Strain Fitness in Escherichia coli ECRC62 around BNILDI_06730

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdcuS and yjdI are separated by 180 nucleotidesyjdI and yjdJ are separated by 11 nucleotidesyjdJ and ghoS are separated by 226 nucleotidesghoS and ghoT are separated by 27 nucleotidesghoT and lysS are separated by 118 nucleotides BNILDI_06715: dcuS - Sensor histidine kinase DcuS, at 1,344,054 to 1,345,685 dcuS BNILDI_06720: yjdI - Uncharacterized protein YjdI, at 1,345,866 to 1,346,096 yjdI BNILDI_06725: yjdJ - Uncharacterized protein YjdJ, at 1,346,108 to 1,346,380 yjdJ BNILDI_06730: ghoS - type V toxin-antitoxin system endoribonuclease antitoxin GhoS, at 1,346,607 to 1,346,903 ghoS BNILDI_06735: ghoT - type V toxin-antitoxin system toxin GhoT, at 1,346,931 to 1,347,104 ghoT BNILDI_06740: lysS - lysine--tRNA ligase, at 1,347,223 to 1,348,740 lysS Position (kb) 1346 1347Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1345.617 kb on + strandat 1345.632 kb on - strandat 1345.632 kb on - strandat 1345.764 kb on + strandat 1345.764 kb on + strandat 1345.767 kb on - strandat 1345.768 kb on + strandat 1345.769 kb on - strandat 1345.769 kb on - strandat 1345.769 kb on - strandat 1345.808 kb on - strandat 1345.831 kb on - strandat 1345.831 kb on - strandat 1345.844 kb on + strandat 1345.967 kb on - strand, within yjdIat 1345.988 kb on + strand, within yjdIat 1346.058 kb on - strand, within yjdIat 1346.082 kb on + strandat 1346.219 kb on - strand, within yjdJat 1346.312 kb on - strand, within yjdJat 1346.319 kb on + strand, within yjdJat 1346.319 kb on + strand, within yjdJat 1346.360 kb on + strandat 1346.361 kb on - strandat 1346.535 kb on - strandat 1346.603 kb on + strandat 1346.700 kb on + strand, within ghoSat 1346.701 kb on - strand, within ghoSat 1346.701 kb on - strand, within ghoSat 1346.822 kb on + strand, within ghoSat 1346.865 kb on - strand, within ghoSat 1346.980 kb on + strand, within ghoTat 1346.981 kb on - strand, within ghoTat 1346.994 kb on - strand, within ghoTat 1347.023 kb on + strand, within ghoTat 1347.111 kb on + strandat 1347.111 kb on + strandat 1347.112 kb on - strandat 1347.213 kb on - strandat 1347.256 kb on - strandat 1347.256 kb on - strandat 1347.268 kb on - strandat 1347.271 kb on - strandat 1347.271 kb on - strandat 1347.271 kb on - strandat 1347.271 kb on - strandat 1347.292 kb on - strandat 1347.313 kb on - strandat 1347.359 kb on + strandat 1347.359 kb on + strandat 1347.372 kb on - strandat 1347.573 kb on + strand, within lysSat 1347.630 kb on + strand, within lysSat 1347.631 kb on - strand, within lysSat 1347.631 kb on - strand, within lysSat 1347.631 kb on - strand, within lysSat 1347.631 kb on - strand, within lysSat 1347.660 kb on + strand, within lysSat 1347.680 kb on - strand, within lysSat 1347.734 kb on + strand, within lysSat 1347.813 kb on + strand, within lysSat 1347.890 kb on + strand, within lysSat 1347.900 kb on - strand, within lysS

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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1,345,617 + +2.1
1,345,632 - +0.5
1,345,632 - -0.7
1,345,764 + -0.0
1,345,764 + +0.8
1,345,767 - -0.2
1,345,768 + -0.2
1,345,769 - -0.5
1,345,769 - +3.0
1,345,769 - +0.5
1,345,808 - -0.3
1,345,831 - +0.3
1,345,831 - -0.4
1,345,844 + -0.5
1,345,967 - yjdI BNILDI_06720 0.44 -0.7
1,345,988 + yjdI BNILDI_06720 0.53 -0.4
1,346,058 - yjdI BNILDI_06720 0.83 -0.8
1,346,082 + -2.7
1,346,219 - yjdJ BNILDI_06725 0.41 +1.0
1,346,312 - yjdJ BNILDI_06725 0.75 +0.3
1,346,319 + yjdJ BNILDI_06725 0.77 +2.0
1,346,319 + yjdJ BNILDI_06725 0.77 +1.0
1,346,360 + -0.5
1,346,361 - -0.6
1,346,535 - -0.7
1,346,603 + -0.6
1,346,700 + ghoS BNILDI_06730 0.31 -1.6
1,346,701 - ghoS BNILDI_06730 0.32 -0.7
1,346,701 - ghoS BNILDI_06730 0.32 +1.3
1,346,822 + ghoS BNILDI_06730 0.72 -0.4
1,346,865 - ghoS BNILDI_06730 0.87 -0.1
1,346,980 + ghoT BNILDI_06735 0.28 +2.0
1,346,981 - ghoT BNILDI_06735 0.29 -0.4
1,346,994 - ghoT BNILDI_06735 0.36 -0.3
1,347,023 + ghoT BNILDI_06735 0.53 +0.1
1,347,111 + +0.1
1,347,111 + +0.4
1,347,112 - -0.3
1,347,213 - -0.6
1,347,256 - +1.0
1,347,256 - +1.0
1,347,268 - +1.3
1,347,271 - -0.1
1,347,271 - -0.0
1,347,271 - -0.1
1,347,271 - -1.1
1,347,292 - -0.4
1,347,313 - -0.8
1,347,359 + -0.5
1,347,359 + -0.2
1,347,372 - +0.3
1,347,573 + lysS BNILDI_06740 0.23 +0.0
1,347,630 + lysS BNILDI_06740 0.27 -0.8
1,347,631 - lysS BNILDI_06740 0.27 +1.4
1,347,631 - lysS BNILDI_06740 0.27 +0.4
1,347,631 - lysS BNILDI_06740 0.27 +1.1
1,347,631 - lysS BNILDI_06740 0.27 +0.3
1,347,660 + lysS BNILDI_06740 0.29 -0.4
1,347,680 - lysS BNILDI_06740 0.30 +1.0
1,347,734 + lysS BNILDI_06740 0.34 -0.2
1,347,813 + lysS BNILDI_06740 0.39 +0.8
1,347,890 + lysS BNILDI_06740 0.44 +0.7
1,347,900 - lysS BNILDI_06740 0.45 +1.4

Or see this region's nucleotide sequence