Strain Fitness in Escherichia coli ECRC62 around BNILDI_11215

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntproX and proW are separated by 57 nucleotidesproW and proV overlap by 8 nucleotides BNILDI_11210: proX - glycine betaine/L-proline ABC transporter substrate-binding protein ProX, at 2,259,583 to 2,260,575 proX BNILDI_11215: proW - glycine betaine/L-proline ABC transporter permease ProW, at 2,260,633 to 2,261,697 proW BNILDI_11220: proV - glycine betaine/L-proline ABC transporter ATP-binding protein ProV, at 2,261,690 to 2,262,892 proV Position (kb) 2260 2261 2262Strain fitness (log2 ratio) -2 -1 0 1 2at 2259.699 kb on + strand, within proXat 2259.699 kb on + strand, within proXat 2259.720 kb on + strand, within proXat 2259.720 kb on + strand, within proXat 2259.720 kb on + strand, within proXat 2259.720 kb on + strand, within proXat 2259.721 kb on - strand, within proXat 2259.801 kb on + strand, within proXat 2259.802 kb on - strand, within proXat 2259.959 kb on + strand, within proXat 2259.960 kb on - strand, within proXat 2260.104 kb on + strand, within proXat 2260.105 kb on - strand, within proXat 2260.115 kb on - strand, within proXat 2260.125 kb on + strand, within proXat 2260.194 kb on + strand, within proXat 2260.227 kb on - strand, within proXat 2260.295 kb on + strand, within proXat 2260.296 kb on - strand, within proXat 2260.382 kb on - strand, within proXat 2260.404 kb on + strand, within proXat 2260.407 kb on - strand, within proXat 2260.412 kb on - strand, within proXat 2260.488 kb on + strandat 2260.488 kb on + strandat 2260.492 kb on - strandat 2260.492 kb on - strandat 2260.492 kb on - strandat 2260.492 kb on - strandat 2260.534 kb on - strandat 2260.566 kb on + strandat 2260.566 kb on + strandat 2260.567 kb on - strandat 2260.579 kb on + strandat 2260.635 kb on - strandat 2260.816 kb on + strand, within proWat 2260.817 kb on - strand, within proWat 2260.866 kb on + strand, within proWat 2260.866 kb on + strand, within proWat 2260.928 kb on - strand, within proWat 2261.368 kb on - strand, within proWat 2261.423 kb on + strand, within proWat 2261.423 kb on + strand, within proWat 2261.423 kb on + strand, within proWat 2261.423 kb on + strand, within proWat 2261.424 kb on - strand, within proWat 2261.424 kb on - strand, within proWat 2261.544 kb on + strand, within proWat 2261.622 kb on + strandat 2261.670 kb on + strandat 2261.699 kb on - strandat 2261.717 kb on + strandat 2261.757 kb on + strandat 2261.757 kb on + strandat 2261.758 kb on - strandat 2261.758 kb on - strandat 2261.758 kb on - strandat 2261.758 kb on - strandat 2261.959 kb on - strand, within proVat 2262.025 kb on + strand, within proVat 2262.026 kb on - strand, within proVat 2262.681 kb on - strand, within proV

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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2,259,699 + proX BNILDI_11210 0.12 +0.2
2,259,699 + proX BNILDI_11210 0.12 -0.4
2,259,720 + proX BNILDI_11210 0.14 +1.9
2,259,720 + proX BNILDI_11210 0.14 +0.1
2,259,720 + proX BNILDI_11210 0.14 -0.3
2,259,720 + proX BNILDI_11210 0.14 +0.6
2,259,721 - proX BNILDI_11210 0.14 -1.0
2,259,801 + proX BNILDI_11210 0.22 -0.2
2,259,802 - proX BNILDI_11210 0.22 -0.5
2,259,959 + proX BNILDI_11210 0.38 +1.3
2,259,960 - proX BNILDI_11210 0.38 -0.5
2,260,104 + proX BNILDI_11210 0.52 +0.5
2,260,105 - proX BNILDI_11210 0.53 +2.7
2,260,115 - proX BNILDI_11210 0.54 -1.0
2,260,125 + proX BNILDI_11210 0.55 +1.1
2,260,194 + proX BNILDI_11210 0.62 -0.6
2,260,227 - proX BNILDI_11210 0.65 +0.6
2,260,295 + proX BNILDI_11210 0.72 -0.8
2,260,296 - proX BNILDI_11210 0.72 -0.1
2,260,382 - proX BNILDI_11210 0.80 -1.0
2,260,404 + proX BNILDI_11210 0.83 +0.6
2,260,407 - proX BNILDI_11210 0.83 -0.5
2,260,412 - proX BNILDI_11210 0.83 -0.3
2,260,488 + -0.5
2,260,488 + +1.1
2,260,492 - +0.9
2,260,492 - -0.4
2,260,492 - -0.1
2,260,492 - +0.6
2,260,534 - -1.0
2,260,566 + +0.5
2,260,566 + +1.9
2,260,567 - -0.1
2,260,579 + -0.1
2,260,635 - -0.2
2,260,816 + proW BNILDI_11215 0.17 +0.3
2,260,817 - proW BNILDI_11215 0.17 -1.1
2,260,866 + proW BNILDI_11215 0.22 -0.0
2,260,866 + proW BNILDI_11215 0.22 -0.5
2,260,928 - proW BNILDI_11215 0.28 +0.9
2,261,368 - proW BNILDI_11215 0.69 +1.2
2,261,423 + proW BNILDI_11215 0.74 +0.4
2,261,423 + proW BNILDI_11215 0.74 -1.0
2,261,423 + proW BNILDI_11215 0.74 -0.3
2,261,423 + proW BNILDI_11215 0.74 +0.6
2,261,424 - proW BNILDI_11215 0.74 +0.2
2,261,424 - proW BNILDI_11215 0.74 +0.2
2,261,544 + proW BNILDI_11215 0.86 -1.0
2,261,622 + +1.4
2,261,670 + -0.1
2,261,699 - +0.1
2,261,717 + +0.0
2,261,757 + +0.1
2,261,757 + +1.9
2,261,758 - +2.5
2,261,758 - +0.5
2,261,758 - -2.3
2,261,758 - -0.1
2,261,959 - proV BNILDI_11220 0.22 -0.2
2,262,025 + proV BNILDI_11220 0.28 +0.1
2,262,026 - proV BNILDI_11220 0.28 -0.7
2,262,681 - proV BNILDI_11220 0.82 +1.9

Or see this region's nucleotide sequence