Strain Fitness in Escherichia coli ECRC62 around BNILDI_10815

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysN and cysC overlap by 1 nucleotidescysC and ygbE are separated by 49 nucleotidesygbE and ftsB are separated by 193 nucleotidesftsB and ispD are separated by 18 nucleotides BNILDI_10810: cysN - sulfate adenylyltransferase subunit CysN, at 2,187,841 to 2,189,268 cysN BNILDI_10815: cysC - Adenylyl-sulfate kinase, at 2,189,268 to 2,189,873 cysC BNILDI_10820: ygbE - DUF3561 domain-containing protein, at 2,189,923 to 2,190,246 ygbE BNILDI_10825: ftsB - cell division protein FtsB, at 2,190,440 to 2,190,751 ftsB BNILDI_10830: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 2,190,770 to 2,191,480 ispD Position (kb) 2189 2190Strain fitness (log2 ratio) -2 -1 0 1 2at 2188.335 kb on - strand, within cysNat 2188.421 kb on + strand, within cysNat 2188.422 kb on - strand, within cysNat 2188.497 kb on + strand, within cysNat 2188.497 kb on + strand, within cysNat 2188.498 kb on - strand, within cysNat 2188.595 kb on - strand, within cysNat 2188.595 kb on - strand, within cysNat 2188.595 kb on - strand, within cysNat 2188.903 kb on + strand, within cysNat 2188.903 kb on + strand, within cysNat 2189.058 kb on - strand, within cysNat 2189.058 kb on - strand, within cysNat 2189.263 kb on - strandat 2189.267 kb on - strandat 2189.267 kb on - strandat 2189.333 kb on - strand, within cysCat 2189.333 kb on - strand, within cysCat 2189.353 kb on + strand, within cysCat 2189.353 kb on + strand, within cysCat 2189.388 kb on - strand, within cysCat 2189.422 kb on + strand, within cysCat 2189.427 kb on - strand, within cysCat 2189.441 kb on + strand, within cysCat 2189.441 kb on + strand, within cysCat 2189.442 kb on - strand, within cysCat 2189.445 kb on + strand, within cysCat 2189.445 kb on + strand, within cysCat 2189.446 kb on - strand, within cysCat 2189.446 kb on - strand, within cysCat 2189.446 kb on - strand, within cysCat 2189.513 kb on + strand, within cysCat 2189.588 kb on + strand, within cysCat 2189.588 kb on + strand, within cysCat 2189.649 kb on - strand, within cysCat 2189.708 kb on - strand, within cysCat 2189.708 kb on - strand, within cysCat 2189.710 kb on - strand, within cysCat 2189.711 kb on + strand, within cysCat 2189.711 kb on + strand, within cysCat 2189.711 kb on + strand, within cysCat 2189.712 kb on - strand, within cysCat 2189.834 kb on - strandat 2189.834 kb on - strandat 2189.861 kb on + strandat 2189.861 kb on + strandat 2190.018 kb on + strand, within ygbEat 2190.018 kb on + strand, within ygbEat 2190.018 kb on + strand, within ygbEat 2190.058 kb on + strand, within ygbEat 2190.059 kb on - strand, within ygbEat 2190.084 kb on + strand, within ygbEat 2190.084 kb on + strand, within ygbEat 2190.085 kb on - strand, within ygbEat 2190.088 kb on + strand, within ygbEat 2190.088 kb on + strand, within ygbEat 2190.171 kb on - strand, within ygbEat 2190.171 kb on - strand, within ygbEat 2190.175 kb on + strand, within ygbEat 2190.176 kb on - strand, within ygbEat 2190.203 kb on + strand, within ygbEat 2190.210 kb on + strand, within ygbEat 2190.228 kb on + strandat 2190.229 kb on - strandat 2190.244 kb on + strandat 2190.251 kb on + strandat 2190.274 kb on + strandat 2190.290 