Strain Fitness in Variovorax sp. SCN45 around GFF105

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF104 and GFF105 are separated by 23 nucleotidesGFF105 and GFF106 overlap by 4 nucleotides GFF104 - General secretion pathway protein D, at 130,561 to 133,152 GFF104 GFF105 - hypothetical protein, at 133,176 to 133,748 GFF105 GFF106 - no description, at 133,745 to 134,890 GFF106 Position (kb) 133 134Strain fitness (log2 ratio) -1 0 1at 132.323 kb on + strand, within GFF104at 132.324 kb on - strand, within GFF104at 132.620 kb on + strand, within GFF104at 132.621 kb on - strand, within GFF104at 132.621 kb on - strand, within GFF104at 132.621 kb on - strand, within GFF104at 132.621 kb on - strand, within GFF104at 132.621 kb on - strand, within GFF104at 132.621 kb on - strand, within GFF104at 132.621 kb on - strand, within GFF104at 132.621 kb on - strand, within GFF104at 132.722 kb on + strand, within GFF104at 133.192 kb on + strandat 133.192 kb on + strandat 133.193 kb on - strandat 133.784 kb on - strandat 133.930 kb on - strand, within GFF106at 134.087 kb on + strand, within GFF106at 134.088 kb on - strand, within GFF106at 134.166 kb on + strand, within GFF106at 134.166 kb on + strand, within GFF106at 134.167 kb on - strand, within GFF106at 134.338 kb on + strand, within GFF106at 134.440 kb on + strand, within GFF106at 134.440 kb on + strand, within GFF106at 134.440 kb on + strand, within GFF106at 134.723 kb on + strand, within GFF106at 134.723 kb on + strand, within GFF106at 134.723 kb on + strand, within GFF106at 134.723 kb on + strand, within GFF106

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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132,323 + GFF104 0.68 +0.2
132,324 - GFF104 0.68 -1.3
132,620 + GFF104 0.79 -0.6
132,621 - GFF104 0.79 -0.0
132,621 - GFF104 0.79 -0.6
132,621 - GFF104 0.79 -0.2
132,621 - GFF104 0.79 -0.6
132,621 - GFF104 0.79 -0.8
132,621 - GFF104 0.79 -1.5
132,621 - GFF104 0.79 +0.2
132,621 - GFF104 0.79 +0.2
132,722 + GFF104 0.83 -0.0
133,192 + -0.4
133,192 + +0.1
133,193 - -0.8
133,784 - -0.0
133,930 - GFF106 0.16 -0.4
134,087 + GFF106 0.30 +0.7
134,088 - GFF106 0.30 -0.1
134,166 + GFF106 0.37 -1.2
134,166 + GFF106 0.37 -0.4
134,167 - GFF106 0.37 -0.4
134,338 + GFF106 0.52 -0.1
134,440 + GFF106 0.61 -0.8
134,440 + GFF106 0.61 -0.7
134,440 + GFF106 0.61 +1.6
134,723 + GFF106 0.85 +1.6
134,723 + GFF106 0.85 +1.2
134,723 + GFF106 0.85 -0.4
134,723 + GFF106 0.85 +0.1

Or see this region's nucleotide sequence