Strain Fitness in Escherichia coli ECRC62 around BNILDI_12250

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttnp-IS3 and tnp-IS3 overlap by 4 nucleotidestnp-IS3 and yfgG are separated by 46 nucleotidesyfgG and BNILDI_12245 are separated by 35 nucleotidesBNILDI_12245 and pdeF are separated by 181 nucleotidespdeF and ppx are separated by 38 nucleotides BNILDI_12230: tnp-IS3 - IS3 family transposase, at 2,455,671 to 2,456,537 tnp-IS3 BNILDI_12235: tnp-IS3 - IS3 family IS3 transposase, at 2,456,534 to 2,456,833 tnp-IS3 BNILDI_12240: yfgG - Uncharacterized protein YfgG, at 2,456,880 to 2,457,038 yfgG BNILDI_12245: BNILDI_12245 - hypothetical protein, at 2,457,074 to 2,457,226 _12245 BNILDI_12250: pdeF - cyclic-guanylate-specific phosphodiesterase PdeF, at 2,457,408 to 2,459,651 pdeF BNILDI_12255: ppx - exopolyphosphatase, at 2,459,690 to 2,461,231 ppx Position (kb) 2457 2458 2459 2460Strain fitness (log2 ratio) -2 -1 0 1 2at 2456.979 kb on - strand, within yfgGat 2457.073 kb on + strandat 2457.098 kb on + strand, within BNILDI_12245at 2457.164 kb on + strand, within BNILDI_12245at 2457.190 kb on - strand, within BNILDI_12245at 2457.224 kb on + strandat 2457.233 kb on - strandat 2457.273 kb on + strandat 2457.320 kb on + strandat 2457.369 kb on - strandat 2457.407 kb on + strandat 2457.408 kb on - strandat 2457.427 kb on - strandat 2457.504 kb on - strandat 2457.546 kb on + strandat 2457.726 kb on - strand, within pdeFat 2457.737 kb on + strand, within pdeFat 2457.781 kb on + strand, within pdeFat 2457.781 kb on + strand, within pdeFat 2457.794 kb on - strand, within pdeFat 2457.844 kb on + strand, within pdeFat 2457.912 kb on + strand, within pdeFat 2458.084 kb on + strand, within pdeFat 2458.084 kb on + strand, within pdeFat 2458.141 kb on - strand, within pdeFat 2458.142 kb on + strand, within pdeFat 2458.142 kb on + strand, within pdeFat 2458.196 kb on + strand, within pdeFat 2458.197 kb on - strand, within pdeFat 2458.197 kb on - strand, within pdeFat 2458.279 kb on - strand, within pdeFat 2458.279 kb on - strand, within pdeFat 2458.340 kb on + strand, within pdeFat 2458.398 kb on + strand, within pdeFat 2458.398 kb on + strand, within pdeFat 2458.398 kb on + strand, within pdeFat 2458.479 kb on + strand, within pdeFat 2458.483 kb on + strand, within pdeFat 2458.483 kb on + strand, within pdeFat 2458.483 kb on + strand, within pdeFat 2458.483 kb on + strand, within pdeFat 2458.484 kb on - strand, within pdeFat 2458.519 kb on - strand, within pdeFat 2458.542 kb on + strand, within pdeFat 2458.600 kb on - strand, within pdeFat 2458.637 kb on + strand, within pdeFat 2458.746 kb on + strand, within pdeFat 2458.747 kb on - strand, within pdeFat 2458.747 kb on - strand, within pdeFat 2458.774 kb on - strand, within pdeFat 2458.774 kb on - strand, within pdeFat 2458.797 kb on - strand, within pdeFat 2458.838 kb on + strand, within pdeFat 2458.961 kb on + strand, within pdeFat 2458.977 kb on + strand, within pdeFat 2459.099 kb on + strand, within pdeFat 2459.099 kb on + strand, within pdeFat 2459.099 kb on + strand, within pdeFat 2459.100 kb on - strand, within pdeFat 2459.100 kb on - strand, within pdeFat 2459.100 kb on - strand, within pdeFat 2459.100 kb on - strand, within pdeFat 2459.100 kb on - strand, within pdeFat 2459.153 kb on + strand, within pdeFat 2459.153 kb on + strand, within pdeFat 2459.157 kb on - strand, within pdeFat 2459.220 kb on - strand, within pdeFat 2459.246 kb on + strand, within pdeFat 2459.433 kb on - strandat 2459.462 kb on - strandat 2459.517 kb on + strandat 2459.518 kb on - strandat 2459.518 kb on - strandat 2459.569 kb on - strandat 2459.570 kb on + strandat 2459.570 kb on + strandat 2459.591 kb on + strandat 2459.758 kb on - strandat 2459.804 kb on - strandat 2459.900 kb on + strand, within ppxat 2459.901 kb on - strand, within ppxat 2459.903 kb on + strand, within ppxat 2459.918 kb on + strand, within ppxat 2459.925 kb on - strand, within ppxat 2459.951 kb on - strand, within ppxat 2459.959 kb on + strand, within ppxat 2459.959 kb on + strand, within ppxat 2460.072 kb on + strand, within ppxat 2460.202 kb on - strand, within ppxat 2460.325 kb on - strand, within ppxat 2460.342 kb on - strand, within ppxat 2460.377 kb on + strand, within ppx

