Strain Fitness in Escherichia coli ECRC62 around BNILDI_08665

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntftsZ and lpxC are separated by 100 nucleotideslpxC and secM are separated by 155 nucleotidessecM and secA are separated by 61 nucleotides BNILDI_08660: ftsZ - cell division protein FtsZ, at 1,734,513 to 1,735,664 ftsZ BNILDI_08665: lpxC - UDP-3-O-acyl-N-acetylglucosamine deacetylase, at 1,735,765 to 1,736,682 lpxC BNILDI_08670: secM - secA translation cis-regulator SecM, at 1,736,838 to 1,737,425 secM BNILDI_08675: secA - preprotein translocase subunit SecA, at 1,737,487 to 1,740,192 secA Position (kb) 1735 1736 1737Strain fitness (log2 ratio) -1 0 1 2at 1735.726 kb on + strandat 1735.726 kb on + strandat 1735.741 kb on + strandat 1735.745 kb on + strandat 1735.745 kb on + strandat 1735.745 kb on + strandat 1735.758 kb on + strandat 1736.690 kb on + strandat 1736.690 kb on + strandat 1736.690 kb on + strandat 1736.690 kb on + strandat 1736.690 kb on + strandat 1736.690 kb on + strandat 1736.690 kb on + strandat 1736.691 kb on - strandat 1736.692 kb on + strandat 1736.700 kb on + strandat 1736.769 kb on - strandat 1736.777 kb on + strandat 1736.827 kb on + strandat 1736.827 kb on + strandat 1736.858 kb on + strandat 1736.974 kb on + strand, within secMat 1737.423 kb on + strandat 1737.432 kb on + strandat 1737.453 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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1,735,726 + +0.4
1,735,726 + +0.7
1,735,741 + +2.0
1,735,745 + +2.3
1,735,745 + +0.0
1,735,745 + +0.0
1,735,758 + +1.3
1,736,690 + -0.6
1,736,690 + -0.2
1,736,690 + +0.4
1,736,690 + -0.9
1,736,690 + -0.3
1,736,690 + -0.6
1,736,690 + +0.2
1,736,691 - -0.0
1,736,692 + -0.1
1,736,700 + -0.5
1,736,769 - -1.0
1,736,777 + -0.0
1,736,827 + +1.2
1,736,827 + -0.0
1,736,858 + -0.9
1,736,974 + secM BNILDI_08670 0.23 -0.6
1,737,423 + -0.2
1,737,432 + -0.0
1,737,453 + +1.1

Or see this region's nucleotide sequence