Experiment: TP7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_11530 and tyrB are separated by 222 nucleotides tyrB and alr are separated by 252 nucleotides
HEPCGN_11530: HEPCGN_11530 - hypothetical protein, at 1,046,165 to 1,046,668
_11530
HEPCGN_11535: tyrB - aromatic amino acid transaminase, at 1,046,891 to 1,048,084
tyrB
HEPCGN_11540: alr - alanine racemase, at 1,048,337 to 1,049,416
alr
Position (kb)
1046
1047
1048
1049 Strain fitness (log2 ratio)
-1
0
1 at 1045.898 kb on - strand at 1045.900 kb on - strand at 1045.900 kb on - strand at 1045.983 kb on - strand at 1046.086 kb on + strand at 1046.205 kb on + strand at 1046.360 kb on - strand, within HEPCGN_11530 at 1046.423 kb on - strand, within HEPCGN_11530 at 1046.495 kb on - strand, within HEPCGN_11530 at 1046.507 kb on + strand, within HEPCGN_11530 at 1046.927 kb on + strand at 1046.928 kb on - strand at 1046.928 kb on - strand at 1047.032 kb on - strand, within tyrB at 1047.099 kb on - strand, within tyrB at 1047.137 kb on - strand, within tyrB at 1047.150 kb on - strand, within tyrB at 1047.235 kb on - strand, within tyrB at 1047.463 kb on + strand, within tyrB at 1047.463 kb on + strand, within tyrB at 1047.509 kb on - strand, within tyrB at 1047.509 kb on - strand, within tyrB at 1047.512 kb on - strand, within tyrB at 1047.563 kb on - strand, within tyrB at 1047.605 kb on - strand, within tyrB at 1047.647 kb on - strand, within tyrB at 1047.681 kb on - strand, within tyrB at 1047.698 kb on - strand, within tyrB at 1047.732 kb on + strand, within tyrB at 1047.733 kb on - strand, within tyrB at 1047.797 kb on + strand, within tyrB at 1047.975 kb on + strand at 1047.976 kb on - strand at 1047.978 kb on + strand at 1047.993 kb on - strand at 1048.117 kb on + strand at 1048.118 kb on - strand at 1048.178 kb on + strand at 1048.179 kb on - strand at 1048.207 kb on + strand at 1048.208 kb on - strand at 1048.208 kb on - strand at 1048.208 kb on - strand at 1048.208 kb on - strand at 1048.233 kb on + strand at 1048.424 kb on + strand at 1048.425 kb on - strand at 1048.488 kb on - strand, within alr at 1048.504 kb on + strand, within alr at 1048.505 kb on - strand, within alr at 1048.505 kb on - strand, within alr at 1048.883 kb on - strand, within alr at 1048.883 kb on - strand, within alr at 1049.039 kb on - strand, within alr
Per-strain Table
Position Strand Gene LocusTag Fraction TP7 remove 1,045,898 - -0.9 1,045,900 - -1.7 1,045,900 - -0.4 1,045,983 - -1.2 1,046,086 + -0.2 1,046,205 + -0.4 1,046,360 - HEPCGN_11530 0.39 +1.1 1,046,423 - HEPCGN_11530 0.51 +0.3 1,046,495 - HEPCGN_11530 0.65 -0.2 1,046,507 + HEPCGN_11530 0.68 +0.4 1,046,927 + +0.9 1,046,928 - -0.3 1,046,928 - -0.8 1,047,032 - tyrB HEPCGN_11535 0.12 +0.8 1,047,099 - tyrB HEPCGN_11535 0.17 +0.3 1,047,137 - tyrB HEPCGN_11535 0.21 -0.2 1,047,150 - tyrB HEPCGN_11535 0.22 +0.6 1,047,235 - tyrB HEPCGN_11535 0.29 +0.6 1,047,463 + tyrB HEPCGN_11535 0.48 +0.7 1,047,463 + tyrB HEPCGN_11535 0.48 -0.8 1,047,509 - tyrB HEPCGN_11535 0.52 +0.2 1,047,509 - tyrB HEPCGN_11535 0.52 -0.2 1,047,512 - tyrB HEPCGN_11535 0.52 -0.4 1,047,563 - tyrB HEPCGN_11535 0.56 -1.3 1,047,605 - tyrB HEPCGN_11535 0.60 -0.4 1,047,647 - tyrB HEPCGN_11535 0.63 +0.4 1,047,681 - tyrB HEPCGN_11535 0.66 +1.2 1,047,698 - tyrB HEPCGN_11535 0.68 -0.9 1,047,732 + tyrB HEPCGN_11535 0.70 -0.6 1,047,733 - tyrB HEPCGN_11535 0.71 +0.6 1,047,797 + tyrB HEPCGN_11535 0.76 +1.0 1,047,975 + -1.4 1,047,976 - -0.3 1,047,978 + +0.2 1,047,993 - -1.6 1,048,117 + -0.2 1,048,118 - -1.2 1,048,178 + +0.6 1,048,179 - -1.2 1,048,207 + -0.2 1,048,208 - -0.3 1,048,208 - -0.4 1,048,208 - +0.8 1,048,208 - -0.9 1,048,233 + +0.3 1,048,424 + +1.2 1,048,425 - -0.2 1,048,488 - alr HEPCGN_11540 0.14 +1.2 1,048,504 + alr HEPCGN_11540 0.15 +1.1 1,048,505 - alr HEPCGN_11540 0.16 +0.8 1,048,505 - alr HEPCGN_11540 0.16 +0.6 1,048,883 - alr HEPCGN_11540 0.51 +0.6 1,048,883 - alr HEPCGN_11540 0.51 +1.7 1,049,039 - alr HEPCGN_11540 0.65 +0.9
Or see this region's nucleotide sequence