Strain Fitness in Escherichia coli ECRC62 around BNILDI_16220

Experiment: Bas68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntastD and astB overlap by 4 nucleotidesastB and astE overlap by 8 nucleotides BNILDI_16215: astD - succinylglutamate-semialdehyde dehydrogenase, at 3,252,283 to 3,253,761 astD BNILDI_16220: astB - N-succinylarginine dihydrolase, at 3,253,758 to 3,255,101 astB BNILDI_16225: astE - succinylglutamate desuccinylase, at 3,255,094 to 3,256,062 astE Position (kb) 3253 3254 3255 3256Strain fitness (log2 ratio) -1 0 1at 3253.130 kb on + strand, within astDat 3253.335 kb on - strand, within astDat 3253.335 kb on - strand, within astDat 3253.473 kb on + strand, within astDat 3253.473 kb on + strand, within astDat 3253.810 kb on - strandat 3253.865 kb on + strandat 3253.894 kb on + strand, within astBat 3253.964 kb on + strand, within astBat 3254.160 kb on - strand, within astBat 3254.664 kb on + strand, within astBat 3254.664 kb on + strand, within astBat 3254.930 kb on + strand, within astBat 3254.958 kb on + strand, within astBat 3255.032 kb on + strandat 3255.428 kb on + strand, within astEat 3255.428 kb on + strand, within astEat 3255.572 kb on + strand, within astEat 3255.572 kb on + strand, within astEat 3255.715 kb on - strand, within astEat 3255.773 kb on + strand, within astEat 3255.918 kb on + strand, within astEat 3255.987 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas68
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3,253,130 + astD BNILDI_16215 0.57 -0.8
3,253,335 - astD BNILDI_16215 0.71 -1.5
3,253,335 - astD BNILDI_16215 0.71 -0.6
3,253,473 + astD BNILDI_16215 0.80 +0.5
3,253,473 + astD BNILDI_16215 0.80 -0.1
3,253,810 - +0.6
3,253,865 + -1.4
3,253,894 + astB BNILDI_16220 0.10 +0.6
3,253,964 + astB BNILDI_16220 0.15 +0.9
3,254,160 - astB BNILDI_16220 0.30 +1.0
3,254,664 + astB BNILDI_16220 0.67 +0.1
3,254,664 + astB BNILDI_16220 0.67 +0.6
3,254,930 + astB BNILDI_16220 0.87 -0.6
3,254,958 + astB BNILDI_16220 0.89 +1.6
3,255,032 + +0.6
3,255,428 + astE BNILDI_16225 0.34 +0.8
3,255,428 + astE BNILDI_16225 0.34 -0.6
3,255,572 + astE BNILDI_16225 0.49 -0.4
3,255,572 + astE BNILDI_16225 0.49 -0.4
3,255,715 - astE BNILDI_16225 0.64 -1.6
3,255,773 + astE BNILDI_16225 0.70 -0.5
3,255,918 + astE BNILDI_16225 0.85 -0.7
3,255,987 - +1.6

Or see this region's nucleotide sequence