Strain Fitness in Escherichia coli ECRC62 around BNILDI_12260

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntppx and ppk1 are separated by 4 nucleotidesppk1 and purN are separated by 170 nucleotidespurN and purM overlap by 1 nucleotides BNILDI_12255: ppx - exopolyphosphatase, at 2,459,690 to 2,461,231 ppx BNILDI_12260: ppk1 - polyphosphate kinase 1, at 2,461,236 to 2,463,302 ppk1 BNILDI_12265: purN - phosphoribosylglycinamide formyltransferase, at 2,463,473 to 2,464,111 purN BNILDI_12270: purM - Phosphoribosylformylglycinamidine cyclo-ligase, at 2,464,111 to 2,465,148 purM Position (kb) 2461 2462 2463 2464Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2460.325 kb on - strand, within ppxat 2460.342 kb on - strand, within ppxat 2460.377 kb on + strand, within ppxat 2460.706 kb on - strand, within ppxat 2460.706 kb on - strand, within ppxat 2460.755 kb on - strand, within ppxat 2460.838 kb on + strand, within ppxat 2460.838 kb on + strand, within ppxat 2460.974 kb on + strand, within ppxat 2460.975 kb on - strand, within ppxat 2461.066 kb on + strand, within ppxat 2461.066 kb on + strand, within ppxat 2461.066 kb on + strand, within ppxat 2461.069 kb on + strand, within ppxat 2461.069 kb on + strand, within ppxat 2461.140 kb on - strandat 2461.152 kb on - strandat 2461.152 kb on - strandat 2461.216 kb on + strandat 2461.265 kb on - strandat 2461.270 kb on + strandat 2461.270 kb on + strandat 2461.271 kb on - strandat 2461.271 kb on - strandat 2461.374 kb on + strandat 2461.375 kb on - strandat 2461.375 kb on - strandat 2461.375 kb on - strandat 2461.538 kb on - strand, within ppk1at 2461.550 kb on + strand, within ppk1at 2461.655 kb on - strand, within ppk1at 2461.688 kb on + strand, within ppk1at 2461.689 kb on - strand, within ppk1at 2461.693 kb on - strand, within ppk1at 2461.693 kb on - strand, within ppk1at 2461.749 kb on + strand, within ppk1at 2461.750 kb on - strand, within ppk1at 2461.760 kb on - strand, within ppk1at 2461.794 kb on + strand, within ppk1at 2461.795 kb on - strand, within ppk1at 2461.838 kb on + strand, within ppk1at 2462.027 kb on + strand, within ppk1at 2462.028 kb on - strand, within ppk1at 2462.073 kb on - strand, within ppk1at 2462.124 kb on - strand, within ppk1at 2462.130 kb on - strand, within ppk1at 2462.264 kb on - strand, within ppk1at 2462.272 kb on + strand, within ppk1at 2462.340 kb on - strand, within ppk1at 2462.340 kb on - strand, within ppk1at 2462.403 kb on - strand, within ppk1at 2462.459 kb on + strand, within ppk1at 2462.538 kb on + strand, within ppk1at 2462.599 kb on - strand, within ppk1at 2462.635 kb on + strand, within ppk1at 2462.635 kb on + strand, within ppk1at 2462.842 kb on + strand, within ppk1at 2462.843 kb on - strand, within ppk1at 2462.843 kb on - strand, within ppk1at 2462.914 kb on + strand, within ppk1at 2463.010 kb on - strand, within ppk1at 2463.019 kb on + strand, within ppk1at 2463.019 kb on + strand, within ppk1at 2463.154 kb on + strandat 2463.155 kb on - strandat 2463.178 kb on + strandat 2463.283 kb on + strandat 2463.283 kb on + strandat 2463.284 kb on - strandat 2463.286 kb on - strandat 2463.313 kb on - strandat 2463.344 kb on + strandat 2463.344 kb on + strandat 2463.347 kb on + strandat 2463.377 kb on + strandat 2463.464 kb on + strandat 2463.568 kb on + strand, within purNat 2463.712 kb on + strand, within purNat 2463.768 kb on + strand, within purNat 2463.879 kb on + strand, within purNat 2463.880 kb on - strand, within purNat 2463.880 kb on - strand, within purNat 2463.880 kb on - strand, within purNat 2463.880 kb on - strand, within purNat 2463.880 kb on - strand, within purNat 2463.913 kb on - strand, within purNat 2464.042 kb on + strand, within purNat 2464.061 kb on - strandat 2464.093 kb on + strandat 2464.093 kb on + strandat 2464.094 kb on - strandat 2464.106 kb on - strandat 2464.266 kb on - strand, within purMat 2464.288 kb on - strand, within purM

