Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF999 and GFF1000 are separated by 163 nucleotides GFF1000 and GFF1001 are separated by 55 nucleotides
GFF999 - GTP-binding protein EngA, at 318,457 to 319,800
GFF999
GFF1000 - RNA-binding protein Hfq, at 319,964 to 320,209
GFF1000
GFF1001 - Ribosome LSU-associated GTP-binding protein HflX, at 320,265 to 321,434
GFF1001
Position (kb) 319
320
321 Strain fitness (log2 ratio) -2
-1
0
1
2 at 319.869 kb on + strand at 319.870 kb on - strand at 319.920 kb on + strand at 319.920 kb on + strand at 319.920 kb on + strand at 319.941 kb on + strand at 319.941 kb on + strand at 319.941 kb on + strand at 319.941 kb on + strand at 320.210 kb on + strand at 320.210 kb on + strand at 320.210 kb on + strand at 320.211 kb on - strand at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.568 kb on + strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.569 kb on - strand, within GFF1001 at 320.638 kb on + strand, within GFF1001 at 320.638 kb on + strand, within GFF1001 at 320.638 kb on + strand, within GFF1001 at 320.638 kb on + strand, within GFF1001 at 320.638 kb on + strand, within GFF1001 at 320.639 kb on - strand, within GFF1001 at 320.639 kb on - strand, within GFF1001 at 320.639 kb on - strand, within GFF1001 at 320.639 kb on - strand, within GFF1001 at 320.639 kb on - strand, within GFF1001 at 320.639 kb on - strand, within GFF1001 at 320.658 kb on + strand, within GFF1001 at 320.658 kb on + strand, within GFF1001 at 320.658 kb on + strand, within GFF1001 at 320.658 kb on + strand, within GFF1001 at 320.658 kb on + strand, within GFF1001 at 320.658 kb on + strand, within GFF1001 at 320.658 kb on + strand, within GFF1001 at 320.659 kb on - strand, within GFF1001 at 320.659 kb on - strand, within GFF1001 at 320.659 kb on - strand, within GFF1001 at 320.659 kb on - strand, within GFF1001 at 320.659 kb on - strand, within GFF1001 at 320.659 kb on - strand, within GFF1001 at 320.659 kb on - strand, within GFF1001 at 320.659 kb on - strand, within GFF1001 at 320.659 kb on - strand, within GFF1001 at 320.883 kb on + strand, within GFF1001 at 320.884 kb on - strand, within GFF1001 at 320.884 kb on - strand, within GFF1001 at 320.884 kb on - strand, within GFF1001 at 320.885 kb on + strand, within GFF1001 at 320.885 kb on + strand, within GFF1001 at 320.885 kb on + strand, within GFF1001 at 320.885 kb on + strand, within GFF1001 at 320.885 kb on + strand, within GFF1001 at 320.885 kb on + strand, within GFF1001 at 320.885 kb on + strand, within GFF1001 at 320.885 kb on + strand, within GFF1001 at 320.885 kb on + strand, within GFF1001 at 320.885 kb on + strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.886 kb on - strand, within GFF1001 at 320.937 kb on + strand, within GFF1001 at 320.937 kb on + strand, within GFF1001 at 320.937 kb on + strand, within GFF1001 at 320.938 kb on - strand, within GFF1001 at 320.938 kb on - strand, within GFF1001 at 320.938 kb on - strand, within GFF1001 at 320.938 kb on - strand, within GFF1001 at 320.988 kb on + strand, within GFF1001 at 320.988 kb on + strand, within GFF1001 at 320.988 kb on + strand, within GFF1001 at 320.988 kb on + strand, within GFF1001 at 320.988 kb on + strand, within GFF1001 at 320.989 kb on - strand, within GFF1001 at 320.989 kb on - strand, within GFF1001 at 321.144 kb on + strand, within GFF1001 at 321.145 kb on - strand, within GFF1001
