Experiment: MPec2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt puuB and puuC are separated by 1 nucleotides puuC and puuR are separated by 274 nucleotides puuR and puuD are separated by 26 nucleotides
BNILDI_18260: puuB - gamma-glutamylputrescine oxidase, at 3,677,318 to 3,678,598
puuB
BNILDI_18265: puuC - aldehyde dehydrogenase PuuC, at 3,678,600 to 3,680,087
puuC
BNILDI_18270: puuR - HTH-type transcriptional regulator PuuR, at 3,680,362 to 3,680,919
puuR
BNILDI_18275: puuD - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 3,680,946 to 3,681,698
puuD
Position (kb)
3678
3679
3680
3681 Strain fitness (log2 ratio)
-1
0
1
2 at 3677.752 kb on - strand, within puuB at 3677.755 kb on + strand, within puuB at 3677.772 kb on + strand, within puuB at 3677.873 kb on + strand, within puuB at 3677.890 kb on - strand, within puuB at 3677.914 kb on - strand, within puuB at 3677.914 kb on - strand, within puuB at 3677.914 kb on - strand, within puuB at 3677.994 kb on + strand, within puuB at 3678.058 kb on + strand, within puuB at 3678.093 kb on + strand, within puuB at 3678.094 kb on - strand, within puuB at 3678.441 kb on - strand, within puuB at 3678.490 kb on - strand at 3678.552 kb on + strand at 3678.574 kb on - strand at 3678.576 kb on + strand at 3678.586 kb on + strand at 3678.586 kb on + strand at 3678.689 kb on + strand at 3678.691 kb on + strand at 3678.728 kb on - strand at 3678.728 kb on - strand at 3678.843 kb on + strand, within puuC at 3678.844 kb on - strand, within puuC at 3679.020 kb on - strand, within puuC at 3679.025 kb on + strand, within puuC at 3679.057 kb on - strand, within puuC at 3679.201 kb on + strand, within puuC at 3679.202 kb on - strand, within puuC at 3679.672 kb on + strand, within puuC at 3679.673 kb on - strand, within puuC at 3679.839 kb on - strand, within puuC at 3679.839 kb on - strand, within puuC at 3680.055 kb on - strand at 3680.115 kb on + strand at 3680.116 kb on - strand at 3680.116 kb on - strand at 3680.117 kb on + strand at 3680.117 kb on + strand at 3680.117 kb on + strand at 3680.117 kb on + strand at 3680.118 kb on - strand at 3680.169 kb on + strand at 3680.188 kb on + strand at 3680.192 kb on + strand at 3680.192 kb on + strand at 3680.192 kb on + strand at 3680.193 kb on - strand at 3680.249 kb on + strand at 3680.257 kb on + strand at 3680.314 kb on - strand at 3680.315 kb on + strand at 3680.315 kb on + strand at 3680.315 kb on + strand at 3680.347 kb on + strand at 3680.415 kb on + strand at 3680.445 kb on + strand, within puuR at 3680.454 kb on - strand, within puuR at 3680.527 kb on + strand, within puuR at 3680.548 kb on - strand, within puuR at 3680.548 kb on - strand, within puuR at 3680.665 kb on + strand, within puuR at 3680.665 kb on + strand, within puuR at 3680.665 kb on + strand, within puuR at 3680.744 kb on + strand, within puuR at 3680.744 kb on + strand, within puuR at 3680.744 kb on + strand, within puuR at 3680.744 kb on + strand, within puuR at 3680.745 kb on - strand, within puuR at 3680.745 kb on - strand, within puuR at 3680.745 kb on - strand, within puuR at 3680.745 kb on - strand, within puuR at 3680.812 kb on - strand, within puuR at 3680.920 kb on - strand at 3680.940 kb on + strand at 3680.944 kb on - strand at 3680.970 kb on - strand at 3680.991 kb on - strand at 3681.008 kb on + strand at 3681.054 kb on + strand, within puuD
Per-strain Table
Position Strand Gene LocusTag Fraction MPec2 remove 3,677,752 - puuB BNILDI_18260 0.34 -1.7 3,677,755 + puuB BNILDI_18260 0.34 +0.8 3,677,772 + puuB BNILDI_18260 0.35 +0.2 3,677,873 + puuB BNILDI_18260 0.43 +0.3 3,677,890 - puuB BNILDI_18260 0.45 -0.2 3,677,914 - puuB BNILDI_18260 0.47 -0.3 3,677,914 - puuB BNILDI_18260 0.47 -0.5 3,677,914 - puuB BNILDI_18260 0.47 -0.0 3,677,994 + puuB BNILDI_18260 0.53 -0.5 3,678,058 + puuB BNILDI_18260 0.58 +2.4 3,678,093 + puuB BNILDI_18260 0.60 +0.1 3,678,094 - puuB BNILDI_18260 0.61 +0.2 3,678,441 - puuB BNILDI_18260 0.88 +0.3 3,678,490 - -1.2 3,678,552 + +0.5 3,678,574 - +0.1 3,678,576 + +0.2 3,678,586 + +0.2 3,678,586 + +1.1 3,678,689 + -0.6 3,678,691 + +0.9 3,678,728 - -0.0 3,678,728 - +0.4 3,678,843 + puuC BNILDI_18265 0.16 -0.7 3,678,844 - puuC BNILDI_18265 0.16 -1.0 3,679,020 - puuC BNILDI_18265 0.28 +1.4 3,679,025 + puuC BNILDI_18265 0.29 -0.5 3,679,057 - puuC BNILDI_18265 0.31 +0.8 3,679,201 + puuC BNILDI_18265 0.40 -1.0 3,679,202 - puuC BNILDI_18265 0.40 +0.0 3,679,672 + puuC BNILDI_18265 0.72 -0.2 3,679,673 - puuC BNILDI_18265 0.72 -0.3 3,679,839 - puuC BNILDI_18265 0.83 +0.5 3,679,839 - puuC BNILDI_18265 0.83 -1.6 3,680,055 - -1.4 3,680,115 + +0.2 3,680,116 - +0.4 3,680,116 - +0.8 3,680,117 + +0.4 3,680,117 + +0.6 3,680,117 + -0.2 3,680,117 + -0.0 3,680,118 - +0.2 3,680,169 + +0.5 3,680,188 + +0.2 3,680,192 + -0.0 3,680,192 + +0.6 3,680,192 + +1.8 3,680,193 - +0.1 3,680,249 + -0.5 3,680,257 + +0.2 3,680,314 - +0.4 3,680,315 + +2.4 3,680,315 + -0.2 3,680,315 + +1.0 3,680,347 + +0.0 3,680,415 + -0.5 3,680,445 + puuR BNILDI_18270 0.15 +1.1 3,680,454 - puuR BNILDI_18270 0.16 -1.7 3,680,527 + puuR BNILDI_18270 0.30 -0.2 3,680,548 - puuR BNILDI_18270 0.33 -0.2 3,680,548 - puuR BNILDI_18270 0.33 -0.1 3,680,665 + puuR BNILDI_18270 0.54 -0.6 3,680,665 + puuR BNILDI_18270 0.54 +0.2 3,680,665 + puuR BNILDI_18270 0.54 +0.9 3,680,744 + puuR BNILDI_18270 0.68 +0.8 3,680,744 + puuR BNILDI_18270 0.68 -0.5 3,680,744 + puuR BNILDI_18270 0.68 +0.1 3,680,744 + puuR BNILDI_18270 0.68 -0.4 3,680,745 - puuR BNILDI_18270 0.69 -0.4 3,680,745 - puuR BNILDI_18270 0.69 +0.5 3,680,745 - puuR BNILDI_18270 0.69 -0.7 3,680,745 - puuR BNILDI_18270 0.69 -0.8 3,680,812 - puuR BNILDI_18270 0.81 +0.4 3,680,920 - +0.8 3,680,940 + -0.9 3,680,944 - -0.8 3,680,970 - +0.5 3,680,991 - +0.1 3,681,008 + -0.7 3,681,054 + puuD BNILDI_18275 0.14 +0.2
Or see this region's nucleotide sequence