Strain Fitness in Escherichia coli ECRC62 around BNILDI_01645

Experiment: MPec2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrimP and fMet_trna are separated by 206 nucleotidesfMet_trna and argG are separated by 347 nucleotidesargG and yhbX are separated by 7 nucleotides BNILDI_01635: rimP - ribosome maturation factor RimP, at 322,075 to 322,527 rimP BNILDI_01640: fMet_trna - tRNA-fMet(cat), at 322,734 to 322,810 _trna BNILDI_01645: argG - argininosuccinate synthase, at 323,158 to 324,501 argG BNILDI_01650: yhbX - Putative transferase YhbX, at 324,509 to 326,134 yhbX Position (kb) 323 324 325Strain fitness (log2 ratio) -2 -1 0 1 2 3at 322.628 kb on - strandat 322.706 kb on - strandat 322.708 kb on - strandat 322.905 kb on + strandat 322.906 kb on - strandat 322.906 kb on - strandat 323.026 kb on - strandat 323.039 kb on + strandat 323.136 kb on + strandat 323.137 kb on - strandat 323.140 kb on + strandat 323.141 kb on - strandat 323.158 kb on - strandat 323.196 kb on + strandat 323.197 kb on - strandat 323.279 kb on - strandat 323.279 kb on - strandat 323.279 kb on - strandat 323.279 kb on - strandat 323.280 kb on + strandat 323.280 kb on + strandat 323.315 kb on - strand, within argGat 323.315 kb on - strand, within argGat 323.344 kb on + strand, within argGat 323.453 kb on - strand, within argGat 323.552 kb on - strand, within argGat 323.552 kb on - strand, within argGat 323.552 kb on - strand, within argGat 323.552 kb on - strand, within argGat 323.553 kb on + strand, within argGat 323.553 kb on + strand, within argGat 323.553 kb on + strand, within argGat 323.579 kb on - strand, within argGat 323.579 kb on - strand, within argGat 323.643 kb on + strand, within argGat 323.644 kb on - strand, within argGat 323.683 kb on - strand, within argGat 323.874 kb on - strand, within argGat 324.027 kb on + strand, within argGat 324.056 kb on + strand, within argGat 324.056 kb on + strand, within argGat 324.056 kb on + strand, within argGat 324.056 kb on + strand, within argGat 324.056 kb on + strand, within argGat 324.057 kb on - strand, within argGat 324.057 kb on - strand, within argGat 324.057 kb on - strand, within argGat 324.057 kb on - strand, within argGat 324.057 kb on - strand, within argGat 324.279 kb on + strand, within argGat 324.279 kb on + strand, within argGat 324.302 kb on - strand, within argGat 324.361 kb on - strand, within argGat 324.381 kb on + strandat 324.622 kb on + strandat 324.624 kb on + strandat 324.624 kb on + strandat 324.625 kb on - strandat 324.625 kb on - strandat 324.691 kb on + strand, within yhbXat 324.696 kb on + strand, within yhbXat 324.696 kb on + strand, within yhbXat 324.697 kb on - strand, within yhbXat 324.700 kb on + strand, within yhbXat 324.700 kb on + strand, within yhbXat 324.705 kb on + strand, within yhbXat 324.706 kb on - strand, within yhbXat 324.775 kb on + strand, within yhbXat 324.775 kb on + strand, within yhbXat 324.792 kb on + strand, within yhbXat 324.793 kb on - strand, within yhbXat 324.793 kb on - strand, within yhbXat 324.829 kb on - strand, within yhbXat 324.876 kb on + strand, within yhbXat 324.877 kb on - strand, within yhbXat 324.931 kb on + strand, within yhbXat 324.931 kb on + strand, within yhbXat 324.957 kb on + strand, within yhbXat 325.016 kb on - strand, within yhbXat 325.041 kb on - strand, within yhbXat 325.041 kb on - strand, within yhbXat 325.072 kb on + strand, within yhbXat 325.073 kb on - strand, within yhbXat 325.094 kb on + strand, within yhbXat 325.131 kb on + strand, within yhbXat 325.166 kb on - strand, within yhbXat 325.175 kb on - strand, within yhbXat 325.235 kb on - strand, within yhbXat 325.244 kb on - strand, within yhbXat 325.260 kb on + strand, within yhbXat 325.264 kb on + strand, within yhbXat 325.326 kb on + strand, within yhbXat 325.351 kb on + strand, within yhbXat 325.351 kb on + strand, within yhbXat 325.351 kb on + strand, within yhbXat 325.406 kb on - strand, within yhbXat 325.407 kb on + strand, within yhbXat 325.413 kb on + strand, within yhbXat 325.436 kb on + strand, within yhbXat 325.462 kb on - strand, within yhbX

