Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1003 and GFF1004 are separated by 16 nucleotides GFF1004 and GFF1005 are separated by 44 nucleotides GFF1005 and GFF1006 are separated by 23 nucleotides
GFF1003 - HflC protein, at 322,900 to 323,799
GFF1003
GFF1004 - Putative inner membrane protein YjeT (clustered with HflC), at 323,816 to 324,004
GFF1004
GFF1005 - ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17), at 324,049 to 325,209
GFF1005
GFF1006 - Adenylosuccinate synthetase (EC 6.3.4.4), at 325,233 to 326,564
GFF1006
Position (kb)
324
325
326 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 323.080 kb on + strand, within GFF1003 at 323.080 kb on + strand, within GFF1003 at 323.080 kb on + strand, within GFF1003 at 323.080 kb on + strand, within GFF1003 at 323.080 kb on + strand, within GFF1003 at 323.080 kb on + strand, within GFF1003 at 323.080 kb on + strand, within GFF1003 at 323.081 kb on - strand, within GFF1003 at 323.081 kb on - strand, within GFF1003 at 323.081 kb on - strand, within GFF1003 at 323.081 kb on - strand, within GFF1003 at 323.081 kb on - strand, within GFF1003 at 323.081 kb on - strand, within GFF1003 at 323.081 kb on - strand, within GFF1003 at 323.081 kb on - strand, within GFF1003 at 323.081 kb on - strand, within GFF1003 at 323.167 kb on + strand, within GFF1003 at 323.167 kb on + strand, within GFF1003 at 323.167 kb on + strand, within GFF1003 at 323.167 kb on + strand, within GFF1003 at 323.167 kb on + strand, within GFF1003 at 323.167 kb on + strand, within GFF1003 at 323.167 kb on + strand, within GFF1003 at 323.168 kb on - strand, within GFF1003 at 323.168 kb on - strand, within GFF1003 at 323.200 kb on + strand, within GFF1003 at 323.200 kb on + strand, within GFF1003 at 323.200 kb on + strand, within GFF1003 at 323.200 kb on + strand, within GFF1003 at 323.200 kb on + strand, within GFF1003 at 323.200 kb on + strand, within GFF1003 at 323.200 kb on + strand, within GFF1003 at 323.200 kb on + strand, within GFF1003 at 323.200 kb on + strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.201 kb on - strand, within GFF1003 at 323.425 kb on + strand, within GFF1003 at 323.425 kb on + strand, within GFF1003 at 323.425 kb on + strand, within GFF1003 at 323.425 kb on + strand, within GFF1003 at 323.425 kb on + strand, within GFF1003 at 323.426 kb on - strand, within GFF1003 at 323.426 kb on - strand, within GFF1003 at 323.426 kb on - strand, within GFF1003 at 323.426 kb on - strand, within GFF1003 at 323.452 kb on + strand, within GFF1003 at 323.453 kb on - strand, within GFF1003 at 323.453 kb on - strand, within GFF1003 at 323.453 kb on - strand, within GFF1003 at 323.576 kb on - strand, within GFF1003 at 323.629 kb on + strand, within GFF1003 at 323.630 kb on - strand, within GFF1003 at 323.630 kb on - strand, within GFF1003 at 323.630 kb on - strand, within GFF1003 at 323.671 kb on + strand, within GFF1003 at 323.672 kb on - strand, within GFF1003 at 323.672 kb on - strand, within GFF1003 at 323.672 kb on - strand, within GFF1003 at 323.689 kb on + strand, within GFF1003 at 323.689 kb on + strand, within GFF1003 at 323.689 kb on + strand, within GFF1003 at 323.689 kb on + strand, within GFF1003 at 323.689 kb on + strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.690 kb on - strand, within GFF1003 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.962 kb on + strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 