Strain Fitness in Escherichia coli ECRC62 around BNILDI_20285

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycaI and ihfB are separated by 206 nucleotidesihfB and rpsA are separated by 159 nucleotidesrpsA and cmk are separated by 110 nucleotidescmk and ycaL are separated by 172 nucleotides BNILDI_20275: ycaI - ComEC family protein, at 4,075,090 to 4,077,354 ycaI BNILDI_20280: ihfB - integration host factor subunit beta, at 4,077,561 to 4,077,845 ihfB BNILDI_20285: rpsA - 30S ribosomal protein S1, at 4,078,005 to 4,079,678 rpsA BNILDI_20290: cmk - (d)CMP kinase, at 4,079,789 to 4,080,472 cmk BNILDI_20295: ycaL - metallopeptidase YcaL, at 4,080,645 to 4,081,409 ycaL Position (kb) 4078 4079 4080Strain fitness (log2 ratio) -2 -1 0 1 2at 4077.039 kb on + strand, within ycaIat 4077.039 kb on + strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.040 kb on - strand, within ycaIat 4077.052 kb on + strand, within ycaIat 4077.053 kb on - strand, within ycaIat 4077.061 kb on - strand, within ycaIat 4077.071 kb on + strand, within ycaIat 4077.134 kb on - strandat 4077.170 kb on - strandat 4077.172 kb on + strandat 4077.173 kb on - strandat 4077.209 kb on + strandat 4077.210 kb on - strandat 4077.325 kb on + strandat 4077.326 kb on - strandat 4077.326 kb on - strandat 4077.346 kb on + strandat 4077.569 kb on - strandat 4077.605 kb on + strand, within ihfBat 4078.017 kb on - strandat 4080.485 kb on - strandat 4080.522 kb on - strandat 4080.646 kb on + strandat 4080.646 kb on + strandat 4080.647 kb on - strandat 4080.650 kb on + strandat 4080.651 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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4,077,039 + ycaI BNILDI_20275 0.86 +0.6
4,077,039 + ycaI BNILDI_20275 0.86 +1.9
4,077,040 - ycaI BNILDI_20275 0.86 +1.3
4,077,040 - ycaI BNILDI_20275 0.86 +0.9
4,077,040 - ycaI BNILDI_20275 0.86 +0.1
4,077,040 - ycaI BNILDI_20275 0.86 -0.2
4,077,040 - ycaI BNILDI_20275 0.86 +0.9
4,077,052 + ycaI BNILDI_20275 0.87 -0.5
4,077,053 - ycaI BNILDI_20275 0.87 -0.1
4,077,061 - ycaI BNILDI_20275 0.87 +0.8
4,077,071 + ycaI BNILDI_20275 0.87 +0.6
4,077,134 - +0.5
4,077,170 - +0.1
4,077,172 + -0.3
4,077,173 - -0.0
4,077,209 + +1.4
4,077,210 - -0.1
4,077,325 + +0.2
4,077,326 - +0.5
4,077,326 - +0.3
4,077,346 + +0.3
4,077,569 - +1.1
4,077,605 + ihfB BNILDI_20280 0.15 +0.9
4,078,017 - -2.5
4,080,485 - -1.8
4,080,522 - +0.4
4,080,646 + -0.2
4,080,646 + +0.9
4,080,647 - -0.1
4,080,650 + -0.1
4,080,651 - -0.2

Or see this region's nucleotide sequence