Strain Fitness in Escherichia coli ECRC62 around BNILDI_12635

Experiment: Bas68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteutL and eutK are separated by 12 nucleotideseutK and eutR are separated by 45 nucleotideseutR and hemF are separated by 5 nucleotideshemF and amiA are separated by 3 nucleotides BNILDI_12625: eutL - ethanolamine utilization microcompartment protein EutL, at 2,532,551 to 2,533,210 eutL BNILDI_12630: eutK - ethanolamine utilization microcompartment protein EutK, at 2,533,223 to 2,533,723 eutK BNILDI_12635: eutR - HTH-type transcriptional regulator EutR, at 2,533,769 to 2,534,821 eutR BNILDI_12640: hemF - oxygen-dependent coproporphyrinogen oxidase, at 2,534,827 to 2,535,726 hemF BNILDI_12645: amiA - N-acetylmuramoyl-L-alanine amidase AmiA, at 2,535,730 to 2,536,599 amiA Position (kb) 2533 2534 2535Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2532.953 kb on + strand, within eutLat 2532.954 kb on - strand, within eutLat 2532.954 kb on - strand, within eutLat 2533.028 kb on + strand, within eutLat 2533.076 kb on + strand, within eutLat 2533.304 kb on - strand, within eutKat 2533.584 kb on + strand, within eutKat 2533.585 kb on - strand, within eutKat 2533.585 kb on - strand, within eutKat 2533.585 kb on - strand, within eutKat 2533.585 kb on - strand, within eutKat 2533.585 kb on - strand, within eutKat 2533.696 kb on + strandat 2533.747 kb on + strandat 2533.784 kb on - strandat 2533.805 kb on + strandat 2533.805 kb on + strandat 2533.858 kb on + strandat 2533.890 kb on - strand, within eutRat 2533.890 kb on - strand, within eutRat 2534.007 kb on - strand, within eutRat 2534.256 kb on + strand, within eutRat 2534.257 kb on - strand, within eutRat 2534.257 kb on - strand, within eutRat 2534.260 kb on + strand, within eutRat 2534.260 kb on + strand, within eutRat 2534.260 kb on + strand, within eutRat 2534.261 kb on - strand, within eutRat 2534.269 kb on + strand, within eutRat 2534.480 kb on + strand, within eutRat 2534.490 kb on + strand, within eutRat 2534.490 kb on + strand, within eutRat 2534.491 kb on - strand, within eutRat 2534.510 kb on + strand, within eutRat 2534.510 kb on + strand, within eutRat 2534.511 kb on - strand, within eutRat 2534.587 kb on - strand, within eutRat 2534.701 kb on - strand, within eutRat 2534.847 kb on + strandat 2534.848 kb on - strandat 2534.860 kb on + strandat 2534.900 kb on + strandat 2535.008 kb on + strand, within hemFat 2535.024 kb on - strand, within hemFat 2535.066 kb on + strand, within hemFat 2535.066 kb on + strand, within hemFat 2535.067 kb on - strand, within hemFat 2535.077 kb on - strand, within hemFat 2535.085 kb on + strand, within hemFat 2535.085 kb on + strand, within hemFat 2535.123 kb on + strand, within hemFat 2535.213 kb on - strand, within hemFat 2535.324 kb on - strand, within hemFat 2535.426 kb on - strand, within hemFat 2535.433 kb on - strand, within hemFat 2535.624 kb on + strand, within hemFat 2535.727 kb on - strandat 2535.777 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas68
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2,532,953 + eutL BNILDI_12625 0.61 -0.5
2,532,954 - eutL BNILDI_12625 0.61 -0.0
2,532,954 - eutL BNILDI_12625 0.61 +0.8
2,533,028 + eutL BNILDI_12625 0.72 +0.3
2,533,076 + eutL BNILDI_12625 0.80 -0.1
2,533,304 - eutK BNILDI_12630 0.16 +0.7
2,533,584 + eutK BNILDI_12630 0.72 -0.2
2,533,585 - eutK BNILDI_12630 0.72 +0.1
2,533,585 - eutK BNILDI_12630 0.72 -0.4
2,533,585 - eutK BNILDI_12630 0.72 +0.3
2,533,585 - eutK BNILDI_12630 0.72 +1.5
2,533,585 - eutK BNILDI_12630 0.72 -0.2
2,533,696 + +1.4
2,533,747 + +0.4
2,533,784 - +0.8
2,533,805 + -0.4
2,533,805 + +2.8
2,533,858 + +0.2
2,533,890 - eutR BNILDI_12635 0.11 +0.2
2,533,890 - eutR BNILDI_12635 0.11 -0.5
2,534,007 - eutR BNILDI_12635 0.23 -0.7
2,534,256 + eutR BNILDI_12635 0.46 +0.8
2,534,257 - eutR BNILDI_12635 0.46 +0.4
2,534,257 - eutR BNILDI_12635 0.46 +0.3
2,534,260 + eutR BNILDI_12635 0.47 -0.2
2,534,260 + eutR BNILDI_12635 0.47 -0.3
2,534,260 + eutR BNILDI_12635 0.47 +1.3
2,534,261 - eutR BNILDI_12635 0.47 +0.5
2,534,269 + eutR BNILDI_12635 0.47 +0.2
2,534,480 + eutR BNILDI_12635 0.68 +0.0
2,534,490 + eutR BNILDI_12635 0.68 +0.3
2,534,490 + eutR BNILDI_12635 0.68 -1.3
2,534,491 - eutR BNILDI_12635 0.69 +0.0
2,534,510 + eutR BNILDI_12635 0.70 -1.0
2,534,510 + eutR BNILDI_12635 0.70 +0.0
2,534,511 - eutR BNILDI_12635 0.70 -0.7
2,534,587 - eutR BNILDI_12635 0.78 +0.6
2,534,701 - eutR BNILDI_12635 0.89 +0.3
2,534,847 + +0.1
2,534,848 - +1.3
2,534,860 + -1.0
2,534,900 + +0.7
2,535,008 + hemF BNILDI_12640 0.20 -0.7
2,535,024 - hemF BNILDI_12640 0.22 -1.5
2,535,066 + hemF BNILDI_12640 0.27 -0.1
2,535,066 + hemF BNILDI_12640 0.27 +0.1
2,535,067 - hemF BNILDI_12640 0.27 +0.3
2,535,077 - hemF BNILDI_12640 0.28 +0.1
2,535,085 + hemF BNILDI_12640 0.29 +0.1
2,535,085 + hemF BNILDI_12640 0.29 -0.7
2,535,123 + hemF BNILDI_12640 0.33 +0.3
2,535,213 - hemF BNILDI_12640 0.43 +0.0
2,535,324 - hemF BNILDI_12640 0.55 +0.1
2,535,426 - hemF BNILDI_12640 0.67 -0.2
2,535,433 - hemF BNILDI_12640 0.67 +0.8
2,535,624 + hemF BNILDI_12640 0.89 -1.9
2,535,727 - -0.3
2,535,777 - +0.8

Or see this region's nucleotide sequence