kb on + strandat 2190.291 kb on - strandat 2190.293 kb on + strandat 2190.302 kb on + strandat 2190.303 kb on - strandat 2190.303 kb on - strandat 2190.323 kb on - strandat 2190.324 kb on + strandat 2190.325 kb on - strandat 2190.325 kb on - strandat 2190.325 kb on - strandat 2190.325 kb on - strandat 2190.328 kb on + strandat 2190.329 kb on - strandat 2190.329 kb on - strandat 2190.329 kb on - strandat 2190.329 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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2,188,335 - cysN BNILDI_10810 0.35 -0.3
2,188,421 + cysN BNILDI_10810 0.41 -0.6
2,188,422 - cysN BNILDI_10810 0.41 +0.8
2,188,497 + cysN BNILDI_10810 0.46 +0.0
2,188,497 + cysN BNILDI_10810 0.46 +0.2
2,188,498 - cysN BNILDI_10810 0.46 -0.2
2,188,595 - cysN BNILDI_10810 0.53 +2.6
2,188,595 - cysN BNILDI_10810 0.53 -1.3
2,188,595 - cysN BNILDI_10810 0.53 -0.4
2,188,903 + cysN BNILDI_10810 0.74 +0.1
2,188,903 + cysN BNILDI_10810 0.74 +0.4
2,189,058 - cysN BNILDI_10810 0.85 +1.2
2,189,058 - cysN BNILDI_10810 0.85 +2.0
2,189,263 - -1.9
2,189,267 - -0.7
2,189,267 - +1.1
2,189,333 - cysC BNILDI_10815 0.11 +0.5
2,189,333 - cysC BNILDI_10815 0.11 +1.4
2,189,353 + cysC BNILDI_10815 0.14 -1.2
2,189,353 + cysC BNILDI_10815 0.14 -1.2
2,189,388 - cysC BNILDI_10815 0.20 -0.4
2,189,422 + cysC BNILDI_10815 0.25 +2.0
2,189,427 - cysC BNILDI_10815 0.26 -0.6
2,189,441 + cysC BNILDI_10815 0.29 -0.4
2,189,441 + cysC BNILDI_10815 0.29 +1.0
2,189,442 - cysC BNILDI_10815 0.29 -0.2
2,189,445 + cysC BNILDI_10815 0.29 +0.7
2,189,445 + cysC BNILDI_10815 0.29 +0.3
2,189,446 - cysC BNILDI_10815 0.29 +1.2
2,189,446 - cysC BNILDI_10815 0.29 -0.1
2,189,446 - cysC BNILDI_10815 0.29 +0.7
2,189,513 + cysC BNILDI_10815 0.40 +2.0
2,189,588 + cysC BNILDI_10815 0.53 -0.7
2,189,588 + cysC BNILDI_10815 0.53 -0.6
2,189,649 - cysC BNILDI_10815 0.63 -1.5
2,189,708 - cysC BNILDI_10815 0.73 -1.0
2,189,708 - cysC BNILDI_10815 0.73 -0.4
2,189,710 - cysC BNILDI_10815 0.73 +0.1
2,189,711 + cysC BNILDI_10815 0.73 -0.1
2,189,711 + cysC BNILDI_10815 0.73 +2.0
2,189,711 + cysC BNILDI_10815 0.73 +0.7
2,189,712 - cysC BNILDI_10815 0.73 +2.0
2,189,834 - +0.2
2,189,834 - -0.6
2,189,861 + +0.3
2,189,861 + +1.0
2,190,018 + ygbE BNILDI_10820 0.29 -1.0
2,190,018 + ygbE BNILDI_10820 0.29 +0.3
2,190,018 + ygbE BNILDI_10820 0.29 -0.9
2,190,058 + ygbE BNILDI_10820 0.42 -1.8
2,190,059 - ygbE BNILDI_10820 0.42 +1.2
2,190,084 + ygbE BNILDI_10820 0.50 +1.0
2,190,084 + ygbE BNILDI_10820 0.50 -0.2
2,190,085 - ygbE BNILDI_10820 0.50 -0.9
2,190,088 + ygbE BNILDI_10820 0.51 -1.9
2,190,088 + ygbE BNILDI_10820 0.51 -1.0
2,190,171 - ygbE BNILDI_10820 0.77 -1.3
2,190,171 - ygbE BNILDI_10820 0.77 +0.1
2,190,175 + ygbE BNILDI_10820 0.78 -0.5
2,190,176 - ygbE BNILDI_10820 0.78 -1.0
2,190,203 + ygbE BNILDI_10820 0.86 +1.1
2,190,210 + ygbE BNILDI_10820 0.89 +0.2
2,190,228 + +1.0
2,190,229 - -0.3
2,190,244 + -0.1
2,190,251 + -1.1
2,190,274 + -0.6
2,190,290 + -0.8
2,190,291 - -0.0
2,190,293 + -2.4
2,190,302 + -0.3
2,190,303 - +1.4
2,190,303 - -0.7
2,190,323 - +0.4
2,190,324 + -1.0
2,190,325 - -0.4
2,190,325 - -1.3
2,190,325 - +0.7
2,190,325 - -0.5
2,190,328 + +0.3
2,190,329 - +1.5
2,190,329 - +0.2
2,190,329 - -0.0
2,190,329 - -0.9

Or see this region's nucleotide sequence