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Per-strain Table

Position Strand Gene LocusTag Fraction MPec2
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322,628 - -1.6
322,706 - -0.8
322,708 - -0.7
322,905 + +0.5
322,906 - -0.1
322,906 - +2.1
323,026 - +0.0
323,039 + -0.3
323,136 + +0.4
323,137 - -0.5
323,140 + -0.3
323,141 - -0.4
323,158 - -0.0
323,196 + -0.0
323,197 - -0.4
323,279 - -1.0
323,279 - +0.4
323,279 - +1.1
323,279 - +0.8
323,280 + -0.6
323,280 + +0.1
323,315 - argG BNILDI_01645 0.12 -0.1
323,315 - argG BNILDI_01645 0.12 +0.3
323,344 + argG BNILDI_01645 0.14 -1.4
323,453 - argG BNILDI_01645 0.22 +0.9
323,552 - argG BNILDI_01645 0.29 +0.3
323,552 - argG BNILDI_01645 0.29 -0.3
323,552 - argG BNILDI_01645 0.29 +1.5
323,552 - argG BNILDI_01645 0.29 +0.2
323,553 + argG BNILDI_01645 0.29 -0.3
323,553 + argG BNILDI_01645 0.29 -0.2
323,553 + argG BNILDI_01645 0.29 +0.5
323,579 - argG BNILDI_01645 0.31 -0.2
323,579 - argG BNILDI_01645 0.31 -0.9
323,643 + argG BNILDI_01645 0.36 -1.1
323,644 - argG BNILDI_01645 0.36 +0.1
323,683 - argG BNILDI_01645 0.39 -0.7
323,874 - argG BNILDI_01645 0.53 -1.4
324,027 + argG BNILDI_01645 0.65 -0.3
324,056 + argG BNILDI_01645 0.67 +0.5
324,056 + argG BNILDI_01645 0.67 -0.2
324,056 + argG BNILDI_01645 0.67 +0.4
324,056 + argG BNILDI_01645 0.67 +0.0
324,056 + argG BNILDI_01645 0.67 -0.3
324,057 - argG BNILDI_01645 0.67 +0.6
324,057 - argG BNILDI_01645 0.67 -0.5
324,057 - argG BNILDI_01645 0.67 +0.7
324,057 - argG BNILDI_01645 0.67 -0.4
324,057 - argG BNILDI_01645 0.67 -0.0
324,279 + argG BNILDI_01645 0.83 -0.8
324,279 + argG BNILDI_01645 0.83 +0.9
324,302 - argG BNILDI_01645 0.85 +0.2
324,361 - argG BNILDI_01645 0.90 -0.7
324,381 + -0.1
324,622 + -0.0
324,624 + +0.1
324,624 + -0.1
324,625 - -2.2
324,625 - -0.7
324,691 + yhbX BNILDI_01650 0.11 -1.1
324,696 + yhbX BNILDI_01650 0.12 +0.2
324,696 + yhbX BNILDI_01650 0.12 +0.1
324,697 - yhbX BNILDI_01650 0.12 -0.2
324,700 + yhbX BNILDI_01650 0.12 +0.4
324,700 + yhbX BNILDI_01650 0.12 -0.1
324,705 + yhbX BNILDI_01650 0.12 -1.2
324,706 - yhbX BNILDI_01650 0.12 -1.2
324,775 + yhbX BNILDI_01650 0.16 +0.5
324,775 + yhbX BNILDI_01650 0.16 +1.3
324,792 + yhbX BNILDI_01650 0.17 +1.1
324,793 - yhbX BNILDI_01650 0.17 -0.4
324,793 - yhbX BNILDI_01650 0.17 -1.8
324,829 - yhbX BNILDI_01650 0.20 +0.1
324,876 + yhbX BNILDI_01650 0.23 +1.1
324,877 - yhbX BNILDI_01650 0.23 +2.1
324,931 + yhbX BNILDI_01650 0.26 +0.4
324,931 + yhbX BNILDI_01650 0.26 +1.1
324,957 + yhbX BNILDI_01650 0.28 +0.5
325,016 - yhbX BNILDI_01650 0.31 +0.5
325,041 - yhbX BNILDI_01650 0.33 -0.4
325,041 - yhbX BNILDI_01650 0.33 -0.2
325,072 + yhbX BNILDI_01650 0.35 -0.6
325,073 - yhbX BNILDI_01650 0.35 +0.3
325,094 + yhbX BNILDI_01650 0.36 +3.1
325,131 + yhbX BNILDI_01650 0.38 -0.4
325,166 - yhbX BNILDI_01650 0.40 -0.9
325,175 - yhbX BNILDI_01650 0.41 +1.1
325,235 - yhbX BNILDI_01650 0.45 -1.4
325,244 - yhbX BNILDI_01650 0.45 -1.4
325,260 + yhbX BNILDI_01650 0.46 +0.9
325,264 + yhbX BNILDI_01650 0.46 +1.1
325,326 + yhbX BNILDI_01650 0.50 +0.3
325,351 + yhbX BNILDI_01650 0.52 +0.3
325,351 + yhbX BNILDI_01650 0.52 +0.2
325,351 + yhbX BNILDI_01650 0.52 +1.8
325,406 - yhbX BNILDI_01650 0.55 -0.3
325,407 + yhbX BNILDI_01650 0.55 +1.1
325,413 + yhbX BNILDI_01650 0.56 +0.7
325,436 + yhbX BNILDI_01650 0.57 +3.7
325,462 - yhbX BNILDI_01650 0.59 +0.3

Or see this region's nucleotide sequence