Experiment: MPec2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcF and hrpA are separated by 271 nucleotides hrpA and azoR are separated by 200 nucleotides azoR and ynbD are separated by 50 nucleotides
BNILDI_17840: ydcF - Protein YdcF, at 3,586,444 to 3,587,244
ydcF
BNILDI_17845: hrpA - ATP-dependent RNA helicase HrpA, at 3,587,516 to 3,591,418
hrpA
BNILDI_17850: azoR - FMN-dependent NADH-azoreductase, at 3,591,619 to 3,592,224
azoR
BNILDI_17855: ynbD - Uncharacterized protein YnbD, at 3,592,275 to 3,593,591
ynbD
Position (kb)
3587
3588
3589
3590
3591
3592 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3586.578 kb on + strand, within ydcF at 3586.640 kb on - strand, within ydcF at 3586.667 kb on - strand, within ydcF at 3586.714 kb on + strand, within ydcF at 3586.838 kb on - strand, within ydcF at 3587.079 kb on + strand, within ydcF at 3587.080 kb on - strand, within ydcF at 3587.194 kb on + strand at 3587.195 kb on - strand at 3587.203 kb on + strand at 3587.203 kb on + strand at 3587.204 kb on - strand at 3587.204 kb on - strand at 3587.205 kb on + strand at 3587.205 kb on + strand at 3587.205 kb on + strand at 3587.284 kb on + strand at 3587.287 kb on + strand at 3587.323 kb on + strand at 3587.324 kb on - strand at 3587.324 kb on - strand at 3587.324 kb on - strand at 3587.357 kb on - strand at 3587.376 kb on + strand at 3587.381 kb on - strand at 3587.387 kb on + strand at 3587.449 kb on + strand at 3587.455 kb on + strand at 3587.548 kb on + strand at 3587.596 kb on - strand at 3587.616 kb on - strand at 3587.841 kb on - strand at 3588.064 kb on + strand, within hrpA at 3588.083 kb on - strand, within hrpA at 3588.126 kb on + strand, within hrpA at 3588.312 kb on + strand, within hrpA at 3588.333 kb on + strand, within hrpA at 3588.364 kb on + strand, within hrpA at 3588.365 kb on - strand, within hrpA at 3588.368 kb on + strand, within hrpA at 3588.368 kb on + strand, within hrpA at 3588.369 kb on - strand, within hrpA at 3588.369 kb on - strand, within hrpA at 3588.471 kb on - strand, within hrpA at 3588.632 kb on + strand, within hrpA at 3588.698 kb on - strand, within hrpA at 3588.770 kb on - strand, within hrpA at 3588.787 kb on + strand, within hrpA at 3588.788 kb on - strand, within hrpA at 3588.791 kb on + strand, within hrpA at 3588.791 kb on + strand, within hrpA at 3589.171 kb on + strand, within hrpA at 3589.474 kb on + strand, within hrpA at 3589.487 kb on - strand, within hrpA at 3589.489 kb on - strand, within hrpA at 3589.529 kb on + strand, within hrpA at 3589.530 kb on - strand, within hrpA at 3589.627 kb on - strand, within hrpA at 3589.627 kb on - strand, within hrpA at 3589.631 kb on - strand, within hrpA at 3589.631 kb on - strand, within hrpA at 