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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2,460,325 - ppx BNILDI_12255 0.41 +0.5
2,460,342 - ppx BNILDI_12255 0.42 +0.2
2,460,377 + ppx BNILDI_12255 0.45 +0.1
2,460,706 - ppx BNILDI_12255 0.66 -0.4
2,460,706 - ppx BNILDI_12255 0.66 +0.2
2,460,755 - ppx BNILDI_12255 0.69 +1.9
2,460,838 + ppx BNILDI_12255 0.74 -0.3
2,460,838 + ppx BNILDI_12255 0.74 +1.3
2,460,974 + ppx BNILDI_12255 0.83 -2.1
2,460,975 - ppx BNILDI_12255 0.83 -0.5
2,461,066 + ppx BNILDI_12255 0.89 +0.6
2,461,066 + ppx BNILDI_12255 0.89 +0.3
2,461,066 + ppx BNILDI_12255 0.89 -0.8
2,461,069 + ppx BNILDI_12255 0.89 -2.5
2,461,069 + ppx BNILDI_12255 0.89 +0.4
2,461,140 - -0.2
2,461,152 - +1.3
2,461,152 - -0.2
2,461,216 + -0.7
2,461,265 - +0.2
2,461,270 + +0.5
2,461,270 + -0.5
2,461,271 - -0.1
2,461,271 - +0.0
2,461,374 + +1.9
2,461,375 - +0.2
2,461,375 - -0.7
2,461,375 - +0.0
2,461,538 - ppk1 BNILDI_12260 0.15 -0.6
2,461,550 + ppk1 BNILDI_12260 0.15 +0.2
2,461,655 - ppk1 BNILDI_12260 0.20 +0.3
2,461,688 + ppk1 BNILDI_12260 0.22 -0.2
2,461,689 - ppk1 BNILDI_12260 0.22 -0.0
2,461,693 - ppk1 BNILDI_12260 0.22 +0.5
2,461,693 - ppk1 BNILDI_12260 0.22 +0.4
2,461,749 + ppk1 BNILDI_12260 0.25 +0.2
2,461,750 - ppk1 BNILDI_12260 0.25 +0.3
2,461,760 - ppk1 BNILDI_12260 0.25 -1.5
2,461,794 + ppk1 BNILDI_12260 0.27 +1.3
2,461,795 - ppk1 BNILDI_12260 0.27 +1.5
2,461,838 + ppk1 BNILDI_12260 0.29 +0.1
2,462,027 + ppk1 BNILDI_12260 0.38 -0.3
2,462,028 - ppk1 BNILDI_12260 0.38 -0.1
2,462,073 - ppk1 BNILDI_12260 0.40 +1.3
2,462,124 - ppk1 BNILDI_12260 0.43 -0.1
2,462,130 - ppk1 BNILDI_12260 0.43 +0.3
2,462,264 - ppk1 BNILDI_12260 0.50 +0.1
2,462,272 + ppk1 BNILDI_12260 0.50 -0.1
2,462,340 - ppk1 BNILDI_12260 0.53 -0.8
2,462,340 - ppk1 BNILDI_12260 0.53 -0.5
2,462,403 - ppk1 BNILDI_12260 0.56 -0.2
2,462,459 + ppk1 BNILDI_12260 0.59 -1.1
2,462,538 + ppk1 BNILDI_12260 0.63 +0.0
2,462,599 - ppk1 BNILDI_12260 0.66 +0.1
2,462,635 + ppk1 BNILDI_12260 0.68 -0.5
2,462,635 + ppk1 BNILDI_12260 0.68 +0.1
2,462,842 + ppk1 BNILDI_12260 0.78 +2.3
2,462,843 - ppk1 BNILDI_12260 0.78 -0.3
2,462,843 - ppk1 BNILDI_12260 0.78 +0.5
2,462,914 + ppk1 BNILDI_12260 0.81 -1.3
2,463,010 - ppk1 BNILDI_12260 0.86 +0.3
2,463,019 + ppk1 BNILDI_12260 0.86 -1.3
2,463,019 + ppk1 BNILDI_12260 0.86 +0.9
2,463,154 + +0.1
2,463,155 - +0.0
2,463,178 + -0.1
2,463,283 + +1.9
2,463,283 + -0.3
2,463,284 - +1.9
2,463,286 - -0.9
2,463,313 - -0.8
2,463,344 + -0.2
2,463,344 + -0.2
2,463,347 + +1.0
2,463,377 + -0.7
2,463,464 + -0.1
2,463,568 + purN BNILDI_12265 0.15 -0.9
2,463,712 + purN BNILDI_12265 0.37 -0.4
2,463,768 + purN BNILDI_12265 0.46 +1.9
2,463,879 + purN BNILDI_12265 0.64 +0.5
2,463,880 - purN BNILDI_12265 0.64 -0.5
2,463,880 - purN BNILDI_12265 0.64 +0.9
2,463,880 - purN BNILDI_12265 0.64 +3.7
2,463,880 - purN BNILDI_12265 0.64 +0.4
2,463,880 - purN BNILDI_12265 0.64 -0.1
2,463,913 - purN BNILDI_12265 0.69 -0.1
2,464,042 + purN BNILDI_12265 0.89 -0.4
2,464,061 - +0.1
2,464,093 + +1.2
2,464,093 + +0.3
2,464,094 - -1.3
2,464,106 - -0.3
2,464,266 - purM BNILDI_12270 0.15 -0.7
2,464,288 - purM BNILDI_12270 0.17 -0.8

Or see this region's nucleotide sequence