Experiment: MPec2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mlc and bioD are separated by 124 nucleotides bioD and clcB overlap by 48 nucleotides clcB and dmsD are separated by 194 nucleotides dmsD and ynfH are separated by 42 nucleotides
BNILDI_17035: mlc - DNA-binding transcriptional repressor Mlc, at 3,412,775 to 3,413,995
mlc
BNILDI_17040: bioD - dethiobiotin synthase, at 3,414,120 to 3,414,815
bioD
BNILDI_17045: clcB - voltage-gated ClC-type chloride channel ClcB, at 3,414,768 to 3,416,024
clcB
BNILDI_17050: dmsD - Tat proofreading chaperone DmsD, at 3,416,219 to 3,416,833
dmsD
BNILDI_17055: ynfH - Anaerobic dimethyl sulfoxide reductase chain YnfH, at 3,416,876 to 3,417,730
ynfH
Position (kb)
3414
3415
3416
3417 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3413.957 kb on + strand at 3414.089 kb on - strand at 3414.230 kb on + strand, within bioD at 3414.230 kb on + strand, within bioD at 3414.365 kb on + strand, within bioD at 3414.451 kb on + strand, within bioD at 3414.452 kb on - strand, within bioD at 3414.566 kb on + strand, within bioD at 3414.723 kb on + strand, within bioD at 3414.759 kb on + strand at 3414.769 kb on + strand at 3414.770 kb on - strand at 3414.773 kb on + strand at 3414.774 kb on - strand at 3414.800 kb on + strand at 3414.800 kb on + strand at 3414.840 kb on + strand at 3414.856 kb on - strand at 3414.867 kb on + strand at 3415.018 kb on + strand, within clcB at 3415.113 kb on - strand, within clcB at 3415.113 kb on - strand, within clcB at 3415.170 kb on + strand, within clcB at 3415.179 kb on - strand, within clcB at 3415.302 kb on + strand, within clcB at 3415.363 kb on + strand, within clcB at 3415.395 kb on + strand, within clcB at 3415.396 kb on - strand, within clcB at 3415.521 kb on + strand, within clcB at 3415.522 kb on - strand, within clcB at 3415.522 kb on - strand, within clcB at 3415.648 kb on - strand, within clcB at 3415.860 kb on + strand, within clcB at 3415.941 kb on + strand at 3416.025 kb on - strand at 3416.042 kb on - strand at 3416.042 kb on - strand at 3416.049 kb on + strand at 3416.049 kb on + strand at 3416.049 kb on + strand at 3416.050 kb on - strand at 3416.093 kb on - strand at 3416.209 kb on + strand at 3416.210 kb on - strand at 3416.917 kb on - strand at 3416.917 kb on - strand at 3416.917 kb on - strand at 3416.971 kb on + strand, within ynfH
Per-strain Table
Position Strand Gene LocusTag Fraction MPec2 remove 3,413,957 + -0.3 3,414,089 - -0.4 3,414,230 + bioD BNILDI_17040 0.16 -0.7 3,414,230 + bioD BNILDI_17040 0.16 +1.6 3,414,365 + bioD BNILDI_17040 0.35 -0.2 3,414,451 + bioD BNILDI_17040 0.48 -0.2 3,414,452 - bioD BNILDI_17040 0.48 +0.4 3,414,566 + bioD BNILDI_17040 0.64 -0.1 3,414,723 + bioD BNILDI_17040 0.87 +0.1 3,414,759 + +1.6 3,414,769 + -0.1 3,414,770 - +0.0 3,414,773 + +0.1 3,414,774 - +0.6 3,414,800 + -0.5 3,414,800 + +2.0 3,414,840 + -1.4 3,414,856 - +1.6 3,414,867 + +2.6 3,415,018 + clcB BNILDI_17045 0.20 -0.5 3,415,113 - clcB BNILDI_17045 0.27 +0.6 3,415,113 - clcB BNILDI_17045 0.27 +0.6 3,415,170 + clcB BNILDI_17045 0.32 -0.8 3,415,179 - clcB BNILDI_17045 0.33 +1.6 3,415,302 + clcB BNILDI_17045 0.42 -0.1 3,415,363 + clcB BNILDI_17045 0.47 +1.6 3,415,395 + clcB BNILDI_17045 0.50 +1.6 3,415,396 - clcB BNILDI_17045 0.50 -0.4 3,415,521 + clcB BNILDI_17045 0.60 -0.4 3,415,522 - clcB BNILDI_17045 0.60 +0.5 3,415,522 - clcB BNILDI_17045 0.60 +0.3 3,415,648 - clcB BNILDI_17045 0.70 +0.6 3,415,860 + clcB BNILDI_17045 0.87 +0.5 3,415,941 + +0.6 3,416,025 - +0.2 3,416,042 - +0.8 3,416,042 - +1.4 3,416,049 + +0.6 3,416,049 + +0.8 3,416,049 + -2.0 3,416,050 - +1.3 3,416,093 - +0.6 3,416,209 + +0.1 3,416,210 - +0.7 3,416,917 - +0.6 3,416,917 - -0.8 3,416,917 - -0.2 3,416,971 + ynfH BNILDI_17055 0.11 -0.1
Or see this region's nucleotide sequence