Strain Fitness in Escherichia coli ECRC62 around BNILDI_15260

Experiment: Bas68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpgsA and Gly_trna are separated by 151 nucleotidesGly_trna and Cys_trna are separated by 54 nucleotidesCys_trna and Leu_trna are separated by 12 nucleotidesLeu_trna and yecA are separated by 195 nucleotidesyecA and tyrP are separated by 61 nucleotides BNILDI_15240: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 3,069,824 to 3,070,372 pgsA BNILDI_15245: Gly_trna - tRNA-Gly(gcc), at 3,070,524 to 3,070,599 _trna BNILDI_15250: Cys_trna - tRNA-Cys(gca), at 3,070,654 to 3,070,727 _trna BNILDI_15255: Leu_trna - tRNA-Leu(taa), at 3,070,740 to 3,070,826 _trna BNILDI_15260: yecA - UPF0149 family protein YecA, at 3,071,022 to 3,071,687 yecA BNILDI_15265: tyrP - tyrosine transporter TyrP, at 3,071,749 to 3,072,960 tyrP Position (kb) 3071 3072Strain fitness (log2 ratio) -2 -1 0 1 2at 3070.980 kb on + strandat 3070.981 kb on - strandat 3070.981 kb on - strandat 3070.981 kb on - strandat 3070.981 kb on - strandat 3070.981 kb on - strandat 3070.986 kb on - strandat 3071.021 kb on + strandat 3071.205 kb on + strand, within yecAat 3071.205 kb on + strand, within yecAat 3071.206 kb on - strand, within yecAat 3071.267 kb on + strand, within yecAat 3071.268 kb on - strand, within yecAat 3071.382 kb on + strand, within yecAat 3071.382 kb on + strand, within yecAat 3071.382 kb on + strand, within yecAat 3071.385 kb on - strand, within yecAat 3071.524 kb on + strand, within yecAat 3071.524 kb on + strand, within yecAat 3071.524 kb on + strand, within yecAat 3071.525 kb on - strand, within yecAat 3071.743 kb on - strandat 3072.079 kb on + strand, within tyrPat 3072.131 kb on - strand, within tyrPat 3072.197 kb on - strand, within tyrPat 3072.274 kb on - strand, within tyrPat 3072.320 kb on - strand, within tyrPat 3072.343 kb on - strand, within tyrPat 3072.344 kb on + strand, within tyrPat 3072.344 kb on + strand, within tyrPat 3072.345 kb on - strand, within tyrPat 3072.345 kb on - strand, within tyrPat 3072.345 kb on - strand, within tyrPat 3072.388 kb on - strand, within tyrPat 3072.460 kb on - strand, within tyrPat 3072.616 kb on - strand, within tyrPat 3072.669 kb on - strand, within tyrP

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas68
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3,070,980 + -0.1
3,070,981 - -0.3
3,070,981 - -0.1
3,070,981 - +1.4
3,070,981 - -1.1
3,070,981 - -0.4
3,070,986 - +0.1
3,071,021 + -0.0
3,071,205 + yecA BNILDI_15260 0.27 -1.9
3,071,205 + yecA BNILDI_15260 0.27 +0.3
3,071,206 - yecA BNILDI_15260 0.28 +0.4
3,071,267 + yecA BNILDI_15260 0.37 -0.1
3,071,268 - yecA BNILDI_15260 0.37 +1.1
3,071,382 + yecA BNILDI_15260 0.54 +0.1
3,071,382 + yecA BNILDI_15260 0.54 -0.7
3,071,382 + yecA BNILDI_15260 0.54 +2.4
3,071,385 - yecA BNILDI_15260 0.55 +2.2
3,071,524 + yecA BNILDI_15260 0.75 +0.1
3,071,524 + yecA BNILDI_15260 0.75 +0.9
3,071,524 + yecA BNILDI_15260 0.75 -1.5
3,071,525 - yecA BNILDI_15260 0.76 +1.1
3,071,743 - -0.2
3,072,079 + tyrP BNILDI_15265 0.27 +0.4
3,072,131 - tyrP BNILDI_15265 0.32 +0.6
3,072,197 - tyrP BNILDI_15265 0.37 +0.2
3,072,274 - tyrP BNILDI_15265 0.43 +0.3
3,072,320 - tyrP BNILDI_15265 0.47 -1.0
3,072,343 - tyrP BNILDI_15265 0.49 -0.1
3,072,344 + tyrP BNILDI_15265 0.49 -0.0
3,072,344 + tyrP BNILDI_15265 0.49 +0.9
3,072,345 - tyrP BNILDI_15265 0.49 -0.5
3,072,345 - tyrP BNILDI_15265 0.49 +0.1
3,072,345 - tyrP BNILDI_15265 0.49 +0.1
3,072,388 - tyrP BNILDI_15265 0.53 -0.7
3,072,460 - tyrP BNILDI_15265 0.59 +1.0
3,072,616 - tyrP BNILDI_15265 0.72 +1.3
3,072,669 - tyrP BNILDI_15265 0.76 -0.7

Or see this region's nucleotide sequence