Experiment: TP7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiD and ecdD are separated by 10 nucleotides ecdD and pphB are separated by 41 nucleotides pphB and mutS are separated by 105 nucleotides
HEPCGN_19690: ubiD - phenolic acid decarboxylase, at 2,693,558 to 2,694,985
ubiD
HEPCGN_19695: ecdD - Protein EcdD, at 2,694,996 to 2,695,232
ecdD
HEPCGN_19700: pphB - protein-serine/threonine phosphatase, at 2,695,274 to 2,695,930
pphB
HEPCGN_19705: mutS - DNA mismatch repair protein MutS, at 2,696,036 to 2,698,597
mutS
Position (kb)
2694
2695
2696 Strain fitness (log2 ratio)
-2
-1
0
1 at 2694.050 kb on + strand, within ubiD at 2694.077 kb on - strand, within ubiD at 2694.350 kb on - strand, within ubiD at 2694.584 kb on + strand, within ubiD at 2694.585 kb on - strand, within ubiD at 2694.585 kb on - strand, within ubiD at 2694.703 kb on + strand, within ubiD at 2694.802 kb on + strand, within ubiD at 2694.803 kb on - strand, within ubiD at 2694.906 kb on - strand at 2694.983 kb on + strand at 2695.071 kb on + strand, within ecdD at 2695.092 kb on + strand, within ecdD at 2695.092 kb on + strand, within ecdD at 2695.093 kb on - strand, within ecdD at 2695.094 kb on + strand, within ecdD at 2695.095 kb on - strand, within ecdD at 2695.095 kb on - strand, within ecdD at 2695.095 kb on - strand, within ecdD at 2695.224 kb on - strand at 2695.231 kb on - strand at 2695.330 kb on - strand at 2695.370 kb on - strand, within pphB at 2695.750 kb on + strand, within pphB at 2695.792 kb on + strand, within pphB at 2695.865 kb on - strand at 2695.882 kb on + strand at 2695.962 kb on - strand at 2695.964 kb on - strand at 2696.175 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TP7 remove 2,694,050 + ubiD HEPCGN_19690 0.34 +0.5 2,694,077 - ubiD HEPCGN_19690 0.36 -0.2 2,694,350 - ubiD HEPCGN_19690 0.55 +0.1 2,694,584 + ubiD HEPCGN_19690 0.72 -0.5 2,694,585 - ubiD HEPCGN_19690 0.72 -0.8 2,694,585 - ubiD HEPCGN_19690 0.72 -0.8 2,694,703 + ubiD HEPCGN_19690 0.80 -0.3 2,694,802 + ubiD HEPCGN_19690 0.87 +0.3 2,694,803 - ubiD HEPCGN_19690 0.87 +0.2 2,694,906 - +0.5 2,694,983 + +0.5 2,695,071 + ecdD HEPCGN_19695 0.32 +0.5 2,695,092 + ecdD HEPCGN_19695 0.41 -0.5 2,695,092 + ecdD HEPCGN_19695 0.41 +0.8 2,695,093 - ecdD HEPCGN_19695 0.41 +0.2 2,695,094 + ecdD HEPCGN_19695 0.41 +0.1 2,695,095 - ecdD HEPCGN_19695 0.42 -0.0 2,695,095 - ecdD HEPCGN_19695 0.42 -1.0 2,695,095 - ecdD HEPCGN_19695 0.42 +0.0 2,695,224 - -0.3 2,695,231 - +0.1 2,695,330 - -0.5 2,695,370 - pphB HEPCGN_19700 0.15 +0.5 2,695,750 + pphB HEPCGN_19700 0.72 -0.0 2,695,792 + pphB HEPCGN_19700 0.79 -1.9 2,695,865 - -0.6 2,695,882 + +0.5 2,695,962 - +1.6 2,695,964 - -2.5 2,696,175 - -1.7
Or see this region's nucleotide sequence