Experiment: TP7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dprA and smg overlap by 29 nucleotides smg and yrdD are separated by 28 nucleotides yrdD and tsaC are separated by 4 nucleotides tsaC and aroE are separated by 4 nucleotides
HEPCGN_16365: dprA - DNA-protecting protein DprA, at 2,025,115 to 2,026,239
dprA
HEPCGN_16370: smg - DUF494 domain-containing protein, at 2,026,211 to 2,026,684
smg
HEPCGN_16375: yrdD - Uncharacterized protein YrdD, at 2,026,713 to 2,027,255
yrdD
HEPCGN_16380: tsaC - L-threonylcarbamoyladenylate synthase type 1 TsaC, at 2,027,260 to 2,027,832
tsaC
HEPCGN_16385: aroE - shikimate dehydrogenase, at 2,027,837 to 2,028,655
aroE
Position (kb)
2026
2027
2028 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2025.767 kb on + strand, within dprA at 2025.767 kb on + strand, within dprA at 2025.767 kb on + strand, within dprA at 2025.768 kb on - strand, within dprA at 2025.771 kb on + strand, within dprA at 2025.772 kb on - strand, within dprA at 2025.779 kb on + strand, within dprA at 2025.779 kb on + strand, within dprA at 2025.779 kb on + strand, within dprA at 2025.779 kb on + strand, within dprA at 2025.780 kb on - strand, within dprA at 2025.780 kb on - strand, within dprA at 2025.911 kb on + strand, within dprA at 2025.911 kb on + strand, within dprA at 2025.912 kb on - strand, within dprA at 2025.912 kb on - strand, within dprA at 2026.009 kb on + strand, within dprA at 2026.064 kb on + strand, within dprA at 2026.065 kb on - strand, within dprA at 2026.065 kb on - strand, within dprA at 2026.077 kb on - strand, within dprA at 2026.127 kb on + strand at 2026.127 kb on + strand at 2026.127 kb on + strand at 2026.128 kb on - strand at 2026.130 kb on + strand at 2026.130 kb on + strand at 2026.154 kb on + strand at 2026.186 kb on + strand at 2026.187 kb on - strand at 2026.187 kb on - strand at 2026.221 kb on + strand at 2026.222 kb on - strand at 2026.224 kb on + strand at 2026.225 kb on - strand at 2026.230 kb on - strand at 2026.230 kb on - strand at 2026.230 kb on - strand at 2026.245 kb on - strand at 2026.247 kb on - strand at 2026.327 kb on + strand, within smg at 2026.331 kb on + strand, within smg at 2026.331 kb on + strand, within smg at 2026.331 kb on + strand, within smg at 2026.332 kb on - strand, within smg at 2026.332 kb on - strand, within smg at 2026.332 kb on - strand, within smg at 2026.332 kb on - strand, within smg at 2026.332 kb on - strand, within smg at 2026.423 kb on + strand, within smg at 2026.424 kb on - strand, within smg at 2026.427 kb on + strand, within smg at 2026.427 kb on + strand, within smg at 2026.428 kb on - strand, within smg at 2026.428 kb on - strand, within smg at 2026.428 kb on - strand, within smg at 2026.505 kb on - strand, within smg at 2026.672 kb on + strand at 2026.704 kb on + strand at 2026.704 kb on + strand at 2026.742 kb on + strand at 2026.742 kb on + strand at 2026.825 kb on - strand, within yrdD at 2026.839 kb on + strand, within yrdD at 2026.874 kb on + strand, within yrdD at 2026.930 kb on + strand, within yrdD at 2026.967 kb on + strand, within yrdD at 2026.972 kb on - strand, within yrdD at 2026.988 kb on + strand, within yrdD at 2027.003 kb on + strand, within yrdD at 2027.024 kb on + strand, within yrdD at 2027.024 kb on + strand, within yrdD at 2027.024 kb on + strand, within yrdD at 2027.024 kb on + strand, within yrdD at 2027.024 kb on + strand, within yrdD at 2027.204 kb on + strand at 2027.256 kb on + strand at 2027.256 kb on + strand at 2027.835 kb on + strand at 2027.835 kb on + strand at 2027.836 kb on - strand at 2027.836 kb on - strand at 2027.846 kb on + strand at 2027.846 kb on + strand at 2027.846 kb on + strand at 2027.847 kb on - strand at 2027.989 kb on + strand, within aroE at 2027.989 kb on + strand, within aroE at 2027.990 kb on - strand, within aroE at 2028.001 kb on + strand, within aroE at 2028.001 kb on + strand, within aroE at 2028.028 kb on + strand, within aroE at 2028.136 kb on + strand, within aroE at 2028.145 kb on + strand, within aroE at 2028.146 kb on - strand, within aroE at 2028.148 kb on - strand, within aroE at 2028.237 kb on + strand, within aroE at 2028.240 kb on + strand, within aroE at 2028.240 kb on + strand, within aroE at 2028.241 kb on - strand, within aroE
