Experiment: TP7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutQ and eutT overlap by 4 nucleotides eutT and pta overlap by 4 nucleotides pta and eutM overlap by 4 nucleotides eutM and eutN are separated by 105 nucleotides eutN and eutE are separated by 11 nucleotides
HEPCGN_21190: eutQ - ethanolamine utilization acetate kinase EutQ, at 2,993,813 to 2,994,514
eutQ
HEPCGN_21195: eutT - ethanolamine utilization cob(I)yrinic acid a,c-diamide adenosyltransferase EutT, at 2,994,511 to 2,995,314
eutT
HEPCGN_21200: pta - phosphate acetyltransferase, at 2,995,311 to 2,996,327
pta
HEPCGN_21205: eutM - ethanolamine utilization microcompartment protein EutM, at 2,996,324 to 2,996,659
eutM
HEPCGN_21210: eutN - ethanolamine utilization microcompartment protein EutN, at 2,996,765 to 2,997,052
eutN
HEPCGN_21215: eutE - Acetaldehyde dehydrogenase (acetylating) EutE, at 2,997,064 to 2,998,467
eutE
Position (kb)
2995
2996
2997 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2994.336 kb on - strand, within eutQ at 2994.479 kb on + strand at 2994.480 kb on - strand at 2994.480 kb on - strand at 2994.539 kb on + strand at 2994.539 kb on + strand at 2994.955 kb on - strand, within eutT at 2995.185 kb on - strand, within eutT at 2995.187 kb on + strand, within eutT at 2995.188 kb on - strand, within eutT at 2995.234 kb on + strand at 2995.234 kb on + strand at 2995.412 kb on - strand at 2995.667 kb on + strand, within pta at 2995.721 kb on + strand, within pta at 2995.779 kb on + strand, within pta at 2996.083 kb on + strand, within pta at 2996.189 kb on - strand, within pta at 2996.377 kb on - strand, within eutM at 2996.377 kb on - strand, within eutM at 2996.434 kb on + strand, within eutM at 2996.465 kb on - strand, within eutM at 2996.489 kb on - strand, within eutM at 2996.580 kb on + strand, within eutM at 2996.581 kb on - strand, within eutM at 2996.711 kb on - strand at 2996.727 kb on - strand at 2996.765 kb on - strand at 2997.050 kb on + strand at 2997.051 kb on - strand at 2997.078 kb on + strand at 2997.102 kb on - strand at 2997.225 kb on - strand, within eutE at 2997.225 kb on - strand, within eutE at 2997.236 kb on + strand, within eutE at 2997.237 kb on - strand, within eutE
Per-strain Table
Position Strand Gene LocusTag Fraction TP7 remove 2,994,336 - eutQ HEPCGN_21190 0.75 +0.2 2,994,479 + +0.6 2,994,480 - -1.2 2,994,480 - -1.3 2,994,539 + +0.7 2,994,539 + -1.0 2,994,955 - eutT HEPCGN_21195 0.55 -0.4 2,995,185 - eutT HEPCGN_21195 0.84 +2.5 2,995,187 + eutT HEPCGN_21195 0.84 +0.2 2,995,188 - eutT HEPCGN_21195 0.84 +0.9 2,995,234 + +2.1 2,995,234 + -2.2 2,995,412 - +1.5 2,995,667 + pta HEPCGN_21200 0.35 +0.9 2,995,721 + pta HEPCGN_21200 0.40 +0.6 2,995,779 + pta HEPCGN_21200 0.46 +0.2 2,996,083 + pta HEPCGN_21200 0.76 -1.3 2,996,189 - pta HEPCGN_21200 0.86 +2.1 2,996,377 - eutM HEPCGN_21205 0.16 -0.3 2,996,377 - eutM HEPCGN_21205 0.16 +0.5 2,996,434 + eutM HEPCGN_21205 0.33 -1.3 2,996,465 - eutM HEPCGN_21205 0.42 +0.1 2,996,489 - eutM HEPCGN_21205 0.49 -0.2 2,996,580 + eutM HEPCGN_21205 0.76 +0.3 2,996,581 - eutM HEPCGN_21205 0.76 +1.1 2,996,711 - -1.4 2,996,727 - +0.1 2,996,765 - -0.2 2,997,050 + +0.2 2,997,051 - +0.3 2,997,078 + +0.3 2,997,102 - +0.4 2,997,225 - eutE HEPCGN_21215 0.11 -1.0 2,997,225 - eutE HEPCGN_21215 0.11 -1.5 2,997,236 + eutE HEPCGN_21215 0.12 +0.7 2,997,237 - eutE HEPCGN_21215 0.12 +1.3
Or see this region's nucleotide sequence