Strain Fitness in Escherichia coli ECRC62 around BNILDI_12085

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrmJ and iscR are separated by 300 nucleotidesiscR and iscS are separated by 111 nucleotides BNILDI_12080: trmJ - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 2,422,240 to 2,422,977 trmJ BNILDI_12085: iscR - Fe-S cluster assembly transcriptional regulator IscR, at 2,423,278 to 2,423,766 iscR BNILDI_12090: iscS - cysteine desulfurase, at 2,423,878 to 2,425,092 iscS Position (kb) 2423 2424Strain fitness (log2 ratio) -1 0 1 2 3at 2422.290 kb on + strandat 2422.413 kb on + strand, within trmJat 2422.413 kb on + strand, within trmJat 2422.414 kb on - strand, within trmJat 2422.432 kb on - strand, within trmJat 2422.458 kb on + strand, within trmJat 2422.459 kb on - strand, within trmJat 2422.657 kb on + strand, within trmJat 2422.657 kb on + strand, within trmJat 2422.769 kb on - strand, within trmJat 2422.799 kb on - strand, within trmJat 2422.945 kb on - strandat 2422.966 kb on - strandat 2422.966 kb on - strandat 2422.991 kb on + strandat 2423.066 kb on + strandat 2423.147 kb on - strandat 2423.219 kb on + strandat 2423.219 kb on + strandat 2423.231 kb on + strandat 2423.231 kb on + strandat 2423.272 kb on + strandat 2423.272 kb on + strandat 2423.292 kb on + strandat 2423.292 kb on + strandat 2423.292 kb on + strandat 2423.367 kb on + strand, within iscRat 2423.427 kb on + strand, within iscRat 2423.450 kb on + strand, within iscRat 2423.513 kb on + strand, within iscRat 2423.649 kb on + strand, within iscRat 2423.649 kb on + strand, within iscRat 2423.649 kb on + strand, within iscRat 2423.751 kb on + strandat 2423.808 kb on + strandat 2423.820 kb on + strandat 2423.860 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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2,422,290 + -0.1
2,422,413 + trmJ BNILDI_12080 0.23 -0.2
2,422,413 + trmJ BNILDI_12080 0.23 -0.3
2,422,414 - trmJ BNILDI_12080 0.24 +0.4
2,422,432 - trmJ BNILDI_12080 0.26 +0.9
2,422,458 + trmJ BNILDI_12080 0.30 -0.3
2,422,459 - trmJ BNILDI_12080 0.30 -0.5
2,422,657 + trmJ BNILDI_12080 0.57 +1.1
2,422,657 + trmJ BNILDI_12080 0.57 +0.9
2,422,769 - trmJ BNILDI_12080 0.72 +0.9
2,422,799 - trmJ BNILDI_12080 0.76 -0.9
2,422,945 - +0.7
2,422,966 - -0.6
2,422,966 - -1.1
2,422,991 + -0.4
2,423,066 + +0.1
2,423,147 - -0.2
2,423,219 + +1.8
2,423,219 + -0.0
2,423,231 + -0.0
2,423,231 + -0.0
2,423,272 + +1.9
2,423,272 + -0.3
2,423,292 + +1.1
2,423,292 + +2.5
2,423,292 + -0.9
2,423,367 + iscR BNILDI_12085 0.18 -0.4
2,423,427 + iscR BNILDI_12085 0.30 -0.4
2,423,450 + iscR BNILDI_12085 0.35 +0.2
2,423,513 + iscR BNILDI_12085 0.48 +0.0
2,423,649 + iscR BNILDI_12085 0.76 +0.1
2,423,649 + iscR BNILDI_12085 0.76 +0.2
2,423,649 + iscR BNILDI_12085 0.76 -0.9
2,423,751 + -0.4
2,423,808 + -0.6
2,423,820 + +2.9
2,423,860 + +0.6

Or see this region's nucleotide sequence