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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2,456,979 - yfgG BNILDI_12240 0.62 +0.2
2,457,073 + -0.1
2,457,098 + BNILDI_12245 0.16 -0.0
2,457,164 + BNILDI_12245 0.59 -1.0
2,457,190 - BNILDI_12245 0.76 +0.8
2,457,224 + -0.6
2,457,233 - +1.9
2,457,273 + -1.2
2,457,320 + -0.5
2,457,369 - +0.1
2,457,407 + +0.2
2,457,408 - +0.5
2,457,427 - +0.0
2,457,504 - -1.2
2,457,546 + +1.9
2,457,726 - pdeF BNILDI_12250 0.14 -0.4
2,457,737 + pdeF BNILDI_12250 0.15 -0.1
2,457,781 + pdeF BNILDI_12250 0.17 +1.9
2,457,781 + pdeF BNILDI_12250 0.17 -0.4
2,457,794 - pdeF BNILDI_12250 0.17 +0.2
2,457,844 + pdeF BNILDI_12250 0.19 +0.8
2,457,912 + pdeF BNILDI_12250 0.22 +0.1
2,458,084 + pdeF BNILDI_12250 0.30 -0.5
2,458,084 + pdeF BNILDI_12250 0.30 +0.3
2,458,141 - pdeF BNILDI_12250 0.33 +0.3
2,458,142 + pdeF BNILDI_12250 0.33 +0.5
2,458,142 + pdeF BNILDI_12250 0.33 +0.3
2,458,196 + pdeF BNILDI_12250 0.35 -1.0
2,458,197 - pdeF BNILDI_12250 0.35 -0.1
2,458,197 - pdeF BNILDI_12250 0.35 -1.5
2,458,279 - pdeF BNILDI_12250 0.39 +0.3
2,458,279 - pdeF BNILDI_12250 0.39 -0.1
2,458,340 + pdeF BNILDI_12250 0.42 +0.3
2,458,398 + pdeF BNILDI_12250 0.44 +1.5
2,458,398 + pdeF BNILDI_12250 0.44 -0.3
2,458,398 + pdeF BNILDI_12250 0.44 -0.7
2,458,479 + pdeF BNILDI_12250 0.48 -0.3
2,458,483 + pdeF BNILDI_12250 0.48 +0.2
2,458,483 + pdeF BNILDI_12250 0.48 -0.1
2,458,483 + pdeF BNILDI_12250 0.48 +1.7
2,458,483 + pdeF BNILDI_12250 0.48 -1.8
2,458,484 - pdeF BNILDI_12250 0.48 +0.3
2,458,519 - pdeF BNILDI_12250 0.50 -0.6
2,458,542 + pdeF BNILDI_12250 0.51 -0.1
2,458,600 - pdeF BNILDI_12250 0.53 +1.3
2,458,637 + pdeF BNILDI_12250 0.55 -0.0
2,458,746 + pdeF BNILDI_12250 0.60 +0.1
2,458,747 - pdeF BNILDI_12250 0.60 +0.6
2,458,747 - pdeF BNILDI_12250 0.60 +0.9
2,458,774 - pdeF BNILDI_12250 0.61 -0.1
2,458,774 - pdeF BNILDI_12250 0.61 +0.5
2,458,797 - pdeF BNILDI_12250 0.62 +0.6
2,458,838 + pdeF BNILDI_12250 0.64 +0.1
2,458,961 + pdeF BNILDI_12250 0.69 -0.2
2,458,977 + pdeF BNILDI_12250 0.70 -0.5
2,459,099 + pdeF BNILDI_12250 0.75 +0.6
2,459,099 + pdeF BNILDI_12250 0.75 +0.0
2,459,099 + pdeF BNILDI_12250 0.75 +0.6
2,459,100 - pdeF BNILDI_12250 0.75 -0.6
2,459,100 - pdeF BNILDI_12250 0.75 -1.2
2,459,100 - pdeF BNILDI_12250 0.75 +0.1
2,459,100 - pdeF BNILDI_12250 0.75 -0.7
2,459,100 - pdeF BNILDI_12250 0.75 -1.0
2,459,153 + pdeF BNILDI_12250 0.78 -0.3
2,459,153 + pdeF BNILDI_12250 0.78 +2.5
2,459,157 - pdeF BNILDI_12250 0.78 -2.2
2,459,220 - pdeF BNILDI_12250 0.81 -0.3
2,459,246 + pdeF BNILDI_12250 0.82 -0.7
2,459,433 - +0.8
2,459,462 - -0.1
2,459,517 + +0.7
2,459,518 - +0.6
2,459,518 - +0.2
2,459,569 - +1.9
2,459,570 + +0.4
2,459,570 + -1.0
2,459,591 + -0.5
2,459,758 - +0.3
2,459,804 - -0.2
2,459,900 + ppx BNILDI_12255 0.14 +0.3
2,459,901 - ppx BNILDI_12255 0.14 +0.5
2,459,903 + ppx BNILDI_12255 0.14 -0.1
2,459,918 + ppx BNILDI_12255 0.15 -0.3
2,459,925 - ppx BNILDI_12255 0.15 +1.4
2,459,951 - ppx BNILDI_12255 0.17 -0.2
2,459,959 + ppx BNILDI_12255 0.17 +0.1
2,459,959 + ppx BNILDI_12255 0.17 -1.1
2,460,072 + ppx BNILDI_12255 0.25 +0.7
2,460,202 - ppx BNILDI_12255 0.33 +0.9
2,460,325 - ppx BNILDI_12255 0.41 +1.9
2,460,342 - ppx BNILDI_12255 0.42 -0.3
2,460,377 + ppx BNILDI_12255 0.45 -0.9

Or see this region's nucleotide sequence