GFF1071
GFF1072 - no description, at 384,091 to 384,279
GFF1072
Position (kb) 382
383
384 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 381.436 kb on + strand, within GFF1068 at 381.715 kb on - strand, within GFF1068 at 381.728 kb on + strand, within GFF1068 at 381.859 kb on - strand, within GFF1068 at 382.371 kb on + strand at 382.644 kb on - strand, within GFF1069 at 382.709 kb on + strand, within GFF1069 at 382.709 kb on + strand, within GFF1069 at 382.710 kb on - strand, within GFF1069 at 382.710 kb on - strand, within GFF1069 at 383.107 kb on + strand, within GFF1069 at 383.108 kb on - strand, within GFF1069 at 383.350 kb on + strand at 383.350 kb on + strand at 383.351 kb on - strand at 383.413 kb on + strand at 383.413 kb on + strand at 383.413 kb on + strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.414 kb on - strand at 383.419 kb on + strand at 383.419 kb on + strand at 383.419 kb on + strand at 383.419 kb on + strand at 383.419 kb on + strand at 383.420 kb on - strand at 383.420 kb on - strand at 383.420 kb on - strand at 383.420 kb on - strand at 383.457 kb on - strand, within GFF1070 at 383.457 kb on - strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.537 kb on + strand, within GFF1070 at 383.538 kb on - strand, within GFF1070 at 383.561 kb on + strand, within GFF1070 at 383.562 kb on - strand, within GFF1070 at 383.562 kb on - strand, within GFF1070 at 383.562 kb on - strand, within GFF1070 at 383.562 kb on - strand, within GFF1070 at 383.685 kb on + strand at 383.685 kb on + strand at 383.685 kb on + strand at 383.685 kb on + strand at 383.685 kb on + strand at 383.685 kb on + strand at 383.685 kb on + strand at 383.686 kb on - strand at 383.686 kb on - strand at 383.686 kb on - strand at 383.760 kb on + strand, within GFF1071 at 383.760 kb on + strand, within GFF1071 at 383.760 kb on + strand, within GFF1071 at 383.760 kb on + strand, within GFF1071 at 383.761 kb on - strand, within GFF1071 at 383.761 kb on - strand, within GFF1071 at 383.857 kb on - strand, within GFF1071 at 383.857 kb on - strand, within GFF1071 at 383.916 kb on - strand, within GFF1071 at 383.937 kb on + strand, within GFF1071 at 383.937 kb on + strand, within GFF1071 at 383.937 kb on + strand, within GFF1071 at 383.937 kb on + strand, within GFF1071 at 383.937 kb on + strand, within GFF1071 at 383.938 kb on - strand, within GFF1071 at 383.938 kb on - strand, within GFF1071 at 384.056 kb on + strand at 384.056 kb on + strand at 384.056 kb on + strand at 384.056 kb on + strand at 384.057 kb on - strand at 384.057 kb on - strand at 384.057 kb on - strand at 384.057 kb on - strand at 384.057 kb on - strand at 384.102 kb on + strand at 384.217 kb on + strand, within GFF1072 at 384.218 kb on - strand, within GFF1072 at 384.218 kb on - strand, within GFF1072 at 384.265 kb on + strand at 384.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 381,436 + GFF1068 0.25 -0.1 381,715 - GFF1068 0.47 +1.3 381,728 + GFF1068 0.48 +0.3 381,859 - GFF1068 0.58 +0.1 382,371 + -0.3 382,644 - GFF1069 0.26 -0.5 382,709 + GFF1069 0.33 +0.7 382,709 + GFF1069 0.33 -2.9 382,710 - GFF1069 0.33 -0.1 382,710 - GFF1069 0.33 -1.4 383,107 + GFF1069 0.76 -0.1 383,108 - GFF1069 0.76 -0.8 383,350 + +1.2 383,350 + -0.4 383,351 - +0.8 383,413 + -1.5 383,413 + +0.2 383,413 + -0.7 383,414 - -1.5 383,414 - -0.3 383,414 - -0.1 383,414 - +0.5 383,414 - -1.1 383,414 - +0.5 383,414 - -0.7 383,414 - +0.3 383,414 - -1.8 383,419 + -0.5 383,419 + +0.3 383,419 + +1.7 383,419 + -0.5 383,419 + +0.2 383,420 - -0.2 383,420 - -0.7 383,420 - -0.2 383,420 - -0.2 383,457 - GFF1070 0.22 -0.2 383,457 - GFF1070 0.22 -0.2 383,537 + GFF1070 0.52 -0.3 383,537 + GFF1070 0.52 -0.8 383,537 + GFF1070 0.52 -2.5 383,537 + GFF1070 0.52 +0.7 383,537 + GFF1070 0.52 -0.2 383,537 + GFF1070 0.52 -0.0 383,538 - GFF1070 0.52 -0.7 383,561 + GFF1070 0.61 -0.1 383,562 - GFF1070 0.61 -0.6 383,562 - GFF1070 0.61 -0.5 383,562 - GFF1070 0.61 -0.5 383,562 - GFF1070 0.61 -0.6 383,685 + -0.1 383,685 + -0.6 383,685 + -0.2 383,685 + +0.1 383,685 + -0.0 383,685 + +1.3 383,685 + -0.2 383,686 - +0.0 383,686 - +0.2 383,686 - -0.0 383,760 + GFF1071 0.12 -1.2 383,760 + GFF1071 0.12 -1.5 383,760 + GFF1071 0.12 -0.0 383,760 + GFF1071 0.12 -1.0 383,761 - GFF1071 0.12 +0.2 383,761 - GFF1071 0.12 -0.2 383,857 - GFF1071 0.45 -0.2 383,857 - GFF1071 0.45 +0.3 383,916 - GFF1071 0.65 +0.3 383,937 + GFF1071 0.72 +0.1 383,937 + GFF1071 0.72 +0.1 383,937 + GFF1071 0.72 -0.4 383,937 + GFF1071 0.72 -0.4 383,937 + GFF1071 0.72 -0.1 383,938 - GFF1071 0.72 +0.7 383,938 - GFF1071 0.72 +0.1 384,056 + -0.2 384,056 + -0.8 384,056 + -0.5 384,056 + -0.2 384,057 - +0.5 384,057 - -0.2 384,057 - +0.2 384,057 - -0.8 384,057 - +0.1 384,102 + -0.1 384,217 + GFF1072 0.67 -1.1 384,218 - GFF1072 0.67 -1.5 384,218 - GFF1072 0.67 -0.0 384,265 + +0.8 384,266 - -0.6
Or see this region's nucleotide sequence