Experiment: MPec2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt selD and topB are separated by 4 nucleotides topB and BNILDI_16130 are separated by 127 nucleotides BNILDI_16130 and ynjI are separated by 17 nucleotides
BNILDI_16120: selD - selenide, water dikinase SelD, at 3,234,007 to 3,235,050
selD
BNILDI_16125: topB - DNA topoisomerase III, at 3,235,055 to 3,237,016
topB
BNILDI_16130: BNILDI_16130 - Inner membrane protein, at 3,237,144 to 3,237,284
_16130
BNILDI_16135: ynjI - Inner membrane protein YnjI, at 3,237,302 to 3,238,186
ynjI
Position (kb) 3235
3236
3237
3238 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 3234.170 kb on - strand, within selD at 3234.385 kb on + strand, within selD at 3234.796 kb on + strand, within selD at 3234.801 kb on + strand, within selD at 3234.801 kb on + strand, within selD at 3234.802 kb on - strand, within selD at 3234.802 kb on - strand, within selD at 3235.126 kb on + strand at 3235.278 kb on + strand, within topB at 3235.310 kb on - strand, within topB at 3235.569 kb on - strand, within topB at 3235.579 kb on + strand, within topB at 3235.579 kb on + strand, within topB at 3235.595 kb on - strand, within topB at 3235.629 kb on + strand, within topB at 3235.630 kb on - strand, within topB at 3235.889 kb on + strand, within topB at 3235.997 kb on - strand, within topB at 3236.036 kb on + strand, within topB at 3236.145 kb on + strand, within topB at 3236.146 kb on - strand, within topB at 3236.146 kb on - strand, within topB at 3236.261 kb on + strand, within topB at 3236.262 kb on - strand, within topB at 3236.473 kb on - strand, within topB at 3236.694 kb on - strand, within topB at 3236.850 kb on + strand at 3236.850 kb on + strand at 3236.853 kb on - strand at 3237.014 kb on + strand at 3237.212 kb on + strand, within BNILDI_16130 at 3237.443 kb on + strand, within ynjI at 3237.628 kb on - strand, within ynjI at 3237.684 kb on - strand, within ynjI at 3237.737 kb on - strand, within ynjI at 3237.876 kb on + strand, within ynjI at 3237.950 kb on - strand, within ynjI
Per-strain Table
Position Strand Gene LocusTag Fraction MPec2 remove 3,234,170 - selD BNILDI_16120 0.16 +0.3 3,234,385 + selD BNILDI_16120 0.36 -0.8 3,234,796 + selD BNILDI_16120 0.76 +0.0 3,234,801 + selD BNILDI_16120 0.76 +0.2 3,234,801 + selD BNILDI_16120 0.76 -0.0 3,234,802 - selD BNILDI_16120 0.76 +0.2 3,234,802 - selD BNILDI_16120 0.76 -0.2 3,235,126 + -1.7 3,235,278 + topB BNILDI_16125 0.11 +0.0 3,235,310 - topB BNILDI_16125 0.13 -0.2 3,235,569 - topB BNILDI_16125 0.26 +1.0 3,235,579 + topB BNILDI_16125 0.27 +1.0 3,235,579 + topB BNILDI_16125 0.27 +0.2 3,235,595 - topB BNILDI_16125 0.28 +1.9 3,235,629 + topB BNILDI_16125 0.29 -0.3 3,235,630 - topB BNILDI_16125 0.29 +0.6 3,235,889 + topB BNILDI_16125 0.43 -0.1 3,235,997 - topB BNILDI_16125 0.48 -0.3 3,236,036 + topB BNILDI_16125 0.50 -1.0 3,236,145 + topB BNILDI_16125 0.56 -0.2 3,236,146 - topB BNILDI_16125 0.56 -0.2 3,236,146 - topB BNILDI_16125 0.56 +0.2 3,236,261 + topB BNILDI_16125 0.61 -1.2 3,236,262 - topB BNILDI_16125 0.62 +1.3 3,236,473 - topB BNILDI_16125 0.72 +0.6 3,236,694 - topB BNILDI_16125 0.84 +0.2 3,236,850 + +0.6 3,236,850 + +4.1 3,236,853 - +1.2 3,237,014 + +1.9 3,237,212 + BNILDI_16130 0.48 +0.6 3,237,443 + ynjI BNILDI_16135 0.16 -0.6 3,237,628 - ynjI BNILDI_16135 0.37 +0.9 3,237,684 - ynjI BNILDI_16135 0.43 -0.6 3,237,737 - ynjI BNILDI_16135 0.49 +0.0 3,237,876 + ynjI BNILDI_16135 0.65 -1.1 3,237,950 - ynjI BNILDI_16135 0.73 -0.2
Or see this region's nucleotide sequence