Strain Fitness in Escherichia coli ECOR38 around HEPCGN_03215

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisB and hisC overlap by 1 nucleotideshisC and hisD overlap by 4 nucleotides HEPCGN_03210: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 553,547 to 554,614 hisB HEPCGN_03215: hisC - histidinol-phosphate transaminase, at 554,614 to 555,684 hisC HEPCGN_03220: hisD - Histidinol dehydrogenase, at 555,681 to 556,985 hisD Position (kb) 554 555 556Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 553.695 kb on + strand, within hisBat 553.696 kb on - strand, within hisBat 553.759 kb on + strand, within hisBat 553.771 kb on + strand, within hisBat 553.798 kb on + strand, within hisBat 553.880 kb on + strand, within hisBat 553.941 kb on + strand, within hisBat 553.942 kb on - strand, within hisBat 554.103 kb on - strand, within hisBat 554.238 kb on + strand, within hisBat 554.239 kb on - strand, within hisBat 554.245 kb on + strand, within hisBat 554.302 kb on + strand, within hisBat 554.473 kb on - strand, within hisBat 554.496 kb on - strand, within hisBat 554.593 kb on + strandat 554.616 kb on - strandat 554.616 kb on - strandat 554.722 kb on + strand, within hisCat 554.727 kb on + strand, within hisCat 554.789 kb on + strand, within hisCat 554.790 kb on - strand, within hisCat 554.853 kb on - strand, within hisCat 555.125 kb on + strand, within hisCat 555.126 kb on - strand, within hisCat 555.233 kb on + strand, within hisCat 555.233 kb on + strand, within hisCat 555.348 kb on - strand, within hisCat 555.424 kb on - strand, within hisCat 555.467 kb on - strand, within hisCat 555.476 kb on + strand, within hisCat 555.486 kb on - strand, within hisCat 555.544 kb on - strand, within hisCat 555.669 kb on + strandat 555.670 kb on - strandat 555.704 kb on + strandat 555.793 kb on - strandat 555.865 kb on + strand, within hisDat 555.871 kb on + strand, within hisDat 556.292 kb on + strand, within hisDat 556.323 kb on + strand, within hisDat 556.598 kb on - strand, within hisDat 556.598 kb on - strand, within hisDat 556.598 kb on - strand, within hisDat 556.615 kb on - strand, within hisDat 556.641 kb on + strand, within hisDat 556.659 kb on + strand, within hisDat 556.659 kb on + strand, within hisD

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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553,695 + hisB HEPCGN_03210 0.14 -2.9
553,696 - hisB HEPCGN_03210 0.14 -1.4
553,759 + hisB HEPCGN_03210 0.20 +0.3
553,771 + hisB HEPCGN_03210 0.21 -0.5
553,798 + hisB HEPCGN_03210 0.24 +0.0
553,880 + hisB HEPCGN_03210 0.31 -1.4
553,941 + hisB HEPCGN_03210 0.37 -0.4
553,942 - hisB HEPCGN_03210 0.37 -1.1
554,103 - hisB HEPCGN_03210 0.52 -0.2
554,238 + hisB HEPCGN_03210 0.65 -1.2
554,239 - hisB HEPCGN_03210 0.65 +0.3
554,245 + hisB HEPCGN_03210 0.65 +2.4
554,302 + hisB HEPCGN_03210 0.71 -1.4
554,473 - hisB HEPCGN_03210 0.87 +1.1
554,496 - hisB HEPCGN_03210 0.89 +1.0
554,593 + -0.3
554,616 - -0.1
554,616 - +1.9
554,722 + hisC HEPCGN_03215 0.10 -0.8
554,727 + hisC HEPCGN_03215 0.11 +0.9
554,789 + hisC HEPCGN_03215 0.16 -0.3
554,790 - hisC HEPCGN_03215 0.16 -2.2
554,853 - hisC HEPCGN_03215 0.22 -0.6
555,125 + hisC HEPCGN_03215 0.48 +1.0
555,126 - hisC HEPCGN_03215 0.48 -2.9
555,233 + hisC HEPCGN_03215 0.58 -0.1
555,233 + hisC HEPCGN_03215 0.58 -2.2
555,348 - hisC HEPCGN_03215 0.69 -1.7
555,424 - hisC HEPCGN_03215 0.76 -0.2
555,467 - hisC HEPCGN_03215 0.80 -0.5
555,476 + hisC HEPCGN_03215 0.80 -1.2
555,486 - hisC HEPCGN_03215 0.81 +0.6
555,544 - hisC HEPCGN_03215 0.87 +1.3
555,669 + +0.7
555,670 - +1.5
555,704 + +0.9
555,793 - -0.1
555,865 + hisD HEPCGN_03220 0.14 -1.1
555,871 + hisD HEPCGN_03220 0.15 -0.3
556,292 + hisD HEPCGN_03220 0.47 +0.4
556,323 + hisD HEPCGN_03220 0.49 -1.4
556,598 - hisD HEPCGN_03220 0.70 +1.5
556,598 - hisD HEPCGN_03220 0.70 +1.0
556,598 - hisD HEPCGN_03220 0.70 +0.8
556,615 - hisD HEPCGN_03220 0.72 +0.8
556,641 + hisD HEPCGN_03220 0.74 +0.3
556,659 + hisD HEPCGN_03220 0.75 +0.9
556,659 + hisD HEPCGN_03220 0.75 +2.9

Or see this region's nucleotide sequence