Experiment: TP7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dld and bglX are separated by 235 nucleotides bglX and osmF are separated by 251 nucleotides
HEPCGN_02225: dld - D-lactate dehydrogenase, at 368,795 to 370,501
dld
HEPCGN_02230: bglX - beta-glucosidase BglX, at 370,737 to 373,004
bglX
HEPCGN_02235: osmF - glycine betaine ABC transporter substrate-binding protein OsmF, at 373,256 to 374,173
osmF
Position (kb) 370
371
372
373
374 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 369.781 kb on + strand, within dld at 369.784 kb on - strand, within dld at 369.784 kb on - strand, within dld at 369.784 kb on - strand, within dld at 370.072 kb on + strand, within dld at 370.138 kb on - strand, within dld at 370.190 kb on + strand, within dld at 370.191 kb on - strand, within dld at 370.225 kb on + strand, within dld at 370.237 kb on - strand, within dld at 370.297 kb on + strand, within dld at 370.552 kb on + strand at 370.552 kb on + strand at 370.552 kb on + strand at 370.552 kb on + strand at 370.553 kb on - strand at 370.553 kb on - strand at 370.558 kb on + strand at 370.559 kb on - strand at 370.559 kb on - strand at 370.559 kb on - strand at 370.563 kb on - strand at 370.563 kb on - strand at 370.563 kb on - strand at 370.563 kb on - strand at 370.609 kb on + strand at 370.609 kb on + strand at 370.717 kb on + strand at 370.717 kb on + strand at 370.868 kb on - strand at 370.868 kb on - strand at 370.868 kb on - strand at 371.136 kb on + strand, within bglX at 371.136 kb on + strand, within bglX at 371.162 kb on + strand, within bglX at 371.237 kb on + strand, within bglX at 371.237 kb on + strand, within bglX at 371.238 kb on - strand, within bglX at 371.238 kb on - strand, within bglX at 371.238 kb on - strand, within bglX at 371.241 kb on + strand, within bglX at 371.241 kb on + strand, within bglX at 371.241 kb on + strand, within bglX at 371.261 kb on + strand, within bglX at 371.267 kb on + strand, within bglX at 371.267 kb on + strand, within bglX at 371.412 kb on + strand, within bglX at 371.414 kb on + strand, within bglX at 371.414 kb on + strand, within bglX at 371.415 kb on - strand, within bglX at 371.415 kb on - strand, within bglX at 371.547 kb on - strand, within bglX at 371.640 kb on - strand, within bglX at 371.660 kb on + strand, within bglX at 371.661 kb on - strand, within bglX at 371.674 kb on - strand, within bglX at 371.753 kb on - strand, within bglX at 371.762 kb on + strand, within bglX at 371.766 kb on + strand, within bglX at 371.766 kb on + strand, within bglX at 371.771 kb on + strand, within bglX at 371.793 kb on + strand, within bglX at 372.472 kb on - strand, within bglX at 372.532 kb on - strand, within bglX at 372.534 kb on + strand, within bglX at 372.535 kb on - strand, within bglX at 372.561 kb on + strand, within bglX at 372.585 kb on + strand, within bglX at 372.597 kb on + strand, within bglX at 372.648 kb on + strand, within bglX at 372.649 kb on - strand, within bglX at 372.650 kb on + strand, within bglX at 372.651 kb on - strand, within bglX at 372.764 kb on - strand, within bglX at 372.775 kb on - strand, within bglX at 372.885 kb on - strand at 372.922 kb on - strand at 372.922 kb on - strand at 373.131 kb on + strand at 373.140 kb on + strand at 373.145 kb on - strand at 373.148 kb on + strand at 373.215 kb on + strand at 373.427 kb on - strand, within osmF at 373.453 kb on - strand, within osmF at 373.636 kb on + strand, within osmF at 373.637 kb on - strand, within osmF at 373.713 kb on - strand, within osmF at 373.800 kb on - strand, within osmF at 373.979 kb on - strand, within osmF at 373.983 kb on - strand, within osmF