Per-strain Table
Position Strand Gene LocusTag Fraction TP7 remove 2,025,767 + dprA HEPCGN_16365 0.58 -1.0 2,025,767 + dprA HEPCGN_16365 0.58 -0.5 2,025,767 + dprA HEPCGN_16365 0.58 -0.1 2,025,768 - dprA HEPCGN_16365 0.58 -1.0 2,025,771 + dprA HEPCGN_16365 0.58 -0.9 2,025,772 - dprA HEPCGN_16365 0.58 -2.6 2,025,779 + dprA HEPCGN_16365 0.59 +1.1 2,025,779 + dprA HEPCGN_16365 0.59 -0.5 2,025,779 + dprA HEPCGN_16365 0.59 -1.3 2,025,779 + dprA HEPCGN_16365 0.59 -2.6 2,025,780 - dprA HEPCGN_16365 0.59 +0.6 2,025,780 - dprA HEPCGN_16365 0.59 +0.4 2,025,911 + dprA HEPCGN_16365 0.71 +0.4 2,025,911 + dprA HEPCGN_16365 0.71 -0.5 2,025,912 - dprA HEPCGN_16365 0.71 +0.8 2,025,912 - dprA HEPCGN_16365 0.71 +0.7 2,026,009 + dprA HEPCGN_16365 0.79 +0.7 2,026,064 + dprA HEPCGN_16365 0.84 +0.8 2,026,065 - dprA HEPCGN_16365 0.84 -0.3 2,026,065 - dprA HEPCGN_16365 0.84 -0.2 2,026,077 - dprA HEPCGN_16365 0.86 +0.3 2,026,127 + +0.3 2,026,127 + +0.3 2,026,127 + -0.3 2,026,128 - -0.6 2,026,130 + -0.1 2,026,130 + -0.1 2,026,154 + -0.3 2,026,186 + -1.0 2,026,187 - -1.2 2,026,187 - +1.0 2,026,221 + +0.3 2,026,222 - +2.3 2,026,224 + -0.4 2,026,225 - -1.0 2,026,230 - -2.1 2,026,230 - -0.1 2,026,230 - +0.7 2,026,245 - -0.2 2,026,247 - -1.1 2,026,327 + smg HEPCGN_16370 0.24 +0.7 2,026,331 + smg HEPCGN_16370 0.25 +0.6 2,026,331 + smg HEPCGN_16370 0.25 -3.1 2,026,331 + smg HEPCGN_16370 0.25 -0.1 2,026,332 - smg HEPCGN_16370 0.26 -0.3 2,026,332 - smg HEPCGN_16370 0.26 -1.7 2,026,332 - smg HEPCGN_16370 0.26 +0.0 2,026,332 - smg HEPCGN_16370 0.26 -1.8 2,026,332 - smg HEPCGN_16370 0.26 -0.2 2,026,423 + smg HEPCGN_16370 0.45 -0.8 2,026,424 - smg HEPCGN_16370 0.45 +0.3 2,026,427 + smg HEPCGN_16370 0.46 +0.1 2,026,427 + smg HEPCGN_16370 0.46 +1.3 2,026,428 - smg HEPCGN_16370 0.46 +0.7 2,026,428 - smg HEPCGN_16370 0.46 -0.3 2,026,428 - smg HEPCGN_16370 0.46 +0.6 2,026,505 - smg HEPCGN_16370 0.62 +2.0 2,026,672 + +0.7 2,026,704 + -0.3 2,026,704 + -0.3 2,026,742 + +0.9 2,026,742 + +1.2 2,026,825 - yrdD HEPCGN_16375 0.21 +1.2 2,026,839 + yrdD HEPCGN_16375 0.23 +2.4 2,026,874 + yrdD HEPCGN_16375 0.30 +0.8 2,026,930 + yrdD HEPCGN_16375 0.40 +2.0 2,026,967 + yrdD HEPCGN_16375 0.47 +2.7 2,026,972 - yrdD HEPCGN_16375 0.48 +2.2 2,026,988 + yrdD HEPCGN_16375 0.51 +0.8 2,027,003 + yrdD HEPCGN_16375 0.53 +0.8 2,027,024 + yrdD HEPCGN_16375 0.57 +4.9 2,027,024 + yrdD HEPCGN_16375 0.57 +2.1 2,027,024 + yrdD HEPCGN_16375 0.57 +1.5 2,027,024 + yrdD HEPCGN_16375 0.57 -0.1 2,027,024 + yrdD HEPCGN_16375 0.57 +1.6 2,027,204 + +1.2 2,027,256 + +1.2 2,027,256 + -0.3 2,027,835 + +0.8 2,027,835 + +0.3 2,027,836 - -1.9 2,027,836 - +2.0 2,027,846 + -0.2 2,027,846 + -0.3 2,027,846 + -2.0 2,027,847 - +0.2 2,027,989 + aroE HEPCGN_16385 0.19 +0.2 2,027,989 + aroE HEPCGN_16385 0.19 -1.7 2,027,990 - aroE HEPCGN_16385 0.19 -2.3 2,028,001 + aroE HEPCGN_16385 0.20 -1.0 2,028,001 + aroE HEPCGN_16385 0.20 -2.1 2,028,028 + aroE HEPCGN_16385 0.23 -1.9 2,028,136 + aroE HEPCGN_16385 0.37 -2.9 2,028,145 + aroE HEPCGN_16385 0.38 -2.6 2,028,146 - aroE HEPCGN_16385 0.38 -1.2 2,028,148 - aroE HEPCGN_16385 0.38 -3.1 2,028,237 + aroE HEPCGN_16385 0.49 -1.8 2,028,240 + aroE HEPCGN_16385 0.49 -1.5 2,028,240 + aroE HEPCGN_16385 0.49 -1.1 2,028,241 - aroE HEPCGN_16385 0.49 -1.6
Or see this region's nucleotide sequence