Strain Fitness in Escherichia coli ECRC62 around BNILDI_20600

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrimK and nfsA are separated by 60 nucleotidesnfsA and BNILDI_20605 overlap by 4 nucleotidesBNILDI_20605 and ybjC overlap by 17 nucleotidesybjC and grxA are separated by 159 nucleotides BNILDI_20595: rimK - 30S ribosomal protein S6--L-glutamate ligase, at 4,148,895 to 4,149,797 rimK BNILDI_20600: nfsA - oxygen-insensitive NADPH nitroreductase, at 4,149,858 to 4,150,253 nfsA BNILDI_20605: BNILDI_20605 - Nitroreductase NfsA, at 4,150,250 to 4,150,579 _20605 BNILDI_20610: ybjC - Uncharacterized protein YbjC, at 4,150,563 to 4,150,850 ybjC BNILDI_20615: grxA - glutaredoxin 1, at 4,151,010 to 4,151,267 grxA Position (kb) 4149 4150 4151Strain fitness (log2 ratio) -2 -1 0 1 2at 4148.881 kb on + strandat 4149.263 kb on - strand, within rimKat 4149.279 kb on - strand, within rimKat 4149.542 kb on - strand, within rimKat 4149.584 kb on - strand, within rimKat 4149.650 kb on - strand, within rimKat 4149.676 kb on - strand, within rimKat 4149.676 kb on - strand, within rimKat 4149.686 kb on - strand, within rimKat 4149.686 kb on - strand, within rimKat 4149.752 kb on + strandat 4149.761 kb on - strandat 4149.784 kb on - strandat 4149.840 kb on + strandat 4149.841 kb on - strandat 4149.936 kb on - strand, within nfsAat 4150.006 kb on + strand, within nfsAat 4150.040 kb on - strand, within nfsAat 4150.198 kb on + strand, within nfsAat 4150.198 kb on + strand, within nfsAat 4150.199 kb on - strand, within nfsAat 4150.199 kb on - strand, within nfsAat 4150.200 kb on + strand, within nfsAat 4150.412 kb on + strand, within BNILDI_20605at 4150.558 kb on + strandat 4150.559 kb on - strandat 4150.607 kb on - strand, within ybjCat 4150.813 kb on - strand, within ybjCat 4150.851 kb on - strandat 4150.882 kb on + strandat 4150.897 kb on + strandat 4150.981 kb on - strandat 4150.988 kb on + strandat 4151.009 kb on - strandat 4151.112 kb on + strand, within grxAat 4151.112 kb on + strand, within grxAat 4151.113 kb on - strand, within grxAat 4151.116 kb on + strand, within grxAat 4151.117 kb on - strand, within grxAat 4151.120 kb on + strand, within grxAat 4151.121 kb on - strand, within grxAat 4151.121 kb on - strand, within grxAat 4151.152 kb on + strand, within grxA

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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4,148,881 + -0.4
4,149,263 - rimK BNILDI_20595 0.41 -0.8
4,149,279 - rimK BNILDI_20595 0.43 -0.9
4,149,542 - rimK BNILDI_20595 0.72 -1.2
4,149,584 - rimK BNILDI_20595 0.76 +1.1
4,149,650 - rimK BNILDI_20595 0.84 -0.8
4,149,676 - rimK BNILDI_20595 0.86 +0.5
4,149,676 - rimK BNILDI_20595 0.86 +0.0
4,149,686 - rimK BNILDI_20595 0.88 -0.7
4,149,686 - rimK BNILDI_20595 0.88 -0.4
4,149,752 + +0.6
4,149,761 - +0.9
4,149,784 - -0.3
4,149,840 + +1.1
4,149,841 - +0.6
4,149,936 - nfsA BNILDI_20600 0.20 +1.9
4,150,006 + nfsA BNILDI_20600 0.37 -0.3
4,150,040 - nfsA BNILDI_20600 0.46 -2.1
4,150,198 + nfsA BNILDI_20600 0.86 -0.6
4,150,198 + nfsA BNILDI_20600 0.86 +1.4
4,150,199 - nfsA BNILDI_20600 0.86 +0.5
4,150,199 - nfsA BNILDI_20600 0.86 +0.9
4,150,200 + nfsA BNILDI_20600 0.86 +0.3
4,150,412 + BNILDI_20605 0.49 -1.2
4,150,558 + +0.7
4,150,559 - -0.4
4,150,607 - ybjC BNILDI_20610 0.15 +0.5
4,150,813 - ybjC BNILDI_20610 0.87 +1.1
4,150,851 - -0.4
4,150,882 + -0.1
4,150,897 + +0.5
4,150,981 - +1.9
4,150,988 + +2.5
4,151,009 - -0.5
4,151,112 + grxA BNILDI_20615 0.40 -0.6
4,151,112 + grxA BNILDI_20615 0.40 +1.3
4,151,113 - grxA BNILDI_20615 0.40 +0.1
4,151,116 + grxA BNILDI_20615 0.41 +1.1
4,151,117 - grxA BNILDI_20615 0.41 +0.4
4,151,120 + grxA BNILDI_20615 0.43 -0.2
4,151,121 - grxA BNILDI_20615 0.43 +0.0
4,151,121 - grxA BNILDI_20615 0.43 +0.2
4,151,152 + grxA BNILDI_20615 0.55 -0.1

Or see this region's nucleotide sequence