Strain Fitness in Escherichia coli ECRC62 around BNILDI_11150

Experiment: MPec2

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntArg_trna and Arg_trna are separated by 196 nucleotidesArg_trna and Arg_trna are separated by 198 nucleotidesArg_trna and yqaB are separated by 280 nucleotidesyqaB and ygaA overlap by 4 nucleotidesygaA and gshA are separated by 72 nucleotides BNILDI_11135: Arg_trna - tRNA-Arg(acg), at 2,249,292 to 2,249,368 _trna BNILDI_11140: Arg_trna - tRNA-Arg(acg), at 2,249,565 to 2,249,641 _trna BNILDI_11145: Arg_trna - tRNA-Arg(acg), at 2,249,840 to 2,249,916 _trna BNILDI_11150: yqaB - fructose-1-phosphate/6-phosphogluconate phosphatase, at 2,250,197 to 2,250,763 yqaB BNILDI_11155: ygaA - SNARE associated Golgi protein, at 2,250,760 to 2,251,188 ygaA BNILDI_11160: gshA - glutamate--cysteine ligase, at 2,251,261 to 2,252,817 gshA Position (kb) 2250 2251Strain fitness (log2 ratio) -2 -1 0 1 2at 2249.514 kb on + strandat 2249.514 kb on + strandat 2249.690 kb on + strandat 2249.917 kb on + strandat 2249.917 kb on + strandat 2249.962 kb on + strandat 2249.962 kb on + strandat 2249.965 kb on + strandat 2250.156 kb on - strandat 2250.165 kb on + strandat 2250.200 kb on + strandat 2250.201 kb on - strandat 2250.220 kb on - strandat 2250.286 kb on - strand, within yqaBat 2250.355 kb on + strand, within yqaBat 2250.439 kb on + strand, within yqaBat 2250.439 kb on + strand, within yqaBat 2250.659 kb on - strand, within yqaBat 2250.813 kb on + strand, within ygaAat 2250.875 kb on - strand, within ygaAat 2250.912 kb on + strand, within ygaAat 2250.913 kb on - strand, within ygaAat 2250.913 kb on - strand, within ygaAat 2250.931 kb on - strand, within ygaAat 2250.931 kb on - strand, within ygaAat 2250.964 kb on - strand, within ygaAat 2251.026 kb on - strand, within ygaAat 2251.029 kb on - strand, within ygaAat 2251.031 kb on + strand, within ygaAat 2251.058 kb on + strand, within ygaAat 2251.059 kb on - strand, within ygaAat 2251.186 kb on + strandat 2251.187 kb on - strandat 2251.192 kb on + strandat 2251.204 kb on + strandat 2251.205 kb on - strandat 2251.205 kb on - strandat 2251.205 kb on - strandat 2251.222 kb on + strandat 2251.231 kb on + strandat 2251.274 kb on + strandat 2251.275 kb on - strandat 2251.359 kb on + strandat 2251.404 kb on - strandat 2251.468 kb on - strand, within gshAat 2251.494 kb on + strand, within gshAat 2251.494 kb on + strand, within gshAat 2251.494 kb on + strand, within gshAat 2251.495 kb on - strand, within gshAat 2251.507 kb on - strand, within gshAat 2251.527 kb on + strand, within gshAat 2251.570 kb on - strand, within gshAat 2251.579 kb on + strand, within gshAat 2251.626 kb on + strand, within gshAat 2251.627 kb on - strand, within gshAat 2251.758 kb on + strand, within gshA

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
remove
2,249,514 + +0.6
2,249,514 + +0.9
2,249,690 + +0.9
2,249,917 + +0.9
2,249,917 + +0.7
2,249,962 + +0.0
2,249,962 + -0.3
2,249,965 + +0.0
2,250,156 - +0.4
2,250,165 + -0.9
2,250,200 + -0.6
2,250,201 - -0.1
2,250,220 - +1.9
2,250,286 - yqaB BNILDI_11150 0.16 +0.7
2,250,355 + yqaB BNILDI_11150 0.28 -0.2
2,250,439 + yqaB BNILDI_11150 0.43 +1.9
2,250,439 + yqaB BNILDI_11150 0.43 +0.5
2,250,659 - yqaB BNILDI_11150 0.81 +1.1
2,250,813 + ygaA BNILDI_11155 0.12 +0.9
2,250,875 - ygaA BNILDI_11155 0.27 -0.0
2,250,912 + ygaA BNILDI_11155 0.35 +1.9
2,250,913 - ygaA BNILDI_11155 0.36 -0.4
2,250,913 - ygaA BNILDI_11155 0.36 +0.6
2,250,931 - ygaA BNILDI_11155 0.40 +0.2
2,250,931 - ygaA BNILDI_11155 0.40 -0.7
2,250,964 - ygaA BNILDI_11155 0.48 -0.1
2,251,026 - ygaA BNILDI_11155 0.62 -0.5
2,251,029 - ygaA BNILDI_11155 0.63 +0.9
2,251,031 + ygaA BNILDI_11155 0.63 -1.8
2,251,058 + ygaA BNILDI_11155 0.69 +1.4
2,251,059 - ygaA BNILDI_11155 0.70 -0.3
2,251,186 + -0.2
2,251,187 - -0.3
2,251,192 + -1.4
2,251,204 + +0.3
2,251,205 - -1.1
2,251,205 - -1.0
2,251,205 - +0.5
2,251,222 + +0.9
2,251,231 + +0.0
2,251,274 + -0.7
2,251,275 - -0.3
2,251,359 + -1.0
2,251,404 - +0.2
2,251,468 - gshA BNILDI_11160 0.13 -0.4
2,251,494 + gshA BNILDI_11160 0.15 -0.4
2,251,494 + gshA BNILDI_11160 0.15 +0.9
2,251,494 + gshA BNILDI_11160 0.15 +1.9
2,251,495 - gshA BNILDI_11160 0.15 +0.3
2,251,507 - gshA BNILDI_11160 0.16 -0.9
2,251,527 + gshA BNILDI_11160 0.17 -0.1
2,251,570 - gshA BNILDI_11160 0.20 -1.5
2,251,579 + gshA BNILDI_11160 0.20 +1.1
2,251,626 + gshA BNILDI_11160 0.23 -1.0
2,251,627 - gshA BNILDI_11160 0.24 -0.7
2,251,758 + gshA BNILDI_11160 0.32 +0.1

Or see this region's nucleotide sequence