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 319,869 + -0.1 319,870 - +0.5 319,920 + +0.4 319,920 + -0.2 319,920 + -1.6 319,941 + -0.1 319,941 + -1.1 319,941 + -1.2 319,941 + +1.4 320,210 + -2.0 320,210 + -0.6 320,210 + +0.1 320,211 - +0.5 320,568 + GFF1001 0.26 -1.3 320,568 + GFF1001 0.26 +1.4 320,568 + GFF1001 0.26 -0.9 320,568 + GFF1001 0.26 -0.3 320,568 + GFF1001 0.26 -0.9 320,568 + GFF1001 0.26 -0.9 320,568 + GFF1001 0.26 +0.5 320,568 + GFF1001 0.26 +0.0 320,568 + GFF1001 0.26 -1.4 320,568 + GFF1001 0.26 -0.6 320,568 + GFF1001 0.26 +1.8 320,568 + GFF1001 0.26 -0.2 320,569 - GFF1001 0.26 +0.7 320,569 - GFF1001 0.26 -0.5 320,569 - GFF1001 0.26 -0.2 320,569 - GFF1001 0.26 +0.7 320,569 - GFF1001 0.26 -0.6 320,569 - GFF1001 0.26 -1.5 320,569 - GFF1001 0.26 +0.0 320,569 - GFF1001 0.26 +0.1 320,569 - GFF1001 0.26 -0.5 320,569 - GFF1001 0.26 +0.2 320,569 - GFF1001 0.26 -0.4 320,569 - GFF1001 0.26 -0.6 320,569 - GFF1001 0.26 -0.5 320,569 - GFF1001 0.26 +0.1 320,638 + GFF1001 0.32 +0.5 320,638 + GFF1001 0.32 -0.8 320,638 + GFF1001 0.32 -0.0 320,638 + GFF1001 0.32 +0.1 320,638 + GFF1001 0.32 +1.2 320,639 - GFF1001 0.32 -0.5 320,639 - GFF1001 0.32 -0.2 320,639 - GFF1001 0.32 +0.6 320,639 - GFF1001 0.32 -0.3 320,639 - GFF1001 0.32 +0.4 320,639 - GFF1001 0.32 -0.3 320,658 + GFF1001 0.34 -0.1 320,658 + GFF1001 0.34 -0.6 320,658 + GFF1001 0.34 +0.8 320,658 + GFF1001 0.34 -1.2 320,658 + GFF1001 0.34 -0.5 320,658 + GFF1001 0.34 +0.8 320,658 + GFF1001 0.34 -0.1 320,659 - GFF1001 0.34 -0.1 320,659 - GFF1001 0.34 +0.4 320,659 - GFF1001 0.34 -1.2 320,659 - GFF1001 0.34 -0.9 320,659 - GFF1001 0.34 -1.0 320,659 - GFF1001 0.34 -0.4 320,659 - GFF1001 0.34 -0.4 320,659 - GFF1001 0.34 +0.1 320,659 - GFF1001 0.34 -0.2 320,883 + GFF1001 0.53 +1.4 320,884 - GFF1001 0.53 -0.7 320,884 - GFF1001 0.53 -0.8 320,884 - GFF1001 0.53 +0.2 320,885 + GFF1001 0.53 +0.2 320,885 + GFF1001 0.53 +0.2 320,885 + GFF1001 0.53 -0.7 320,885 + GFF1001 0.53 -0.1 320,885 + GFF1001 0.53 -1.6 320,885 + GFF1001 0.53 +0.6 320,885 + GFF1001 0.53 -0.4 320,885 + GFF1001 0.53 -0.4 320,885 + GFF1001 0.53 -0.2 320,885 + GFF1001 0.53 -0.1 320,886 - GFF1001 0.53 -0.1 320,886 - GFF1001 0.53 -0.0 320,886 - GFF1001 0.53 -0.2 320,886 - GFF1001 0.53 -0.1 320,886 - GFF1001 0.53 +0.8 320,886 - GFF1001 0.53 -1.2 320,886 - GFF1001 0.53 -0.2 320,886 - GFF1001 0.53 +0.2 320,886 - GFF1001 0.53 -0.9 320,886 - GFF1001 0.53 -1.6 320,886 - GFF1001 0.53 -0.6 320,886 - GFF1001 0.53 -0.1 320,886 - GFF1001 0.53 -0.0 320,886 - GFF1001 0.53 -0.2 320,886 - GFF1001 0.53 -0.6 320,886 - GFF1001 0.53 -0.5 320,937 + GFF1001 0.57 +1.7 320,937 + GFF1001 0.57 -0.4 320,937 + GFF1001 0.57 -0.0 320,938 - GFF1001 0.58 +0.2 320,938 - GFF1001 0.58 +0.3 320,938 - GFF1001 0.58 +0.2 320,938 - GFF1001 0.58 +0.4 320,988 + GFF1001 0.62 -1.5 320,988 + GFF1001 0.62 -1.7 320,988 + GFF1001 0.62 +0.2 320,988 + GFF1001 0.62 -0.9 320,988 + GFF1001 0.62 -0.4 320,989 - GFF1001 0.62 -1.6 320,989 - GFF1001 0.62 -0.9 321,144 + GFF1001 0.75 -0.6 321,145 - GFF1001 0.75 -0.7
Or see this region's nucleotide sequence