Strain Fitness in Escherichia coli ECRC62 around BNILDI_01790

Experiment: Bas68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlptC and lptA overlap by 32 nucleotideslptA and lptB are separated by 6 nucleotideslptB and rpoN are separated by 47 nucleotides BNILDI_01780: lptC - LPS export ABC transporter periplasmic protein LptC, at 347,210 to 347,785 lptC BNILDI_01785: lptA - lipopolysaccharide ABC transporter substrate-binding protein LptA, at 347,754 to 348,311 lptA BNILDI_01790: lptB - LPS export ABC transporter ATP-binding protein, at 348,318 to 349,043 lptB BNILDI_01795: rpoN - RNA polymerase factor sigma-54, at 349,091 to 350,524 rpoN Position (kb) 348 349 350Strain fitness (log2 ratio) -1 0 1at 347.638 kb on + strand, within lptCat 347.638 kb on + strand, within lptCat 347.663 kb on + strand, within lptCat 347.663 kb on + strand, within lptCat 347.663 kb on + strand, within lptC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas68
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347,638 + lptC BNILDI_01780 0.74 +0.4
347,638 + lptC BNILDI_01780 0.74 -1.0
347,663 + lptC BNILDI_01780 0.79 -1.0
347,663 + lptC BNILDI_01780 0.79 -0.4
347,663 + lptC BNILDI_01780 0.79 -1.0

Or see this region's nucleotide sequence