Strain Fitness in Escherichia coli ECRC62 around BNILDI_01010

Experiment: Bas68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygiF and ygiM are separated by 241 nucleotidesygiM and cca are separated by 63 nucleotides BNILDI_01005: ygiF - inorganic triphosphatase, at 193,324 to 194,625 ygiF BNILDI_01010: ygiM - TIGR04211 family SH3 domain-containing protein, at 194,867 to 195,487 ygiM BNILDI_01015: cca - fused tRNA nucleotidyltransferase/2',3'-cyclic phosphodiesterase/2' nucleotidase/phosphatase Cca, at 195,551 to 196,789 cca Position (kb) 194 195 196Strain fitness (log2 ratio) -1 0 1 2 3at 193.913 kb on + strand, within ygiFat 193.913 kb on + strand, within ygiFat 193.914 kb on - strand, within ygiFat 194.160 kb on + strand, within ygiFat 194.278 kb on + strand, within ygiFat 194.332 kb on + strand, within ygiFat 194.374 kb on - strand, within ygiFat 194.376 kb on - strand, within ygiFat 194.392 kb on + strand, within ygiFat 194.436 kb on - strand, within ygiFat 194.457 kb on + strand, within ygiFat 194.461 kb on + strand, within ygiFat 194.512 kb on - strandat 194.592 kb on + strandat 194.598 kb on + strandat 194.632 kb on + strandat 194.633 kb on - strandat 194.633 kb on - strandat 194.633 kb on - strandat 194.636 kb on + strandat 194.644 kb on - strandat 194.644 kb on - strandat 194.652 kb on + strandat 194.652 kb on + strandat 194.653 kb on - strandat 194.653 kb on - strandat 194.675 kb on + strandat 194.697 kb on - strandat 194.700 kb on + strandat 194.701 kb on - strandat 194.703 kb on + strandat 194.704 kb on - strandat 194.828 kb on + strandat 194.829 kb on - strandat 194.832 kb on - strandat 194.892 kb on + strandat 194.965 kb on + strand, within ygiMat 195.039 kb on + strand, within ygiMat 195.042 kb on + strand, within ygiMat 195.043 kb on - strand, within ygiMat 195.065 kb on + strand, within ygiMat 195.066 kb on - strand, within ygiMat 195.066 kb on - strand, within ygiMat 195.066 kb on - strand, within ygiMat 195.122 kb on - strand, within ygiMat 195.345 kb on + strand, within ygiMat 195.345 kb on + strand, within ygiMat 195.388 kb on + strand, within ygiMat 195.388 kb on + strand, within ygiMat 195.388 kb on + strand, within ygiMat 195.388 kb on + strand, within ygiMat 195.389 kb on - strand, within ygiMat 195.392 kb on + strand, within ygiMat 195.485 kb on + strandat 195.515 kb on + strandat 195.518 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas68
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193,913 + ygiF BNILDI_01005 0.45 -0.8
193,913 + ygiF BNILDI_01005 0.45 +0.8
193,914 - ygiF BNILDI_01005 0.45 +1.2
194,160 + ygiF BNILDI_01005 0.64 -0.3
194,278 + ygiF BNILDI_01005 0.73 -1.3
194,332 + ygiF BNILDI_01005 0.77 +0.1
194,374 - ygiF BNILDI_01005 0.81 -1.1
194,376 - ygiF BNILDI_01005 0.81 -0.0
194,392 + ygiF BNILDI_01005 0.82 -0.5
194,436 - ygiF BNILDI_01005 0.85 -1.1
194,457 + ygiF BNILDI_01005 0.87 +0.9
194,461 + ygiF BNILDI_01005 0.87 +2.0
194,512 - -0.0
194,592 + -0.0
194,598 + -1.6
194,632 + -0.4
194,633 - +2.0
194,633 - -1.2
194,633 - +2.4
194,636 + -1.2
194,644 - -0.5
194,644 - -1.4
194,652 + +1.0
194,652 + +0.4
194,653 - -1.6
194,653 - +0.8
194,675 + +1.2
194,697 - -0.2
194,700 + -1.0
194,701 - +2.1
194,703 + +0.0
194,704 - +0.1
194,828 + +1.0
194,829 - +0.3
194,832 - -0.6
194,892 + +0.4
194,965 + ygiM BNILDI_01010 0.16 +0.4
195,039 + ygiM BNILDI_01010 0.28 +1.4
195,042 + ygiM BNILDI_01010 0.28 +0.2
195,043 - ygiM BNILDI_01010 0.28 -0.6
195,065 + ygiM BNILDI_01010 0.32 -0.6
195,066 - ygiM BNILDI_01010 0.32 -0.6
195,066 - ygiM BNILDI_01010 0.32 -0.1
195,066 - ygiM BNILDI_01010 0.32 +0.2
195,122 - ygiM BNILDI_01010 0.41 +2.1
195,345 + ygiM BNILDI_01010 0.77 -0.7
195,345 + ygiM BNILDI_01010 0.77 -0.3
195,388 + ygiM BNILDI_01010 0.84 -0.1
195,388 + ygiM BNILDI_01010 0.84 -0.2
195,388 + ygiM BNILDI_01010 0.84 -0.6
195,388 + ygiM BNILDI_01010 0.84 -0.5
195,389 - ygiM BNILDI_01010 0.84 -0.3
195,392 + ygiM BNILDI_01010 0.85 +0.1
195,485 + +3.0
195,515 + +0.8
195,518 + +3.0

Or see this region's nucleotide sequence