Experiment: MPec2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kefC and BNILDI_08410 are separated by 80 nucleotides BNILDI_08410 and apaH are separated by 77 nucleotides apaH and apaG are separated by 6 nucleotides apaG and rsmA are separated by 2 nucleotides
BNILDI_08405: kefC - glutathione-regulated potassium-efflux system protein KefC, at 1,678,453 to 1,680,315
kefC
BNILDI_08410: BNILDI_08410 - Dihydrofolate reductase, at 1,680,396 to 1,680,986
_08410
BNILDI_08415: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 1,681,064 to 1,681,906
apaH
BNILDI_08420: apaG - Co2+/Mg2+ efflux protein ApaG, at 1,681,913 to 1,682,290
apaG
BNILDI_08425: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA, at 1,682,293 to 1,683,114
rsmA
Position (kb) 1681
1682 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1680.064 kb on + strand, within kefC at 1680.316 kb on + strand at 1680.414 kb on + strand at 1680.414 kb on + strand at 1680.467 kb on + strand, within BNILDI_08410 at 1680.467 kb on + strand, within BNILDI_08410 at 1680.472 kb on - strand, within BNILDI_08410 at 1680.473 kb on + strand, within BNILDI_08410 at 1681.049 kb on + strand at 1681.050 kb on - strand at 1681.050 kb on - strand at 1681.050 kb on - strand at 1681.050 kb on - strand at 1681.050 kb on - strand at 1681.065 kb on + strand at 1681.185 kb on + strand, within apaH at 1681.186 kb on - strand, within apaH at 1681.187 kb on + strand, within apaH at 1681.188 kb on - strand, within apaH at 1681.190 kb on - strand, within apaH at 1681.246 kb on - strand, within apaH at 1681.323 kb on + strand, within apaH at 1681.323 kb on + strand, within apaH at 1681.323 kb on + strand, within apaH at 1681.323 kb on + strand, within apaH at 1681.433 kb on - strand, within apaH at 1681.445 kb on - strand, within apaH at 1681.609 kb on + strand, within apaH at 1681.667 kb on - strand, within apaH at 1681.730 kb on + strand, within apaH at 1681.731 kb on - strand, within apaH at 1681.831 kb on + strand at 1681.844 kb on - strand at 1681.870 kb on - strand at 1681.896 kb on + strand at 1681.896 kb on + strand at 1681.897 kb on - strand at 1681.897 kb on - strand at 1681.907 kb on - strand at 1681.914 kb on + strand at 1682.047 kb on - strand, within apaG at 1682.197 kb on + strand, within apaG at 1682.266 kb on + strand at 1682.295 kb on - strand at 1682.416 kb on - strand, within rsmA at 1682.636 kb on - strand, within rsmA at 1682.636 kb on - strand, within rsmA at 1682.768 kb on + strand, within rsmA
Per-strain Table
Position Strand Gene LocusTag Fraction MPec2 remove 1,680,064 + kefC BNILDI_08405 0.86 -0.0 1,680,316 + -2.3 1,680,414 + +0.6 1,680,414 + -0.8 1,680,467 + BNILDI_08410 0.12 +1.3 1,680,467 + BNILDI_08410 0.12 -0.1 1,680,472 - BNILDI_08410 0.13 -1.0 1,680,473 + BNILDI_08410 0.13 +0.1 1,681,049 + -0.7 1,681,050 - -0.5 1,681,050 - -1.3 1,681,050 - -1.0 1,681,050 - -0.5 1,681,050 - +1.1 1,681,065 + -0.5 1,681,185 + apaH BNILDI_08415 0.14 -1.3 1,681,186 - apaH BNILDI_08415 0.14 +1.0 1,681,187 + apaH BNILDI_08415 0.15 -1.1 1,681,188 - apaH BNILDI_08415 0.15 +1.0 1,681,190 - apaH BNILDI_08415 0.15 +1.0 1,681,246 - apaH BNILDI_08415 0.22 -2.3 1,681,323 + apaH BNILDI_08415 0.31 +0.4 1,681,323 + apaH BNILDI_08415 0.31 +1.0 1,681,323 + apaH BNILDI_08415 0.31 +1.0 1,681,323 + apaH BNILDI_08415 0.31 -0.6 1,681,433 - apaH BNILDI_08415 0.44 -0.9 1,681,445 - apaH BNILDI_08415 0.45 +2.7 1,681,609 + apaH BNILDI_08415 0.65 +1.3 1,681,667 - apaH BNILDI_08415 0.72 +1.0 1,681,730 + apaH BNILDI_08415 0.79 -0.8 1,681,731 - apaH BNILDI_08415 0.79 -2.4 1,681,831 + +1.0 1,681,844 - -0.8 1,681,870 - -0.0 1,681,896 + +1.0 1,681,896 + -0.0 1,681,897 - +1.0 1,681,897 - +1.0 1,681,907 - +0.6 1,681,914 + -0.8 1,682,047 - apaG BNILDI_08420 0.35 +0.2 1,682,197 + apaG BNILDI_08420 0.75 +1.0 1,682,266 + +0.2 1,682,295 - -0.0 1,682,416 - rsmA BNILDI_08425 0.15 -0.0 1,682,636 - rsmA BNILDI_08425 0.42 -1.3 1,682,636 - rsmA BNILDI_08425 0.42 -0.8 1,682,768 + rsmA BNILDI_08425 0.58 +1.0
Or see this region's nucleotide sequence