Experiment: Bas68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppiD and hupB are separated by 191 nucleotides hupB and lon_P403L are separated by 208 nucleotides lon_P403L and clpX are separated by 187 nucleotides
BNILDI_22660: ppiD - peptidylprolyl isomerase, at 4,558,920 to 4,560,791
ppiD
BNILDI_22665: hupB - DNA-binding protein HU-beta, at 4,560,983 to 4,561,255
hupB
BNILDI_22670: lon_P403L - Lon protease, at 4,561,464 to 4,563,818
_P403L
BNILDI_22680: clpX - ATP-dependent protease ATP-binding subunit ClpX, at 4,564,006 to 4,565,280
clpX
Position (kb) 4561
4562
4563
4564 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4560.544 kb on + strand, within ppiD at 4560.545 kb on - strand, within ppiD at 4560.670 kb on + strand at 4560.670 kb on + strand at 4560.675 kb on - strand at 4560.778 kb on - strand at 4560.796 kb on + strand at 4560.797 kb on - strand at 4560.797 kb on - strand at 4560.838 kb on + strand at 4560.859 kb on - strand at 4560.939 kb on - strand at 4561.122 kb on - strand, within hupB at 4561.130 kb on + strand, within hupB at 4561.131 kb on - strand, within hupB at 4561.131 kb on - strand, within hupB at 4561.175 kb on - strand, within hupB at 4561.182 kb on - strand, within hupB at 4561.271 kb on + strand at 4561.274 kb on - strand at 4561.278 kb on + strand at 4561.278 kb on + strand at 4561.279 kb on - strand at 4561.279 kb on - strand at 4561.320 kb on + strand at 4561.321 kb on - strand at 4561.321 kb on - strand at 4561.323 kb on - strand at 4561.342 kb on + strand at 4561.343 kb on - strand at 4561.355 kb on - strand at 4561.355 kb on - strand at 4561.381 kb on + strand at 4561.382 kb on - strand at 4561.942 kb on + strand, within lon_P403L at 4562.279 kb on + strand, within lon_P403L at 4562.616 kb on - strand, within lon_P403L at 4562.735 kb on + strand, within lon_P403L at 4562.736 kb on - strand, within lon_P403L at 4562.809 kb on + strand, within lon_P403L at 4563.136 kb on + strand, within lon_P403L at 4563.302 kb on + strand, within lon_P403L at 4563.883 kb on + strand at 4563.894 kb on - strand at 4563.894 kb on - strand at 4563.894 kb on - strand at 4563.902 kb on - strand at 4563.903 kb on + strand at 4563.985 kb on + strand at 4563.985 kb on + strand at 4564.044 kb on - strand at 4564.076 kb on - strand at 4564.128 kb on - strand at 4564.128 kb on - strand at 4564.154 kb on - strand, within clpX at 4564.770 kb on - strand, within clpX at 4564.770 kb on - strand, within clpX
Per-strain Table
Position Strand Gene LocusTag Fraction Bas68 remove 4,560,544 + ppiD BNILDI_22660 0.87 -0.3 4,560,545 - ppiD BNILDI_22660 0.87 +0.9 4,560,670 + -0.3 4,560,670 + +1.5 4,560,675 - +0.1 4,560,778 - +0.5 4,560,796 + -0.2 4,560,797 - +0.1 4,560,797 - -1.8 4,560,838 + -0.4 4,560,859 - +1.9 4,560,939 - +1.0 4,561,122 - hupB BNILDI_22665 0.51 +1.9 4,561,130 + hupB BNILDI_22665 0.54 -2.0 4,561,131 - hupB BNILDI_22665 0.54 -0.9 4,561,131 - hupB BNILDI_22665 0.54 -1.1 4,561,175 - hupB BNILDI_22665 0.70 -0.9 4,561,182 - hupB BNILDI_22665 0.73 -1.7 4,561,271 + +0.0 4,561,274 - -0.8 4,561,278 + +0.6 4,561,278 + +0.9 4,561,279 - -0.9 4,561,279 - -0.2 4,561,320 + -0.1 4,561,321 - -0.7 4,561,321 - -0.2 4,561,323 - -0.1 4,561,342 + -0.4 4,561,343 - -0.0 4,561,355 - -0.3 4,561,355 - -0.1 4,561,381 + -1.2 4,561,382 - -0.1 4,561,942 + lon_P403L BNILDI_22670 0.20 +0.5 4,562,279 + lon_P403L BNILDI_22670 0.35 +0.9 4,562,616 - lon_P403L BNILDI_22670 0.49 -0.4 4,562,735 + lon_P403L BNILDI_22670 0.54 +0.3 4,562,736 - lon_P403L BNILDI_22670 0.54 -0.7 4,562,809 + lon_P403L BNILDI_22670 0.57 -0.6 4,563,136 + lon_P403L BNILDI_22670 0.71 -3.5 4,563,302 + lon_P403L BNILDI_22670 0.78 -0.0 4,563,883 + -2.2 4,563,894 - +0.2 4,563,894 - -0.0 4,563,894 - -0.8 4,563,902 - +1.4 4,563,903 + -0.1 4,563,985 + +0.3 4,563,985 + -0.4 4,564,044 - -0.2 4,564,076 - +0.3 4,564,128 - +0.9 4,564,128 - -0.7 4,564,154 - clpX BNILDI_22680 0.12 +0.1 4,564,770 - clpX BNILDI_22680 0.60 +0.9 4,564,770 - clpX BNILDI_22680 0.60 -0.9
Or see this region's nucleotide sequence