Strain Fitness in Escherichia coli ECRC62 around BNILDI_22200

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntArg_trna and fimZ are separated by 242 nucleotidesfimZ and sfmF are separated by 2 nucleotidessfmF and fimH are separated by 10 nucleotides BNILDI_22190: Arg_trna - tRNA-Arg(tct), at 4,459,485 to 4,459,561 _trna BNILDI_22195: fimZ - fimbria biosynthesis transcriptional regulator FimZ, at 4,459,804 to 4,460,436 fimZ BNILDI_22200: sfmF - fimbria assembly protein, at 4,460,439 to 4,460,954 sfmF BNILDI_22205: fimH - type 1 fimbria D-mannose specific adhesin FimH, at 4,460,965 to 4,461,972 fimH Position (kb) 4460 4461Strain fitness (log2 ratio) -1 0 1 2at 4459.583 kb on - strandat 4459.735 kb on - strandat 4459.828 kb on - strandat 4459.833 kb on + strandat 4459.834 kb on - strandat 4459.842 kb on + strandat 4459.843 kb on - strandat 4460.075 kb on - strand, within fimZat 4460.090 kb on + strand, within fimZat 4460.553 kb on + strand, within sfmFat 4460.604 kb on - strand, within sfmFat 4460.604 kb on - strand, within sfmFat 4460.604 kb on - strand, within sfmFat 4460.604 kb on - strand, within sfmFat 4460.604 kb on - strand, within sfmFat 4460.607 kb on - strand, within sfmFat 4460.607 kb on - strand, within sfmFat 4460.607 kb on - strand, within sfmFat 4460.611 kb on - strand, within sfmFat 4460.691 kb on + strand, within sfmFat 4460.736 kb on - strand, within sfmFat 4460.772 kb on + strand, within sfmFat 4460.784 kb on - strand, within sfmFat 4460.811 kb on - strand, within sfmFat 4460.830 kb on - strand, within sfmFat 4460.874 kb on - strand, within sfmFat 4460.987 kb on + strandat 4461.103 kb on - strand, within fimHat 4461.224 kb on + strand, within fimHat 4461.224 kb on + strand, within fimHat 4461.412 kb on - strand, within fimHat 4461.419 kb on + strand, within fimHat 4461.462 kb on - strand, within fimHat 4461.661 kb on + strand, within fimHat 4461.906 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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4,459,583 - -0.8
4,459,735 - +0.2
4,459,828 - +1.1
4,459,833 + -0.6
4,459,834 - +0.8
4,459,842 + -0.8
4,459,843 - -1.5
4,460,075 - fimZ BNILDI_22195 0.43 +0.0
4,460,090 + fimZ BNILDI_22195 0.45 +2.1
4,460,553 + sfmF BNILDI_22200 0.22 +0.8
4,460,604 - sfmF BNILDI_22200 0.32 -0.1
4,460,604 - sfmF BNILDI_22200 0.32 -0.3
4,460,604 - sfmF BNILDI_22200 0.32 +0.5
4,460,604 - sfmF BNILDI_22200 0.32 +0.9
4,460,604 - sfmF BNILDI_22200 0.32 -0.6
4,460,607 - sfmF BNILDI_22200 0.33 -0.3
4,460,607 - sfmF BNILDI_22200 0.33 +1.2
4,460,607 - sfmF BNILDI_22200 0.33 -0.1
4,460,611 - sfmF BNILDI_22200 0.33 +2.4
4,460,691 + sfmF BNILDI_22200 0.49 +0.4
4,460,736 - sfmF BNILDI_22200 0.58 -0.9
4,460,772 + sfmF BNILDI_22200 0.65 -0.5
4,460,784 - sfmF BNILDI_22200 0.67 +0.4
4,460,811 - sfmF BNILDI_22200 0.72 +1.1
4,460,830 - sfmF BNILDI_22200 0.76 -0.3
4,460,874 - sfmF BNILDI_22200 0.84 +1.6
4,460,987 + +2.6
4,461,103 - fimH BNILDI_22205 0.14 +0.8
4,461,224 + fimH BNILDI_22205 0.26 +0.1
4,461,224 + fimH BNILDI_22205 0.26 -0.0
4,461,412 - fimH BNILDI_22205 0.44 +1.3
4,461,419 + fimH BNILDI_22205 0.45 +0.1
4,461,462 - fimH BNILDI_22205 0.49 -1.5
4,461,661 + fimH BNILDI_22205 0.69 +0.8
4,461,906 + +0.4

Or see this region's nucleotide sequence