Strain Fitness in Escherichia coli ECRC62 around BNILDI_14490

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntibsA and terY are separated by 220 nucleotidesterY and pphC are separated by 77 nucleotidespphC and yegI overlap by 4 nucleotides BNILDI_14480: ibsA - Small toxic protein IbsA, at 2,921,874 to 2,921,933 ibsA BNILDI_14485: terY - Uncharacterized protein YegL, at 2,922,154 to 2,922,813 terY BNILDI_14490: pphC - Serine/threonine-protein phosphatase 3, at 2,922,891 to 2,923,571 pphC BNILDI_14495: yegI - protein kinase YegI, at 2,923,568 to 2,925,508 yegI Position (kb) 2922 2923 2924Strain fitness (log2 ratio) -2 -1 0 1 2at 2921.915 kb on + strand, within ibsAat 2921.929 kb on - strandat 2921.931 kb on + strandat 2921.931 kb on + strandat 2921.932 kb on - strandat 2921.932 kb on - strandat 2921.999 kb on + strandat 2921.999 kb on + strandat 2922.038 kb on - strandat 2922.065 kb on - strandat 2922.092 kb on + strandat 2922.262 kb on - strand, within terYat 2922.265 kb on - strand, within terYat 2922.274 kb on - strand, within terYat 2922.514 kb on + strand, within terYat 2922.607 kb on - strand, within terYat 2922.829 kb on - strandat 2922.868 kb on + strandat 2922.868 kb on + strandat 2922.869 kb on - strandat 2923.011 kb on + strand, within pphCat 2923.012 kb on - strand, within pphCat 2923.012 kb on - strand, within pphCat 2923.012 kb on - strand, within pphCat 2923.013 kb on + strand, within pphCat 2923.014 kb on - strand, within pphCat 2923.049 kb on + strand, within pphCat 2923.267 kb on - strand, within pphCat 2923.267 kb on - strand, within pphCat 2923.267 kb on - strand, within pphCat 2923.274 kb on + strand, within pphCat 2923.275 kb on - strand, within pphCat 2923.391 kb on + strand, within pphCat 2923.391 kb on + strand, within pphCat 2923.392 kb on - strand, within pphCat 2923.392 kb on - strand, within pphCat 2923.400 kb on + strand, within pphCat 2923.415 kb on - strand, within pphCat 2923.475 kb on - strand, within pphCat 2923.539 kb on - strandat 2923.581 kb on + strandat 2923.581 kb on + strandat 2923.582 kb on - strandat 2923.582 kb on - strandat 2923.585 kb on + strandat 2923.585 kb on + strandat 2923.585 kb on + strandat 2923.650 kb on - strandat 2923.650 kb on - strandat 2923.689 kb on - strandat 2923.766 kb on + strand, within yegIat 2923.767 kb on - strand, within yegIat 2923.767 kb on - strand, within yegIat 2923.767 kb on - strand, within yegIat 2923.847 kb on - strand, within yegIat 2923.862 kb on - strand, within yegIat 2923.862 kb on - strand, within yegIat 2923.868 kb on + strand, within yegIat 2923.868 kb on + strand, within yegIat 2923.869 kb on - strand, within yegIat 2923.869 kb on - strand, within yegIat 2923.920 kb on + strand, within yegIat 2923.920 kb on + strand, within yegIat 2923.926 kb on - strand, within yegIat 2924.245 kb on + strand, within yegIat 2924.437 kb on + strand, within yegIat 2924.542 kb on + strand, within yegI

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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2,921,915 + ibsA BNILDI_14480 0.68 -0.1
2,921,929 - -0.7
2,921,931 + +0.3
2,921,931 + +0.7
2,921,932 - +0.2
2,921,932 - +0.5
2,921,999 + +0.8
2,921,999 + +0.7
2,922,038 - +0.9
2,922,065 - -0.8
2,922,092 + +1.5
2,922,262 - terY BNILDI_14485 0.16 +0.8
2,922,265 - terY BNILDI_14485 0.17 +0.2
2,922,274 - terY BNILDI_14485 0.18 +0.4
2,922,514 + terY BNILDI_14485 0.55 -0.7
2,922,607 - terY BNILDI_14485 0.69 +0.4
2,922,829 - +0.7
2,922,868 + +0.6
2,922,868 + +0.7
2,922,869 - -0.3
2,923,011 + pphC BNILDI_14490 0.18 +0.1
2,923,012 - pphC BNILDI_14490 0.18 +1.7
2,923,012 - pphC BNILDI_14490 0.18 +0.1
2,923,012 - pphC BNILDI_14490 0.18 -0.6
2,923,013 + pphC BNILDI_14490 0.18 -1.3
2,923,014 - pphC BNILDI_14490 0.18 +0.1
2,923,049 + pphC BNILDI_14490 0.23 +1.0
2,923,267 - pphC BNILDI_14490 0.55 -0.3
2,923,267 - pphC BNILDI_14490 0.55 -1.6
2,923,267 - pphC BNILDI_14490 0.55 +1.4
2,923,274 + pphC BNILDI_14490 0.56 -1.7
2,923,275 - pphC BNILDI_14490 0.56 +1.0
2,923,391 + pphC BNILDI_14490 0.73 -0.9
2,923,391 + pphC BNILDI_14490 0.73 -0.3
2,923,392 - pphC BNILDI_14490 0.74 -0.8
2,923,392 - pphC BNILDI_14490 0.74 +2.0
2,923,400 + pphC BNILDI_14490 0.75 -0.6
2,923,415 - pphC BNILDI_14490 0.77 -0.7
2,923,475 - pphC BNILDI_14490 0.86 -0.4
2,923,539 - +0.0
2,923,581 + -0.2
2,923,581 + +0.3
2,923,582 - +0.1
2,923,582 - -2.1
2,923,585 + -0.9
2,923,585 + +0.8
2,923,585 + -0.5
2,923,650 - +0.4
2,923,650 - +0.9
2,923,689 - +0.8
2,923,766 + yegI BNILDI_14495 0.10 -1.8
2,923,767 - yegI BNILDI_14495 0.10 +1.7
2,923,767 - yegI BNILDI_14495 0.10 +0.7
2,923,767 - yegI BNILDI_14495 0.10 +0.1
2,923,847 - yegI BNILDI_14495 0.14 -0.5
2,923,862 - yegI BNILDI_14495 0.15 +0.7
2,923,862 - yegI BNILDI_14495 0.15 -0.3
2,923,868 + yegI BNILDI_14495 0.15 -0.9
2,923,868 + yegI BNILDI_14495 0.15 +1.7
2,923,869 - yegI BNILDI_14495 0.16 -1.4
2,923,869 - yegI BNILDI_14495 0.16 +0.4
2,923,920 + yegI BNILDI_14495 0.18 -0.6
2,923,920 + yegI BNILDI_14495 0.18 +0.4
2,923,926 - yegI BNILDI_14495 0.18 -0.3
2,924,245 + yegI BNILDI_14495 0.35 -0.4
2,924,437 + yegI BNILDI_14495 0.45 +0.0
2,924,542 + yegI BNILDI_14495 0.50 +0.3

Or see this region's nucleotide sequence