Experiment: MPec2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secA and mutT are separated by 59 nucleotides mutT and yacG are separated by 99 nucleotides yacG and zapD are separated by 9 nucleotides zapD and coaE overlap by 1 nucleotides coaE and yacM are separated by 24 nucleotides yacM and guaC are separated by 155 nucleotides
BNILDI_08675: secA - preprotein translocase subunit SecA, at 1,737,487 to 1,740,192
secA
BNILDI_08685: mutT - 8-oxo-dGTP diphosphatase MutT, at 1,740,252 to 1,740,641
mutT
BNILDI_08695: yacG - DNA gyrase inhibitor YacG, at 1,740,741 to 1,740,938
yacG
BNILDI_08700: zapD - cell division protein ZapD, at 1,740,948 to 1,741,691
zapD
BNILDI_08705: coaE - dephospho-CoA kinase, at 1,741,691 to 1,742,311
coaE
BNILDI_08710: yacM - Protein YacM, at 1,742,336 to 1,742,380
yacM
BNILDI_08715: guaC - GMP reductase, at 1,742,536 to 1,743,579
guaC
Position (kb)
1740
1741
1742 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1739.978 kb on + strand at 1739.978 kb on + strand at 1739.978 kb on + strand at 1739.978 kb on + strand at 1739.982 kb on + strand at 1739.983 kb on - strand at 1739.983 kb on - strand at 1740.057 kb on + strand at 1740.058 kb on - strand at 1740.190 kb on + strand at 1740.190 kb on + strand at 1740.278 kb on + strand at 1740.469 kb on - strand, within mutT at 1740.488 kb on + strand, within mutT at 1740.488 kb on + strand, within mutT at 1740.488 kb on + strand, within mutT at 1740.489 kb on - strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.490 kb on + strand, within mutT at 1740.491 kb on - strand, within mutT at 1740.572 kb on - strand, within mutT at 1740.581 kb on + strand, within mutT at 1740.581 kb on + strand, within mutT at 1740.629 kb on + strand at 1740.719 kb on + strand at 1740.720 kb on - strand at 1740.736 kb on - strand at 1740.926 kb on + strand at 1740.927 kb on - strand at 1741.037 kb on + strand, within zapD at 1741.037 kb on + strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.038 kb on - strand, within zapD at 1741.170 kb on + strand, within zapD at 1741.187 kb on + strand, within zapD at 1741.187 kb on + strand, within zapD at 1741.188 kb on - strand, within zapD at 1741.281 kb on - strand, within zapD at 1741.386 kb on + strand, within zapD at 1741.408 kb on - strand, within zapD at 1741.420 kb on - strand, within zapD at 1741.475 kb on + strand, within zapD at 1741.476 kb on - strand, within zapD at 1741.644 kb on - strand at 1741.660 kb on - strand at 1741.692 kb on + strand at 1742.300 kb on + strand at 1742.300 kb on + strand at 1742.301 kb on - strand at 1742.301 kb on - strand at 1742.301 kb on - strand at 1742.301 kb on - strand at 1742.312 kb on - strand at 1742.312 kb on - strand at 1742.402 kb on - strand at 1742.474 kb on + strand at 1742.474 kb on + strand at 1742.502 kb on + strand at 1742.508 kb on + strand at 1742.509 kb on - strand at 1742.509 kb on - strand at 1742.511 kb on - strand at 1742.511 kb on - strand at 1742.542 kb on - strand at 1742.568 kb on + strand at 1742.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MPec2 remove 1,739,978 + -0.8 1,739,978 + +1.2 1,739,978 + -0.3 1,739,978 + +0.4 1,739,982 + -0.3 1,739,983 - -0.3 1,739,983 - +0.4 1,740,057 + -0.1 1,740,058 - +2.0 1,740,190 + +0.1 1,740,190 + +1.4 1,740,278 + +0.1 1,740,469 - mutT BNILDI_08685 0.56 +0.2 1,740,488 + mutT BNILDI_08685 0.61 +1.5 1,740,488 + mutT BNILDI_08685 0.61 -0.8 1,740,488 + mutT BNILDI_08685 0.61 -0.6 1,740,489 - mutT BNILDI_08685 0.61 +0.2 1,740,490 + mutT BNILDI_08685 0.61 +0.3 1,740,490 + mutT BNILDI_08685 0.61 +1.2 1,740,490 + mutT BNILDI_08685 0.61 +0.0 1,740,490 + mutT BNILDI_08685 0.61 -0.5 1,740,490 + mutT BNILDI_08685 0.61 -0.2 1,740,490 + mutT BNILDI_08685 0.61 -0.8 1,740,490 + mutT BNILDI_08685 0.61 -0.2 1,740,491 - mutT BNILDI_08685 0.61 +0.0 1,740,572 - mutT BNILDI_08685 0.82 -1.2 1,740,581 + mutT BNILDI_08685 0.84 +0.2 1,740,581 + mutT BNILDI_08685 0.84 -0.6 1,740,629 + +0.9 1,740,719 + +0.9 1,740,720 - -0.5 1,740,736 - +0.4 1,740,926 + +0.4 1,740,927 - -1.3 1,741,037 + zapD BNILDI_08700 0.12 -0.7 1,741,037 + zapD BNILDI_08700 0.12 +0.1 1,741,038 - zapD BNILDI_08700 0.12 -0.5 1,741,038 - zapD BNILDI_08700 0.12 -0.6 1,741,038 - zapD BNILDI_08700 0.12 +0.3 1,741,038 - zapD BNILDI_08700 0.12 +0.4 1,741,038 - zapD BNILDI_08700 0.12 +0.1 1,741,038 - zapD BNILDI_08700 0.12 +0.3 1,741,170 + zapD BNILDI_08700 0.30 -0.6 1,741,187 + zapD BNILDI_08700 0.32 -3.2 1,741,187 + zapD BNILDI_08700 0.32 -0.1 1,741,188 - zapD BNILDI_08700 0.32 -0.8 1,741,281 - zapD BNILDI_08700 0.45 +0.1 1,741,386 + zapD BNILDI_08700 0.59 +0.8 1,741,408 - zapD BNILDI_08700 0.62 -0.5 1,741,420 - zapD BNILDI_08700 0.63 -0.0 1,741,475 + zapD BNILDI_08700 0.71 -0.6 1,741,476 - zapD BNILDI_08700 0.71 +1.4 1,741,644 - -0.5 1,741,660 - -0.0 1,741,692 + -0.3 1,742,300 + -2.4 1,742,300 + -2.1 1,742,301 - -0.3 1,742,301 - -0.0 1,742,301 - +0.2 1,742,301 - -2.7 1,742,312 - +0.7 1,742,312 - +0.0 1,742,402 - -0.6 1,742,474 + -0.3 1,742,474 + +2.0 1,742,502 + +1.0 1,742,508 + -0.1 1,742,509 - +0.6 1,742,509 - +0.0 1,742,511 - +0.2 1,742,511 - -0.0 1,742,542 - -0.1 1,742,568 + -1.4 1,742,605 - -1.6
Or see this region's nucleotide sequence