Experiment: MPec2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Arg_trna and yqaB are separated by 280 nucleotides yqaB and ygaA overlap by 4 nucleotides ygaA and gshA are separated by 72 nucleotides
BNILDI_11145: Arg_trna - tRNA-Arg(acg), at 2,249,840 to 2,249,916
_trna
BNILDI_11150: yqaB - fructose-1-phosphate/6-phosphogluconate phosphatase, at 2,250,197 to 2,250,763
yqaB
BNILDI_11155: ygaA - SNARE associated Golgi protein, at 2,250,760 to 2,251,188
ygaA
BNILDI_11160: gshA - glutamate--cysteine ligase, at 2,251,261 to 2,252,817
gshA
Position (kb)
2250
2251
2252 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2249.917 kb on + strand at 2249.917 kb on + strand at 2249.962 kb on + strand at 2249.962 kb on + strand at 2249.965 kb on + strand at 2250.156 kb on - strand at 2250.165 kb on + strand at 2250.200 kb on + strand at 2250.201 kb on - strand at 2250.220 kb on - strand at 2250.286 kb on - strand, within yqaB at 2250.355 kb on + strand, within yqaB at 2250.439 kb on + strand, within yqaB at 2250.439 kb on + strand, within yqaB at 2250.659 kb on - strand, within yqaB at 2250.813 kb on + strand, within ygaA at 2250.875 kb on - strand, within ygaA at 2250.912 kb on + strand, within ygaA at 2250.913 kb on - strand, within ygaA at 2250.913 kb on - strand, within ygaA at 2250.931 kb on - strand, within ygaA at 2250.931 kb on - strand, within ygaA at 2250.964 kb on - strand, within ygaA at 2251.026 kb on - strand, within ygaA at 2251.029 kb on - strand, within ygaA at 2251.031 kb on + strand, within ygaA at 2251.058 kb on + strand, within ygaA at 2251.059 kb on - strand, within ygaA at 2251.186 kb on + strand at 2251.187 kb on - strand at 2251.192 kb on + strand at 2251.204 kb on + strand at 2251.205 kb on - strand at 2251.205 kb on - strand at 2251.205 kb on - strand at 2251.222 kb on + strand at 2251.231 kb on + strand at 2251.274 kb on + strand at 2251.275 kb on - strand at 2251.359 kb on + strand at 2251.404 kb on - strand at 2251.468 kb on - strand, within gshA at 2251.494 kb on + strand, within gshA at 2251.494 kb on + strand, within gshA at 2251.494 kb on + strand, within gshA at 2251.495 kb on - strand, within gshA at 2251.507 kb on - strand, within gshA at 2251.527 kb on + strand, within gshA at 2251.570 kb on - strand, within gshA at 2251.579 kb on + strand, within gshA at 2251.626 kb on + strand, within gshA at 2251.627 kb on - strand, within gshA at 2251.758 kb on + strand, within gshA at 2251.819 kb on + strand, within gshA at 2251.820 kb on - strand, within gshA at 2251.932 kb on + strand, within gshA at 2251.933 kb on - strand, within gshA at 2251.936 kb on + strand, within gshA at 2251.937 kb on - strand, within gshA at 2251.937 kb on - strand, within gshA at 2251.937 kb on - strand, within gshA at 2251.940 kb on - strand, within gshA at 2251.940 kb on - strand, within gshA at 2251.984 kb on - strand, within gshA at 2252.094 kb on + strand, within gshA
Per-strain Table
Position Strand Gene LocusTag Fraction MPec2 remove 2,249,917 + -0.6 2,249,917 + +0.7 2,249,962 + -0.3 2,249,962 + -0.7 2,249,965 + +0.5 2,250,156 - -0.6 2,250,165 + +0.2 2,250,200 + +0.2 2,250,201 - -0.0 2,250,220 - -0.4 2,250,286 - yqaB BNILDI_11150 0.16 -0.6 2,250,355 + yqaB BNILDI_11150 0.28 -0.2 2,250,439 + yqaB BNILDI_11150 0.43 +4.0 2,250,439 + yqaB BNILDI_11150 0.43 -2.0 2,250,659 - yqaB BNILDI_11150 0.81 +1.1 2,250,813 + ygaA BNILDI_11155 0.12 +0.7 2,250,875 - ygaA BNILDI_11155 0.27 -0.6 2,250,912 + ygaA BNILDI_11155 0.35 +1.0 2,250,913 - ygaA BNILDI_11155 0.36 +1.0 2,250,913 - ygaA BNILDI_11155 0.36 -0.4 2,250,931 - ygaA BNILDI_11155 0.40 -0.5 2,250,931 - ygaA BNILDI_11155 0.40 -0.7 2,250,964 - ygaA BNILDI_11155 0.48 -0.0 2,251,026 - ygaA BNILDI_11155 0.62 +0.8 2,251,029 - ygaA BNILDI_11155 0.63 +1.0 2,251,031 + ygaA BNILDI_11155 0.63 +1.4 2,251,058 + ygaA BNILDI_11155 0.69 -0.4 2,251,059 - ygaA BNILDI_11155 0.70 +1.4 2,251,186 + -0.2 2,251,187 - -0.2 2,251,192 + -0.7 2,251,204 + +1.4 2,251,205 - -1.0 2,251,205 - +0.7 2,251,205 - +0.1 2,251,222 + -0.4 2,251,231 + +0.4 2,251,274 + -0.5 2,251,275 - -0.0 2,251,359 + +0.4 2,251,404 - +1.0 2,251,468 - gshA BNILDI_11160 0.13 -1.9 2,251,494 + gshA BNILDI_11160 0.15 -1.4 2,251,494 + gshA BNILDI_11160 0.15 -1.0 2,251,494 + gshA BNILDI_11160 0.15 +2.0 2,251,495 - gshA BNILDI_11160 0.15 +0.4 2,251,507 - gshA BNILDI_11160 0.16 -0.8 2,251,527 + gshA BNILDI_11160 0.17 -0.0 2,251,570 - gshA BNILDI_11160 0.20 +0.5 2,251,579 + gshA BNILDI_11160 0.20 +1.2 2,251,626 + gshA BNILDI_11160 0.23 -2.6 2,251,627 - gshA BNILDI_11160 0.24 -0.0 2,251,758 + gshA BNILDI_11160 0.32 -0.8 2,251,819 + gshA BNILDI_11160 0.36 +0.7 2,251,820 - gshA BNILDI_11160 0.36 -1.4 2,251,932 + gshA BNILDI_11160 0.43 -0.8 2,251,933 - gshA BNILDI_11160 0.43 +1.0 2,251,936 + gshA BNILDI_11160 0.43 +1.0 2,251,937 - gshA BNILDI_11160 0.43 -0.9 2,251,937 - gshA BNILDI_11160 0.43 +0.6 2,251,937 - gshA BNILDI_11160 0.43 +2.0 2,251,940 - gshA BNILDI_11160 0.44 +1.4 2,251,940 - gshA BNILDI_11160 0.44 +0.4 2,251,984 - gshA BNILDI_11160 0.46 -0.5 2,252,094 + gshA BNILDI_11160 0.54 -2.8
Or see this region's nucleotide sequence