Per-strain Table
Position Strand Gene LocusTag Fraction TP7 remove 369,781 + dld HEPCGN_02225 0.58 -0.2 369,784 - dld HEPCGN_02225 0.58 +1.7 369,784 - dld HEPCGN_02225 0.58 -1.8 369,784 - dld HEPCGN_02225 0.58 +0.3 370,072 + dld HEPCGN_02225 0.75 -0.4 370,138 - dld HEPCGN_02225 0.79 -0.5 370,190 + dld HEPCGN_02225 0.82 -0.1 370,191 - dld HEPCGN_02225 0.82 +0.1 370,225 + dld HEPCGN_02225 0.84 +0.8 370,237 - dld HEPCGN_02225 0.84 +0.0 370,297 + dld HEPCGN_02225 0.88 -1.2 370,552 + +0.2 370,552 + -0.1 370,552 + -1.1 370,552 + -0.7 370,553 - +0.4 370,553 - -1.6 370,558 + +0.7 370,559 - +0.8 370,559 - +0.4 370,559 - -1.8 370,563 - -0.1 370,563 - +0.7 370,563 - -1.0 370,563 - -0.5 370,609 + -0.3 370,609 + -1.7 370,717 + -0.6 370,717 + -2.9 370,868 - -1.6 370,868 - -2.0 370,868 - -1.8 371,136 + bglX HEPCGN_02230 0.18 +0.0 371,136 + bglX HEPCGN_02230 0.18 -1.0 371,162 + bglX HEPCGN_02230 0.19 -0.7 371,237 + bglX HEPCGN_02230 0.22 -1.1 371,237 + bglX HEPCGN_02230 0.22 -1.2 371,238 - bglX HEPCGN_02230 0.22 -1.8 371,238 - bglX HEPCGN_02230 0.22 -2.1 371,238 - bglX HEPCGN_02230 0.22 -1.8 371,241 + bglX HEPCGN_02230 0.22 -1.0 371,241 + bglX HEPCGN_02230 0.22 -0.3 371,241 + bglX HEPCGN_02230 0.22 -0.8 371,261 + bglX HEPCGN_02230 0.23 -2.2 371,267 + bglX HEPCGN_02230 0.23 -0.8 371,267 + bglX HEPCGN_02230 0.23 -2.6 371,412 + bglX HEPCGN_02230 0.30 -0.7 371,414 + bglX HEPCGN_02230 0.30 +1.0 371,414 + bglX HEPCGN_02230 0.30 +0.2 371,415 - bglX HEPCGN_02230 0.30 -2.5 371,415 - bglX HEPCGN_02230 0.30 -1.0 371,547 - bglX HEPCGN_02230 0.36 -2.4 371,640 - bglX HEPCGN_02230 0.40 -1.0 371,660 + bglX HEPCGN_02230 0.41 +0.0 371,661 - bglX HEPCGN_02230 0.41 -3.0 371,674 - bglX HEPCGN_02230 0.41 -1.8 371,753 - bglX HEPCGN_02230 0.45 -1.4 371,762 + bglX HEPCGN_02230 0.45 -2.7 371,766 + bglX HEPCGN_02230 0.45 -1.7 371,766 + bglX HEPCGN_02230 0.45 -1.7 371,771 + bglX HEPCGN_02230 0.46 -0.8 371,793 + bglX HEPCGN_02230 0.47 -1.1 372,472 - bglX HEPCGN_02230 0.76 -1.0 372,532 - bglX HEPCGN_02230 0.79 -1.0 372,534 + bglX HEPCGN_02230 0.79 -2.3 372,535 - bglX HEPCGN_02230 0.79 -0.7 372,561 + bglX HEPCGN_02230 0.80 +1.0 372,585 + bglX HEPCGN_02230 0.81 +1.0 372,597 + bglX HEPCGN_02230 0.82 -2.4 372,648 + bglX HEPCGN_02230 0.84 -1.2 372,649 - bglX HEPCGN_02230 0.84 -0.8 372,650 + bglX HEPCGN_02230 0.84 -2.3 372,651 - bglX HEPCGN_02230 0.84 -1.3 372,764 - bglX HEPCGN_02230 0.89 -0.7 372,775 - bglX HEPCGN_02230 0.90 -1.7 372,885 - +0.0 372,922 - -0.3 372,922 - -1.7 373,131 + +0.0 373,140 + +0.9 373,145 - -0.9 373,148 + -0.1 373,215 + -0.4 373,427 - osmF HEPCGN_02235 0.19 +0.7 373,453 - osmF HEPCGN_02235 0.21 +0.0 373,636 + osmF HEPCGN_02235 0.41 +0.7 373,637 - osmF HEPCGN_02235 0.42 +0.5 373,713 - osmF HEPCGN_02235 0.50 +0.6 373,800 - osmF HEPCGN_02235 0.59 -0.9 373,979 - osmF HEPCGN_02235 0.79 +0.0 373,983 - osmF HEPCGN_02235 0.79 +0.5
Or see this region's nucleotide sequence