Strain Fitness in Escherichia coli ECRC62 around BNILDI_22295

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntallC and allE are separated by 10 nucleotidesallE and glxK are separated by 128 nucleotides BNILDI_22290: allC - allantoate deiminase, at 4,479,008 to 4,480,243 allC BNILDI_22295: allE - (S)-ureidoglycine aminohydrolase, at 4,480,254 to 4,481,039 allE BNILDI_22305: glxK - glycerate 3-kinase, at 4,481,168 to 4,482,313 glxK Position (kb) 4480 4481 4482Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 4479.356 kb on - strand, within allCat 4479.404 kb on + strand, within allCat 4479.557 kb on - strand, within allCat 4479.956 kb on - strand, within allCat 4479.969 kb on + strand, within allCat 4480.001 kb on - strand, within allCat 4480.252 kb on - strandat 4480.264 kb on + strandat 4480.264 kb on + strandat 4480.265 kb on - strandat 4480.527 kb on - strand, within allEat 4480.575 kb on + strand, within allEat 4480.647 kb on + strand, within allEat 4480.647 kb on + strand, within allEat 4480.648 kb on - strand, within allEat 4480.649 kb on + strand, within allEat 4480.649 kb on + strand, within allEat 4480.650 kb on - strand, within allEat 4480.660 kb on - strand, within allEat 4480.660 kb on - strand, within allEat 4480.834 kb on - strand, within allEat 4480.932 kb on + strand, within allEat 4481.001 kb on - strandat 4481.070 kb on - strandat 4481.094 kb on - strandat 4481.195 kb on - strandat 4481.209 kb on + strandat 4481.339 kb on + strand, within glxKat 4481.369 kb on - strand, within glxKat 4481.518 kb on + strand, within glxKat 4481.596 kb on + strand, within glxKat 4481.597 kb on - strand, within glxKat 4481.615 kb on + strand, within glxKat 4481.629 kb on - strand, within glxKat 4481.744 kb on + strand, within glxKat 4481.745 kb on - strand, within glxKat 4481.816 kb on - strand, within glxKat 4481.927 kb on - strand, within glxKat 4481.929 kb on + strand, within glxKat 4481.964 kb on - strand, within glxKat 4482.001 kb on + strand, within glxK

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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4,479,356 - allC BNILDI_22290 0.28 +0.1
4,479,404 + allC BNILDI_22290 0.32 -0.7
4,479,557 - allC BNILDI_22290 0.44 +0.7
4,479,956 - allC BNILDI_22290 0.77 +1.3
4,479,969 + allC BNILDI_22290 0.78 +0.0
4,480,001 - allC BNILDI_22290 0.80 -0.5
4,480,252 - +0.9
4,480,264 + -0.8
4,480,264 + +0.3
4,480,265 - +0.1
4,480,527 - allE BNILDI_22295 0.35 +0.8
4,480,575 + allE BNILDI_22295 0.41 -1.4
4,480,647 + allE BNILDI_22295 0.50 +3.8
4,480,647 + allE BNILDI_22295 0.50 -1.7
4,480,648 - allE BNILDI_22295 0.50 -0.4
4,480,649 + allE BNILDI_22295 0.50 -0.4
4,480,649 + allE BNILDI_22295 0.50 +1.5
4,480,650 - allE BNILDI_22295 0.50 -0.6
4,480,660 - allE BNILDI_22295 0.52 +0.1
4,480,660 - allE BNILDI_22295 0.52 -0.2
4,480,834 - allE BNILDI_22295 0.74 -1.9
4,480,932 + allE BNILDI_22295 0.86 +0.3
4,481,001 - -0.1
4,481,070 - -0.7
4,481,094 - -0.6
4,481,195 - +1.3
4,481,209 + -0.0
4,481,339 + glxK BNILDI_22305 0.15 +0.4
4,481,369 - glxK BNILDI_22305 0.18 +2.4
4,481,518 + glxK BNILDI_22305 0.31 -0.0
4,481,596 + glxK BNILDI_22305 0.37 -0.1
4,481,597 - glxK BNILDI_22305 0.37 +0.0
4,481,615 + glxK BNILDI_22305 0.39 +0.9
4,481,629 - glxK BNILDI_22305 0.40 +1.2
4,481,744 + glxK BNILDI_22305 0.50 -0.1
4,481,745 - glxK BNILDI_22305 0.50 +0.5
4,481,816 - glxK BNILDI_22305 0.57 -0.2
4,481,927 - glxK BNILDI_22305 0.66 +0.3
4,481,929 + glxK BNILDI_22305 0.66 -0.3
4,481,964 - glxK BNILDI_22305 0.69 +0.4
4,482,001 + glxK BNILDI_22305 0.73 -0.5

Or see this region's nucleotide sequence