Strain Fitness in Escherichia coli ECRC62 around BNILDI_17045

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmlc and bioD are separated by 124 nucleotidesbioD and clcB overlap by 48 nucleotidesclcB and dmsD are separated by 194 nucleotidesdmsD and ynfH are separated by 42 nucleotides BNILDI_17035: mlc - DNA-binding transcriptional repressor Mlc, at 3,412,775 to 3,413,995 mlc BNILDI_17040: bioD - dethiobiotin synthase, at 3,414,120 to 3,414,815 bioD BNILDI_17045: clcB - voltage-gated ClC-type chloride channel ClcB, at 3,414,768 to 3,416,024 clcB BNILDI_17050: dmsD - Tat proofreading chaperone DmsD, at 3,416,219 to 3,416,833 dmsD BNILDI_17055: ynfH - Anaerobic dimethyl sulfoxide reductase chain YnfH, at 3,416,876 to 3,417,730 ynfH Position (kb) 3414 3415 3416 3417Strain fitness (log2 ratio) -2 -1 0 1 2at 3413.957 kb on + strandat 3414.089 kb on - strandat 3414.230 kb on + strand, within bioDat 3414.230 kb on + strand, within bioDat 3414.365 kb on + strand, within bioDat 3414.451 kb on + strand, within bioDat 3414.452 kb on - strand, within bioDat 3414.566 kb on + strand, within bioDat 3414.723 kb on + strand, within bioDat 3414.759 kb on + strandat 3414.769 kb on + strandat 3414.770 kb on - strandat 3414.773 kb on + strandat 3414.774 kb on - strandat 3414.800 kb on + strandat 3414.800 kb on + strandat 3414.840 kb on + strandat 3414.856 kb on - strandat 3414.867 kb on + strandat 3415.018 kb on + strand, within clcBat 3415.113 kb on - strand, within clcBat 3415.113 kb on - strand, within clcBat 3415.170 kb on + strand, within clcBat 3415.179 kb on - strand, within clcBat 3415.302 kb on + strand, within clcBat 3415.363 kb on + strand, within clcBat 3415.395 kb on + strand, within clcBat 3415.396 kb on - strand, within clcBat 3415.521 kb on + strand, within clcBat 3415.522 kb on - strand, within clcBat 3415.522 kb on - strand, within clcBat 3415.648 kb on - strand, within clcBat 3415.860 kb on + strand, within clcBat 3415.941 kb on + strandat 3416.025 kb on - strandat 3416.042 kb on - strandat 3416.042 kb on - strandat 3416.049 kb on + strandat 3416.049 kb on + strandat 3416.049 kb on + strandat 3416.050 kb on - strandat 3416.093 kb on - strandat 3416.209 kb on + strandat 3416.210 kb on - strandat 3416.917 kb on - strandat 3416.917 kb on - strandat 3416.917 kb on - strandat 3416.971 kb on + strand, within ynfH

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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3,413,957 + -0.3
3,414,089 - -0.4
3,414,230 + bioD BNILDI_17040 0.16 -0.7
3,414,230 + bioD BNILDI_17040 0.16 +1.6
3,414,365 + bioD BNILDI_17040 0.35 -0.2
3,414,451 + bioD BNILDI_17040 0.48 -0.2
3,414,452 - bioD BNILDI_17040 0.48 +0.4
3,414,566 + bioD BNILDI_17040 0.64 -0.1
3,414,723 + bioD BNILDI_17040 0.87 +0.1
3,414,759 + +1.6
3,414,769 + -0.1
3,414,770 - +0.0
3,414,773 + +0.1
3,414,774 - +0.6
3,414,800 + -0.5
3,414,800 + +2.0
3,414,840 + -1.4
3,414,856 - +1.6
3,414,867 + +2.6
3,415,018 + clcB BNILDI_17045 0.20 -0.5
3,415,113 - clcB BNILDI_17045 0.27 +0.6
3,415,113 - clcB BNILDI_17045 0.27 +0.6
3,415,170 + clcB BNILDI_17045 0.32 -0.8
3,415,179 - clcB BNILDI_17045 0.33 +1.6
3,415,302 + clcB BNILDI_17045 0.42 -0.1
3,415,363 + clcB BNILDI_17045 0.47 +1.6
3,415,395 + clcB BNILDI_17045 0.50 +1.6
3,415,396 - clcB BNILDI_17045 0.50 -0.4
3,415,521 + clcB BNILDI_17045 0.60 -0.4
3,415,522 - clcB BNILDI_17045 0.60 +0.5
3,415,522 - clcB BNILDI_17045 0.60 +0.3
3,415,648 - clcB BNILDI_17045 0.70 +0.6
3,415,860 + clcB BNILDI_17045 0.87 +0.5
3,415,941 + +0.6
3,416,025 - +0.2
3,416,042 - +0.8
3,416,042 - +1.4
3,416,049 + +0.6
3,416,049 + +0.8
3,416,049 + -2.0
3,416,050 - +1.3
3,416,093 - +0.6
3,416,209 + +0.1
3,416,210 - +0.7
3,416,917 - +0.6
3,416,917 - -0.8
3,416,917 - -0.2
3,416,971 + ynfH BNILDI_17055 0.11 -0.1

Or see this region's nucleotide sequence