3589.631 kb on - strand, within hrpA at 3589.656 kb on + strand, within hrpA at 3590.194 kb on + strand, within hrpA at 3590.195 kb on - strand, within hrpA at 3590.308 kb on + strand, within hrpA at 3590.308 kb on + strand, within hrpA at 3590.309 kb on - strand, within hrpA at 3590.334 kb on + strand, within hrpA at 3590.335 kb on - strand, within hrpA at 3590.335 kb on - strand, within hrpA at 3590.335 kb on - strand, within hrpA at 3590.339 kb on - strand, within hrpA at 3590.480 kb on + strand, within hrpA at 3590.481 kb on - strand, within hrpA at 3590.530 kb on + strand, within hrpA at 3590.531 kb on - strand, within hrpA at 3590.658 kb on + strand, within hrpA at 3590.692 kb on + strand, within hrpA at 3590.751 kb on - strand, within hrpA at 3590.784 kb on + strand, within hrpA at 3590.784 kb on + strand, within hrpA at 3590.847 kb on + strand, within hrpA at 3590.847 kb on + strand, within hrpA at 3590.996 kb on - strand, within hrpA at 3591.056 kb on - strand at 3591.201 kb on + strand at 3591.201 kb on + strand at 3591.205 kb on + strand at 3591.419 kb on - strand at 3591.465 kb on + strand at 3591.466 kb on - strand at 3591.470 kb on - strand at 3591.471 kb on + strand at 3591.607 kb on + strand at 3591.630 kb on - strand at 3591.632 kb on + strand at 3591.633 kb on - strand at 3591.662 kb on - strand at 3591.662 kb on - strand at 3591.908 kb on - strand, within azoR at 3591.908 kb on - strand, within azoR at 3591.910 kb on - strand, within azoR at 3592.029 kb on + strand, within azoR at 3592.029 kb on + strand, within azoR at 3592.030 kb on - strand, within azoR at 3592.084 kb on + strand, within azoR at 3592.153 kb on + strand, within azoR at 3592.250 kb on + strand at 3592.277 kb on - strand at 3592.320 kb on - strand at 3592.376 kb on + strand at 3592.377 kb on - strand at 3592.392 kb on + strand at 3592.393 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MPec2 remove 3,586,578 + ydcF BNILDI_17840 0.17 -1.1 3,586,640 - ydcF BNILDI_17840 0.24 +0.9 3,586,667 - ydcF BNILDI_17840 0.28 +1.1 3,586,714 + ydcF BNILDI_17840 0.34 -0.8 3,586,838 - ydcF BNILDI_17840 0.49 -0.4 3,587,079 + ydcF BNILDI_17840 0.79 -1.9 3,587,080 - ydcF BNILDI_17840 0.79 -1.8 3,587,194 + +0.3 3,587,195 - -1.5 3,587,203 + +1.5 3,587,203 + -0.7 3,587,204 - +0.2 3,587,204 - -0.4 3,587,205 + -0.2 3,587,205 + -0.9 3,587,205 + +0.2 3,587,284 + +0.2 3,587,287 + +0.1 3,587,323 + -0.5 3,587,324 - -0.4 3,587,324 - +0.2 3,587,324 - -0.2 3,587,357 - +0.8 3,587,376 + +0.9 3,587,381 - -1.8 3,587,387 + -0.9 3,587,449 + -0.4 3,587,455 + -0.9 3,587,548 + +0.9 3,587,596 - -0.5 3,587,616 - -0.4 3,587,841 - +0.2 3,588,064 + hrpA BNILDI_17845 0.14 +0.0 3,588,083 - hrpA BNILDI_17845 0.15 +3.2 3,588,126 + hrpA BNILDI_17845 0.16 -0.1 3,588,312 + hrpA BNILDI_17845 0.20 -0.1 3,588,333 + hrpA BNILDI_17845 0.21 +0.1 3,588,364 + hrpA BNILDI_17845 0.22 +0.1 3,588,365 - hrpA BNILDI_17845 0.22 +0.6 3,588,368 + hrpA BNILDI_17845 0.22 +0.3 3,588,368 + hrpA BNILDI_17845 0.22 -0.0 3,588,369 - hrpA BNILDI_17845 0.22 -1.3 3,588,369 - hrpA BNILDI_17845 0.22 +0.8 3,588,471 - hrpA BNILDI_17845 0.24 -1.2 3,588,632 + hrpA BNILDI_17845 0.29 -1.0 3,588,698 - hrpA BNILDI_17845 0.30 -0.3 3,588,770 - hrpA BNILDI_17845 0.32 -0.2 3,588,787 + hrpA BNILDI_17845 0.33 +0.1 3,588,788 - hrpA BNILDI_17845 0.33 -0.6 3,588,791 + hrpA BNILDI_17845 0.33 -0.4 3,588,791 + hrpA BNILDI_17845 0.33 -1.2 3,589,171 + hrpA BNILDI_17845 0.42 -0.4 3,589,474 + hrpA BNILDI_17845 0.50 -1.4 3,589,487 - hrpA BNILDI_17845 0.50 +0.2 3,589,489 - hrpA BNILDI_17845 0.51 -0.5 3,589,529 + hrpA BNILDI_17845 0.52 -0.4 3,589,530 - hrpA BNILDI_17845 0.52 +0.6 3,589,627 - hrpA BNILDI_17845 0.54 -0.3 3,589,627 - hrpA BNILDI_17845 0.54 -0.0 3,589,631 - hrpA BNILDI_17845 0.54 -1.1 3,589,631 - hrpA BNILDI_17845 0.54 +0.4 3,589,631 - hrpA BNILDI_17845 0.54 -1.1 3,589,656 + hrpA BNILDI_17845 0.55 -0.1 3,590,194 + hrpA BNILDI_17845 0.69 -1.9 3,590,195 - hrpA BNILDI_17845 0.69 -1.1 3,590,308 + hrpA BNILDI_17845 0.72 -0.2 3,590,308 + hrpA BNILDI_17845 0.72 +0.6 3,590,309 - hrpA BNILDI_17845 0.72 +2.0 3,590,334 + hrpA BNILDI_17845 0.72 -1.1 3,590,335 - hrpA BNILDI_17845 0.72 +0.1 3,590,335 - hrpA BNILDI_17845 0.72 +0.2 3,590,335 - hrpA BNILDI_17845 0.72 +0.3 3,590,339 - hrpA BNILDI_17845 0.72 -0.6 3,590,480 + hrpA BNILDI_17845 0.76 -0.0 3,590,481 - hrpA BNILDI_17845 0.76 -0.5 3,590,530 + hrpA BNILDI_17845 0.77 -0.3 3,590,531 - hrpA BNILDI_17845 0.77 +0.2 3,590,658 + hrpA BNILDI_17845 0.81 +0.9 3,590,692 + hrpA BNILDI_17845 0.81 +0.6 3,590,751 - hrpA BNILDI_17845 0.83 -0.5 3,590,784 + hrpA BNILDI_17845 0.84 -0.1 3,590,784 + hrpA BNILDI_17845 0.84 -0.9 3,590,847 + hrpA BNILDI_17845 0.85 +0.5 3,590,847 + hrpA BNILDI_17845 0.85 -0.4 3,590,996 - hrpA BNILDI_17845 0.89 +0.6 3,591,056 - -0.1 3,591,201 + +0.3 3,591,201 + +0.1 3,591,205 + -0.2 3,591,419 - -0.7 3,591,465 + +1.6 3,591,466 - -0.7 3,591,470 - -0.1 3,591,471 + -1.1 3,591,607 + -0.9 3,591,630 - +0.3 3,591,632 + +0.6 3,591,633 - -0.1 3,591,662 - +0.1 3,591,662 - +2.2 3,591,908 - azoR BNILDI_17850 0.48 -1.4 3,591,908 - azoR BNILDI_17850 0.48 -1.4 3,591,910 - azoR BNILDI_17850 0.48 +0.2 3,592,029 + azoR BNILDI_17850 0.68 +0.6 3,592,029 + azoR BNILDI_17850 0.68 -2.6 3,592,030 - azoR BNILDI_17850 0.68 -0.6 3,592,084 + azoR BNILDI_17850 0.77 +0.3 3,592,153 + azoR BNILDI_17850 0.88 -0.1 3,592,250 + +0.1 3,592,277 - -0.2 3,592,320 - -0.7 3,592,376 + +1.6 3,592,377 - -1.2 3,592,392 + +0.2 3,592,393 - -0.3
Or see this region's nucleotide sequence