Experiment: Bas68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroB and aroK are separated by 56 nucleotides aroK and hofQ are separated by 400 nucleotides
BNILDI_02615: aroB - 3-dehydroquinate synthase, at 494,136 to 495,224
aroB
BNILDI_02620: aroK - shikimate kinase AroK, at 495,281 to 495,802
aroK
BNILDI_02625: hofQ - DNA uptake porin HofQ, at 496,203 to 497,411
hofQ
Position (kb) 495
496 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 494.282 kb on - strand, within aroB at 494.282 kb on - strand, within aroB at 494.344 kb on - strand, within aroB at 494.345 kb on + strand, within aroB at 494.346 kb on - strand, within aroB at 494.346 kb on - strand, within aroB at 494.346 kb on - strand, within aroB at 494.346 kb on - strand, within aroB at 494.349 kb on - strand, within aroB at 494.370 kb on - strand, within aroB at 494.436 kb on + strand, within aroB at 494.436 kb on + strand, within aroB at 494.437 kb on - strand, within aroB at 494.570 kb on + strand, within aroB at 494.571 kb on - strand, within aroB at 494.662 kb on + strand, within aroB at 494.662 kb on + strand, within aroB at 494.752 kb on + strand, within aroB at 494.833 kb on - strand, within aroB at 494.873 kb on - strand, within aroB at 494.908 kb on - strand, within aroB at 494.986 kb on - strand, within aroB at 495.000 kb on - strand, within aroB at 495.000 kb on - strand, within aroB at 495.001 kb on + strand, within aroB at 495.179 kb on + strand at 495.179 kb on + strand at 495.192 kb on - strand at 495.192 kb on - strand at 495.207 kb on - strand at 495.245 kb on + strand at 495.267 kb on - strand at 495.267 kb on - strand at 495.268 kb on + strand at 495.268 kb on + strand at 495.269 kb on - strand at 495.270 kb on + strand at 495.270 kb on + strand at 495.271 kb on - strand at 495.271 kb on - strand at 495.305 kb on - strand at 495.373 kb on - strand, within aroK at 495.499 kb on - strand, within aroK at 495.644 kb on + strand, within aroK at 495.815 kb on - strand at 495.818 kb on - strand at 495.885 kb on + strand at 495.886 kb on - strand at 495.886 kb on - strand at 495.969 kb on - strand at 495.969 kb on - strand at 496.070 kb on - strand at 496.070 kb on - strand at 496.130 kb on + strand at 496.131 kb on - strand at 496.135 kb on + strand at 496.136 kb on - strand at 496.136 kb on - strand at 496.136 kb on - strand at 496.136 kb on - strand at 496.139 kb on + strand at 496.140 kb on - strand at 496.233 kb on - strand at 496.233 kb on - strand at 496.282 kb on - strand at 496.317 kb on + strand at 496.318 kb on - strand at 496.318 kb on - strand at 496.322 kb on + strand at 496.343 kb on + strand, within hofQ at 496.449 kb on + strand, within hofQ at 496.449 kb on + strand, within hofQ at 496.449 kb on + strand, within hofQ at 496.449 kb on + strand, within hofQ at 496.510 kb on - strand, within hofQ at 496.551 kb on - strand, within hofQ at 496.551 kb on - strand, within hofQ at 496.616 kb on + strand, within hofQ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas68 remove 494,282 - aroB BNILDI_02615 0.13 -0.5 494,282 - aroB BNILDI_02615 0.13 -0.2 494,344 - aroB BNILDI_02615 0.19 +0.0 494,345 + aroB BNILDI_02615 0.19 -1.7 494,346 - aroB BNILDI_02615 0.19 +3.2 494,346 - aroB BNILDI_02615 0.19 -2.3 494,346 - aroB BNILDI_02615 0.19 -0.3 494,346 - aroB BNILDI_02615 0.19 -0.4 494,349 - aroB BNILDI_02615 0.20 -0.5 494,370 - aroB BNILDI_02615 0.21 +0.2 494,436 + aroB BNILDI_02615 0.28 +0.5 494,436 + aroB BNILDI_02615 0.28 +0.5 494,437 - aroB BNILDI_02615 0.28 -0.7 494,570 + aroB BNILDI_02615 0.40 -1.7 494,571 - aroB BNILDI_02615 0.40 -2.0 494,662 + aroB BNILDI_02615 0.48 +0.5 494,662 + aroB BNILDI_02615 0.48 -0.3 494,752 + aroB BNILDI_02615 0.57 -1.7 494,833 - aroB BNILDI_02615 0.64 -0.8 494,873 - aroB BNILDI_02615 0.68 -1.0 494,908 - aroB BNILDI_02615 0.71 +1.4 494,986 - aroB BNILDI_02615 0.78 -0.4 495,000 - aroB BNILDI_02615 0.79 -0.1 495,000 - aroB BNILDI_02615 0.79 -0.1 495,001 + aroB BNILDI_02615 0.79 -0.3 495,179 + -1.0 495,179 + +1.0 495,192 - -0.4 495,192 - +0.2 495,207 - +0.7 495,245 + -0.3 495,267 - +0.3 495,267 - +0.0 495,268 + -1.1 495,268 + -0.5 495,269 - +1.6 495,270 + -0.3 495,270 + -0.9 495,271 - -0.5 495,271 - +0.0 495,305 - -1.0 495,373 - aroK BNILDI_02620 0.18 +2.0 495,499 - aroK BNILDI_02620 0.42 +0.0 495,644 + aroK BNILDI_02620 0.70 +0.5 495,815 - -0.3 495,818 - +2.0 495,885 + +2.0 495,886 - -0.4 495,886 - -0.3 495,969 - -0.5 495,969 - -1.5 496,070 - -0.3 496,070 - -1.7 496,130 + -0.3 496,131 - -0.2 496,135 + +0.5 496,136 - +0.2 496,136 - -1.9 496,136 - -0.1 496,136 - -0.0 496,139 + -1.5 496,140 - +1.3 496,233 - +1.3 496,233 - -1.9 496,282 - +0.5 496,317 + -0.2 496,318 - -0.1 496,318 - -1.1 496,322 + +0.7 496,343 + hofQ BNILDI_02625 0.12 -0.1 496,449 + hofQ BNILDI_02625 0.20 +0.3 496,449 + hofQ BNILDI_02625 0.20 +0.2 496,449 + hofQ BNILDI_02625 0.20 +0.7 496,449 + hofQ BNILDI_02625 0.20 -0.7 496,510 - hofQ BNILDI_02625 0.25 +0.1 496,551 - hofQ BNILDI_02625 0.29 +0.2 496,551 - hofQ BNILDI_02625 0.29 -0.4 496,616 + hofQ BNILDI_02625 0.34 +1.0
Or see this region's nucleotide sequence