Strain Fitness in Escherichia coli ECRC62 around BNILDI_14735

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisB and hisC overlap by 1 nucleotideshisC and hisD overlap by 4 nucleotides BNILDI_14730: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 2,975,535 to 2,976,602 hisB BNILDI_14735: hisC - histidinol-phosphate transaminase, at 2,976,602 to 2,977,672 hisC BNILDI_14740: hisD - Histidinol dehydrogenase, at 2,977,669 to 2,978,973 hisD Position (kb) 2976 2977 2978Strain fitness (log2 ratio) -1 0 1 2at 2975.625 kb on + strandat 2975.683 kb on + strand, within hisBat 2975.748 kb on - strand, within hisBat 2975.760 kb on - strand, within hisBat 2975.760 kb on - strand, within hisBat 2975.760 kb on - strand, within hisBat 2976.091 kb on - strand, within hisBat 2976.091 kb on - strand, within hisBat 2976.231 kb on - strand, within hisBat 2976.231 kb on - strand, within hisBat 2976.590 kb on - strandat 2976.697 kb on + strandat 2976.716 kb on - strand, within hisCat 2976.760 kb on + strand, within hisCat 2976.857 kb on - strand, within hisCat 2977.109 kb on + strand, within hisCat 2977.112 kb on - strand, within hisCat 2977.113 kb on + strand, within hisCat 2977.113 kb on + strand, within hisCat 2977.113 kb on + strand, within hisCat 2977.114 kb on - strand, within hisCat 2977.152 kb on - strand, within hisCat 2977.221 kb on + strand, within hisCat 2977.221 kb on + strand, within hisCat 2977.336 kb on - strand, within hisCat 2977.465 kb on - strand, within hisCat 2977.531 kb on + strand, within hisCat 2977.531 kb on + strand, within hisCat 2977.646 kb on + strandat 2977.657 kb on + strandat 2977.657 kb on + strandat 2977.684 kb on - strandat 2977.781 kb on - strandat 2977.860 kb on - strand, within hisDat 2978.024 kb on - strand, within hisDat 2978.549 kb on - strand, within hisDat 2978.584 kb on - strand, within hisDat 2978.585 kb on + strand, within hisDat 2978.586 kb on - strand, within hisDat 2978.586 kb on - strand, within hisDat 2978.586 kb on - strand, within hisDat 2978.586 kb on - strand, within hisDat 2978.647 kb on + strand, within hisD

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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2,975,625 + -0.6
2,975,683 + hisB BNILDI_14730 0.14 +0.3
2,975,748 - hisB BNILDI_14730 0.20 -0.3
2,975,760 - hisB BNILDI_14730 0.21 -1.3
2,975,760 - hisB BNILDI_14730 0.21 +0.1
2,975,760 - hisB BNILDI_14730 0.21 +1.7
2,976,091 - hisB BNILDI_14730 0.52 -0.9
2,976,091 - hisB BNILDI_14730 0.52 -0.8
2,976,231 - hisB BNILDI_14730 0.65 +1.1
2,976,231 - hisB BNILDI_14730 0.65 +0.1
2,976,590 - -0.0
2,976,697 + +0.2
2,976,716 - hisC BNILDI_14735 0.11 -0.6
2,976,760 + hisC BNILDI_14735 0.15 +0.1
2,976,857 - hisC BNILDI_14735 0.24 -0.7
2,977,109 + hisC BNILDI_14735 0.47 -1.0
2,977,112 - hisC BNILDI_14735 0.48 +0.2
2,977,113 + hisC BNILDI_14735 0.48 +0.7
2,977,113 + hisC BNILDI_14735 0.48 -0.6
2,977,113 + hisC BNILDI_14735 0.48 -0.4
2,977,114 - hisC BNILDI_14735 0.48 +2.7
2,977,152 - hisC BNILDI_14735 0.51 +0.1
2,977,221 + hisC BNILDI_14735 0.58 +1.1
2,977,221 + hisC BNILDI_14735 0.58 +0.4
2,977,336 - hisC BNILDI_14735 0.69 +1.3
2,977,465 - hisC BNILDI_14735 0.81 -0.1
2,977,531 + hisC BNILDI_14735 0.87 -0.0
2,977,531 + hisC BNILDI_14735 0.87 +0.1
2,977,646 + -0.1
2,977,657 + -1.3
2,977,657 + -0.5
2,977,684 - +1.1
2,977,781 - +0.7
2,977,860 - hisD BNILDI_14740 0.15 -1.1
2,978,024 - hisD BNILDI_14740 0.27 -0.4
2,978,549 - hisD BNILDI_14740 0.67 +1.3
2,978,584 - hisD BNILDI_14740 0.70 -0.4
2,978,585 + hisD BNILDI_14740 0.70 -0.4
2,978,586 - hisD BNILDI_14740 0.70 -0.8
2,978,586 - hisD BNILDI_14740 0.70 -0.4
2,978,586 - hisD BNILDI_14740 0.70 -0.5
2,978,586 - hisD BNILDI_14740 0.70 +0.9
2,978,647 + hisD BNILDI_14740 0.75 -0.5

Or see this region's nucleotide sequence