Experiment: MPec2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yedE and yedD are separated by 186 nucleotides yedD and amyA are separated by 33 nucleotides amyA and fliT are separated by 77 nucleotides fliT and fliS overlap by 1 nucleotides fliS and fliD are separated by 24 nucleotides
BNILDI_15155: yedE - selenium metabolism membrane protein YedE/FdhT, at 3,053,395 to 3,054,600
yedE
BNILDI_15160: yedD - lipoprotein, at 3,054,787 to 3,055,200
yedD
BNILDI_15165: amyA - alpha-amylase, at 3,055,234 to 3,056,721
amyA
BNILDI_15170: fliT - flagella biosynthesis regulatory protein FliT, at 3,056,799 to 3,057,164
fliT
BNILDI_15175: fliS - flagellar export chaperone FliS, at 3,057,164 to 3,057,574
fliS
BNILDI_15180: fliD - flagellar filament capping protein FliD, at 3,057,599 to 3,059,005
fliD
Position (kb)
3055
3056
3057 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3054.264 kb on + strand, within yedE at 3054.293 kb on - strand, within yedE at 3054.293 kb on - strand, within yedE at 3054.343 kb on - strand, within yedE at 3054.374 kb on - strand, within yedE at 3054.397 kb on - strand, within yedE at 3054.397 kb on - strand, within yedE at 3054.509 kb on + strand at 3054.516 kb on + strand at 3054.565 kb on - strand at 3054.606 kb on + strand at 3054.607 kb on - strand at 3054.649 kb on + strand at 3054.649 kb on + strand at 3054.699 kb on - strand at 3054.748 kb on - strand at 3054.850 kb on + strand, within yedD at 3055.061 kb on + strand, within yedD at 3055.076 kb on - strand, within yedD at 3055.108 kb on + strand, within yedD at 3055.116 kb on + strand, within yedD at 3055.312 kb on + strand at 3055.315 kb on + strand at 3055.356 kb on + strand at 3055.363 kb on + strand at 3055.380 kb on + strand at 3055.487 kb on - strand, within amyA at 3055.487 kb on - strand, within amyA at 3055.495 kb on + strand, within amyA at 3055.528 kb on + strand, within amyA at 3055.558 kb on - strand, within amyA at 3055.558 kb on - strand, within amyA at 3055.667 kb on + strand, within amyA at 3055.775 kb on - strand, within amyA at 3055.805 kb on + strand, within amyA at 3055.891 kb on - strand, within amyA at 3055.892 kb on + strand, within amyA at 3055.892 kb on + strand, within amyA at 3055.893 kb on - strand, within amyA at 3055.893 kb on - strand, within amyA at 3055.893 kb on - strand, within amyA at 3055.893 kb on - strand, within amyA at 3055.967 kb on - strand, within amyA at 3056.137 kb on - strand, within amyA at 3056.242 kb on - strand, within amyA at 3056.274 kb on - strand, within amyA at 3056.363 kb on - strand, within amyA at 3056.492 kb on + strand, within amyA at 3056.493 kb on - strand, within amyA at 3056.539 kb on + strand, within amyA at 3056.540 kb on - strand, within amyA at 3056.547 kb on - strand, within amyA at 3056.586 kb on + strand at 3056.586 kb on + strand at 3056.589 kb on - strand at 3056.622 kb on + strand at 3056.628 kb on + strand at 3056.702 kb on - strand at 3056.748 kb on + strand at 3056.748 kb on + strand at 3056.786 kb on - strand at 3056.856 kb on + strand, within fliT at 3056.856 kb on + strand, within fliT at 3056.857 kb on - strand, within fliT at 3056.919 kb on + strand, within fliT at 3056.919 kb on + strand, within fliT at 3056.931 kb on + strand, within fliT at 3056.932 kb on - strand, within fliT at 3057.046 kb on + strand, within fliT at 3057.198 kb on - strand at 3057.301 kb on + strand, within fliS at 3057.302 kb on - strand, within fliS at 3057.303 kb on + strand, within fliS at 3057.303 kb on + strand, within fliS at 3057.303 kb on + strand, within fliS at 3057.304 kb