Experiment: MPec2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmJ and iscR are separated by 300 nucleotides iscR and iscS are separated by 111 nucleotides
BNILDI_12080: trmJ - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 2,422,240 to 2,422,977
trmJ
BNILDI_12085: iscR - Fe-S cluster assembly transcriptional regulator IscR, at 2,423,278 to 2,423,766
iscR
BNILDI_12090: iscS - cysteine desulfurase, at 2,423,878 to 2,425,092
iscS
Position (kb)
2423
2424 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2422.290 kb on + strand at 2422.413 kb on + strand, within trmJ at 2422.413 kb on + strand, within trmJ at 2422.414 kb on - strand, within trmJ at 2422.432 kb on - strand, within trmJ at 2422.458 kb on + strand, within trmJ at 2422.459 kb on - strand, within trmJ at 2422.657 kb on + strand, within trmJ at 2422.657 kb on + strand, within trmJ at 2422.769 kb on - strand, within trmJ at 2422.799 kb on - strand, within trmJ at 2422.945 kb on - strand at 2422.966 kb on - strand at 2422.966 kb on - strand at 2422.991 kb on + strand at 2423.066 kb on + strand at 2423.147 kb on - strand at 2423.219 kb on + strand at 2423.219 kb on + strand at 2423.231 kb on + strand at 2423.231 kb on + strand at 2423.272 kb on + strand at 2423.272 kb on + strand at 2423.292 kb on + strand at 2423.292 kb on + strand at 2423.292 kb on + strand at 2423.367 kb on + strand, within iscR at 2423.427 kb on + strand, within iscR at 2423.450 kb on + strand, within iscR at 2423.513 kb on + strand, within iscR at 2423.649 kb on + strand, within iscR at 2423.649 kb on + strand, within iscR at 2423.649 kb on + strand, within iscR at 2423.751 kb on + strand at 2423.808 kb on + strand at 2423.820 kb on + strand at 2423.860 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MPec2 remove 2,422,290 + -0.1 2,422,413 + trmJ BNILDI_12080 0.23 -0.2 2,422,413 + trmJ BNILDI_12080 0.23 -0.3 2,422,414 - trmJ BNILDI_12080 0.24 +0.4 2,422,432 - trmJ BNILDI_12080 0.26 +0.9 2,422,458 + trmJ BNILDI_12080 0.30 -0.3 2,422,459 - trmJ BNILDI_12080 0.30 -0.5 2,422,657 + trmJ BNILDI_12080 0.57 +1.1 2,422,657 + trmJ BNILDI_12080 0.57 +0.9 2,422,769 - trmJ BNILDI_12080 0.72 +0.9 2,422,799 - trmJ BNILDI_12080 0.76 -0.9 2,422,945 - +0.7 2,422,966 - -0.6 2,422,966 - -1.1 2,422,991 + -0.4 2,423,066 + +0.1 2,423,147 - -0.2 2,423,219 + +1.8 2,423,219 + -0.0 2,423,231 + -0.0 2,423,231 + -0.0 2,423,272 + +1.9 2,423,272 + -0.3 2,423,292 + +1.1 2,423,292 + +2.5 2,423,292 + -0.9 2,423,367 + iscR BNILDI_12085 0.18 -0.4 2,423,427 + iscR BNILDI_12085 0.30 -0.4 2,423,450 + iscR BNILDI_12085 0.35 +0.2 2,423,513 + iscR BNILDI_12085 0.48 +0.0 2,423,649 + iscR BNILDI_12085 0.76 +0.1 2,423,649 + iscR BNILDI_12085 0.76 +0.2 2,423,649 + iscR BNILDI_12085 0.76 -0.9 2,423,751 + -0.4 2,423,808 + -0.6 2,423,820 + +2.9 2,423,860 + +0.6
Or see this region's nucleotide sequence