323.963 kb on - strand, within GFF1004 at 324.016 kb on + strand at 324.017 kb on - strand at 324.017 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 323,080 + GFF1003 0.20 -0.3 323,080 + GFF1003 0.20 +1.4 323,080 + GFF1003 0.20 -0.2 323,080 + GFF1003 0.20 +0.6 323,080 + GFF1003 0.20 -0.6 323,080 + GFF1003 0.20 +0.4 323,080 + GFF1003 0.20 -1.5 323,081 - GFF1003 0.20 -0.5 323,081 - GFF1003 0.20 -0.7 323,081 - GFF1003 0.20 -0.0 323,081 - GFF1003 0.20 -1.3 323,081 - GFF1003 0.20 -0.6 323,081 - GFF1003 0.20 -1.8 323,081 - GFF1003 0.20 +2.2 323,081 - GFF1003 0.20 -1.3 323,081 - GFF1003 0.20 -0.5 323,167 + GFF1003 0.30 -0.5 323,167 + GFF1003 0.30 -0.7 323,167 + GFF1003 0.30 -1.3 323,167 + GFF1003 0.30 -0.6 323,167 + GFF1003 0.30 -0.4 323,167 + GFF1003 0.30 +0.6 323,167 + GFF1003 0.30 -2.0 323,168 - GFF1003 0.30 -3.0 323,168 - GFF1003 0.30 -2.2 323,200 + GFF1003 0.33 +0.1 323,200 + GFF1003 0.33 -0.3 323,200 + GFF1003 0.33 -0.2 323,200 + GFF1003 0.33 +0.2 323,200 + GFF1003 0.33 +0.2 323,200 + GFF1003 0.33 +0.7 323,200 + GFF1003 0.33 +0.1 323,200 + GFF1003 0.33 +0.2 323,200 + GFF1003 0.33 -0.7 323,201 - GFF1003 0.33 -0.6 323,201 - GFF1003 0.33 -1.3 323,201 - GFF1003 0.33 -0.6 323,201 - GFF1003 0.33 +0.6 323,201 - GFF1003 0.33 -1.0 323,201 - GFF1003 0.33 +0.1 323,201 - GFF1003 0.33 -1.2 323,201 - GFF1003 0.33 +0.6 323,201 - GFF1003 0.33 -0.5 323,201 - GFF1003 0.33 -1.0 323,201 - GFF1003 0.33 -1.5 323,425 + GFF1003 0.58 -0.4 323,425 + GFF1003 0.58 +0.4 323,425 + GFF1003 0.58 -0.4 323,425 + GFF1003 0.58 -0.8 323,425 + GFF1003 0.58 +0.4 323,426 - GFF1003 0.58 +0.1 323,426 - GFF1003 0.58 +0.0 323,426 - GFF1003 0.58 -1.0 323,426 - GFF1003 0.58 -0.6 323,452 + GFF1003 0.61 -0.1 323,453 - GFF1003 0.61 -0.7 323,453 - GFF1003 0.61 -1.6 323,453 - GFF1003 0.61 -1.6 323,576 - GFF1003 0.75 -1.0 323,629 + GFF1003 0.81 -0.3 323,630 - GFF1003 0.81 -0.4 323,630 - GFF1003 0.81 -0.7 323,630 - GFF1003 0.81 -0.2 323,671 + GFF1003 0.86 -0.3 323,672 - GFF1003 0.86 -0.5 323,672 - GFF1003 0.86 +0.1 323,672 - GFF1003 0.86 +0.6 323,689 + GFF1003 0.88 -1.0 323,689 + GFF1003 0.88 -1.5 323,689 + GFF1003 0.88 -0.6 323,689 + GFF1003 0.88 -0.5 323,689 + GFF1003 0.88 -1.2 323,690 - GFF1003 0.88 -0.6 323,690 - GFF1003 0.88 -0.7 323,690 - GFF1003 0.88 -1.0 323,690 - GFF1003 0.88 -0.7 323,690 - GFF1003 0.88 +0.4 323,690 - GFF1003 0.88 -0.4 323,690 - GFF1003 0.88 +0.4 323,690 - GFF1003 0.88 +0.6 323,690 - GFF1003 0.88 -0.8 323,690 - GFF1003 0.88 +0.2 323,690 - GFF1003 0.88 +0.8 323,690 - GFF1003 0.88 +0.0 323,690 - GFF1003 0.88 -0.6 323,962 + GFF1004 0.77 +0.1 323,962 + GFF1004 0.77 -0.2 323,962 + GFF1004 0.77 +0.4 323,962 + GFF1004 0.77 -1.6 323,962 + GFF1004 0.77 -1.8 323,962 + GFF1004 0.77 -0.6 323,962 + GFF1004 0.77 +0.1 323,962 + GFF1004 0.77 +0.4 323,962 + GFF1004 0.77 +0.4 323,962 + GFF1004 0.77 -0.9 323,962 + GFF1004 0.77 -0.5 323,962 + GFF1004 0.77 -0.6 323,962 + GFF1004 0.77 -0.0 323,963 - GFF1004 0.78 -1.6 323,963 - GFF1004 0.78 -0.4 323,963 - GFF1004 0.78 -0.5 323,963 - GFF1004 0.78 -0.9 323,963 - GFF1004 0.78 +0.0 323,963 - GFF1004 0.78 -0.6 323,963 - GFF1004 0.78 +0.2 323,963 - GFF1004 0.78 +1.4 323,963 - GFF1004 0.78 -1.5 323,963 - GFF1004 0.78 +0.6 323,963 - GFF1004 0.78 +1.4 323,963 - GFF1004 0.78 +1.0 323,963 - GFF1004 0.78 +0.9 323,963 - GFF1004 0.78 -0.1 323,963 - GFF1004 0.78 -0.2 324,016 + +0.0 324,017 - +0.7 324,017 - -0.3
Or see this region's nucleotide sequence