on - strand, within fliS at 3057.304 kb on - strand, within fliS at 3057.304 kb on - strand, within fliS at 3057.304 kb on - strand, within fliS at 3057.307 kb on + strand, within fliS at 3057.308 kb on - strand, within fliS at 3057.310 kb on - strand, within fliS at 3057.440 kb on - strand, within fliS at 3057.440 kb on - strand, within fliS at 3057.482 kb on + strand, within fliS at 3057.570 kb on + strand at 3057.593 kb on + strand at 3057.594 kb on - strand at 3057.600 kb on + strand at 3057.601 kb on - strand at 3057.610 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MPec2 remove 3,054,264 + yedE BNILDI_15155 0.72 -1.0 3,054,293 - yedE BNILDI_15155 0.74 +0.1 3,054,293 - yedE BNILDI_15155 0.74 +0.3 3,054,343 - yedE BNILDI_15155 0.79 +0.1 3,054,374 - yedE BNILDI_15155 0.81 +1.4 3,054,397 - yedE BNILDI_15155 0.83 -0.3 3,054,397 - yedE BNILDI_15155 0.83 -0.3 3,054,509 + +1.7 3,054,516 + -0.9 3,054,565 - +0.3 3,054,606 + +0.3 3,054,607 - +0.1 3,054,649 + +0.9 3,054,649 + +1.4 3,054,699 - +0.8 3,054,748 - +1.0 3,054,850 + yedD BNILDI_15160 0.15 -0.6 3,055,061 + yedD BNILDI_15160 0.66 -1.3 3,055,076 - yedD BNILDI_15160 0.70 +0.7 3,055,108 + yedD BNILDI_15160 0.78 +2.1 3,055,116 + yedD BNILDI_15160 0.79 +1.1 3,055,312 + +1.7 3,055,315 + -1.5 3,055,356 + +1.4 3,055,363 + +0.0 3,055,380 + -0.6 3,055,487 - amyA BNILDI_15165 0.17 +0.5 3,055,487 - amyA BNILDI_15165 0.17 -1.3 3,055,495 + amyA BNILDI_15165 0.18 -1.6 3,055,528 + amyA BNILDI_15165 0.20 +0.1 3,055,558 - amyA BNILDI_15165 0.22 -1.4 3,055,558 - amyA BNILDI_15165 0.22 +1.4 3,055,667 + amyA BNILDI_15165 0.29 -1.8 3,055,775 - amyA BNILDI_15165 0.36 +0.1 3,055,805 + amyA BNILDI_15165 0.38 -1.2 3,055,891 - amyA BNILDI_15165 0.44 -0.3 3,055,892 + amyA BNILDI_15165 0.44 +0.3 3,055,892 + amyA BNILDI_15165 0.44 +2.2 3,055,893 - amyA BNILDI_15165 0.44 +0.1 3,055,893 - amyA BNILDI_15165 0.44 -0.2 3,055,893 - amyA BNILDI_15165 0.44 +0.1 3,055,893 - amyA BNILDI_15165 0.44 +0.4 3,055,967 - amyA BNILDI_15165 0.49 +0.7 3,056,137 - amyA BNILDI_15165 0.61 -0.5 3,056,242 - amyA BNILDI_15165 0.68 +0.2 3,056,274 - amyA BNILDI_15165 0.70 +0.1 3,056,363 - amyA BNILDI_15165 0.76 +0.4 3,056,492 + amyA BNILDI_15165 0.85 -0.0 3,056,493 - amyA BNILDI_15165 0.85 -0.3 3,056,539 + amyA BNILDI_15165 0.88 -1.0 3,056,540 - amyA BNILDI_15165 0.88 +1.7 3,056,547 - amyA BNILDI_15165 0.88 -1.9 3,056,586 + -0.7 3,056,586 + +0.0 3,056,589 - -0.7 3,056,622 + +2.7 3,056,628 + +0.8 3,056,702 - -0.1 3,056,748 + +0.7 3,056,748 + -0.3 3,056,786 - +0.5 3,056,856 + fliT BNILDI_15170 0.16 +1.0 3,056,856 + fliT BNILDI_15170 0.16 -0.8 3,056,857 - fliT BNILDI_15170 0.16 -0.3 3,056,919 + fliT BNILDI_15170 0.33 +0.9 3,056,919 + fliT BNILDI_15170 0.33 -0.3 3,056,931 + fliT BNILDI_15170 0.36 -0.7 3,056,932 - fliT BNILDI_15170 0.36 +1.7 3,057,046 + fliT BNILDI_15170 0.67 +0.1 3,057,198 - +0.4 3,057,301 + fliS BNILDI_15175 0.33 -1.6 3,057,302 - fliS BNILDI_15175 0.34 -1.2 3,057,303 + fliS BNILDI_15175 0.34 +0.1 3,057,303 + fliS BNILDI_15175 0.34 -0.2 3,057,303 + fliS BNILDI_15175 0.34 -0.2 3,057,304 - fliS BNILDI_15175 0.34 -0.0 3,057,304 - fliS BNILDI_15175 0.34 -0.4 3,057,304 - fliS BNILDI_15175 0.34 -0.3 3,057,304 - fliS BNILDI_15175 0.34 +1.3 3,057,307 + fliS BNILDI_15175 0.35 +1.4 3,057,308 - fliS BNILDI_15175 0.35 -0.3 3,057,310 - fliS BNILDI_15175 0.36 -1.1 3,057,440 - fliS BNILDI_15175 0.67 +2.4 3,057,440 - fliS BNILDI_15175 0.67 -0.9 3,057,482 + fliS BNILDI_15175 0.77 +0.3 3,057,570 + -0.0 3,057,593 + +1.0 3,057,594 - -0.1 3,057,600 + -1.0 3,057,601 - -0.3 3,057,610 + -1.8
Or see this region's nucleotide sequence