Strain Fitness in Escherichia coli ECOR38 around HEPCGN_07410

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmhpC and mhpB are separated by 17 nucleotidesmhpB and mhpA are separated by 1 nucleotidesmhpA and mhpR are separated by 190 nucleotides HEPCGN_07400: mhpC - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase, at 213,774 to 214,640 mhpC HEPCGN_07405: mhpB - 3-carboxyethylcatechol 2,3-dioxygenase, at 214,658 to 215,602 mhpB HEPCGN_07410: mhpA - bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, at 215,604 to 217,268 mhpA HEPCGN_07415: mhpR - DNA-binding transcriptional regulator, at 217,459 to 218,292 mhpR Position (kb) 215 216 217 218Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 214.655 kb on + strandat 214.719 kb on + strandat 214.720 kb on - strandat 214.853 kb on + strand, within mhpBat 214.854 kb on - strand, within mhpBat 215.272 kb on + strand, within mhpBat 215.273 kb on - strand, within mhpBat 215.273 kb on - strand, within mhpBat 215.273 kb on - strand, within mhpBat 215.273 kb on - strand, within mhpBat 215.273 kb on - strand, within mhpBat 215.428 kb on - strand, within mhpBat 215.443 kb on - strand, within mhpBat 215.534 kb on + strandat 215.557 kb on - strandat 215.592 kb on + strandat 215.592 kb on + strandat 215.593 kb on - strandat 215.593 kb on - strandat 215.669 kb on + strandat 215.670 kb on - strandat 215.678 kb on + strandat 215.678 kb on + strandat 215.679 kb on - strandat 215.679 kb on - strandat 215.681 kb on + strandat 215.799 kb on + strand, within mhpAat 215.803 kb on - strand, within mhpAat 215.803 kb on - strand, within mhpAat 216.109 kb on + strand, within mhpAat 216.110 kb on - strand, within mhpAat 216.130 kb on + strand, within mhpAat 216.159 kb on + strand, within mhpAat 216.164 kb on + strand, within mhpAat 216.165 kb on - strand, within mhpAat 216.256 kb on + strand, within mhpAat 216.257 kb on - strand, within mhpAat 216.257 kb on - strand, within mhpAat 216.257 kb on - strand, within mhpAat 216.257 kb on - strand, within mhpAat 216.347 kb on - strand, within mhpAat 216.347 kb on - strand, within mhpAat 216.446 kb on - strand, within mhpAat 216.512 kb on + strand, within mhpAat 216.561 kb on - strand, within mhpAat 216.631 kb on + strand, within mhpAat 216.631 kb on + strand, within mhpAat 216.631 kb on + strand, within mhpAat 216.632 kb on - strand, within mhpAat 216.632 kb on - strand, within mhpAat 216.632 kb on - strand, within mhpAat 216.635 kb on + strand, within mhpAat 216.636 kb on - strand, within mhpAat 216.975 kb on - strand, within mhpAat 217.029 kb on - strand, within mhpAat 217.125 kb on - strandat 217.171 kb on + strandat 217.226 kb on + strandat 217.260 kb on + strandat 217.260 kb on + strandat 217.312 kb on + strandat 217.371 kb on - strandat 217.436 kb on + strandat 217.636 kb on + strand, within mhpRat 217.636 kb on + strand, within mhpRat 217.637 kb on - strand, within mhpRat 217.695 kb on - strand, within mhpRat 218.059 kb on + strand, within mhpRat 218.060 kb on - strand, within mhpRat 218.095 kb on - strand, within mhpRat 218.149 kb on + strand, within mhpRat 218.149 kb on + strand, within mhpRat 218.149 kb on + strand, within mhpRat 218.150 kb on - strand, within mhpRat 218.150 kb on - strand, within mhpRat 218.150 kb on - strand, within mhpRat 218.251 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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214,655 + +1.0
214,719 + +0.0
214,720 - -0.1
214,853 + mhpB HEPCGN_07405 0.21 +0.7
214,854 - mhpB HEPCGN_07405 0.21 -1.1
215,272 + mhpB HEPCGN_07405 0.65 -0.3
215,273 - mhpB HEPCGN_07405 0.65 -1.9
215,273 - mhpB HEPCGN_07405 0.65 -1.2
215,273 - mhpB HEPCGN_07405 0.65 +0.7
215,273 - mhpB HEPCGN_07405 0.65 -0.4
215,273 - mhpB HEPCGN_07405 0.65 -0.7
215,428 - mhpB HEPCGN_07405 0.81 -0.1
215,443 - mhpB HEPCGN_07405 0.83 -0.7
215,534 + +1.5
215,557 - -2.3
215,592 + -1.7
215,592 + +0.2
215,593 - -1.3
215,593 - +0.5
215,669 + +1.3
215,670 - -1.7
215,678 + +1.0
215,678 + +1.1
215,679 - -0.8
215,679 - +1.6
215,681 + -1.0
215,799 + mhpA HEPCGN_07410 0.12 +1.0
215,803 - mhpA HEPCGN_07410 0.12 +0.5
215,803 - mhpA HEPCGN_07410 0.12 +0.8
216,109 + mhpA HEPCGN_07410 0.30 -1.2
216,110 - mhpA HEPCGN_07410 0.30 -0.9
216,130 + mhpA HEPCGN_07410 0.32 -1.4
216,159 + mhpA HEPCGN_07410 0.33 +1.2
216,164 + mhpA HEPCGN_07410 0.34 -0.0
216,165 - mhpA HEPCGN_07410 0.34 +0.2
216,256 + mhpA HEPCGN_07410 0.39 -0.6
216,257 - mhpA HEPCGN_07410 0.39 -0.9
216,257 - mhpA HEPCGN_07410 0.39 +1.2
216,257 - mhpA HEPCGN_07410 0.39 +0.3
216,257 - mhpA HEPCGN_07410 0.39 -0.3
216,347 - mhpA HEPCGN_07410 0.45 +0.1
216,347 - mhpA HEPCGN_07410 0.45 -3.4
216,446 - mhpA HEPCGN_07410 0.51 +1.5
216,512 + mhpA HEPCGN_07410 0.55 +0.6
216,561 - mhpA HEPCGN_07410 0.57 -0.9
216,631 + mhpA HEPCGN_07410 0.62 +1.3
216,631 + mhpA HEPCGN_07410 0.62 +0.8
216,631 + mhpA HEPCGN_07410 0.62 -2.5
216,632 - mhpA HEPCGN_07410 0.62 -2.5
216,632 - mhpA HEPCGN_07410 0.62 -1.4
216,632 - mhpA HEPCGN_07410 0.62 +0.2
216,635 + mhpA HEPCGN_07410 0.62 -0.4
216,636 - mhpA HEPCGN_07410 0.62 +0.4
216,975 - mhpA HEPCGN_07410 0.82 -0.1
217,029 - mhpA HEPCGN_07410 0.86 -0.2
217,125 - -0.0
217,171 + -2.3
217,226 + +1.8
217,260 + -1.0
217,260 + -0.3
217,312 + -0.4
217,371 - +0.7
217,436 + -0.2
217,636 + mhpR HEPCGN_07415 0.21 -0.4
217,636 + mhpR HEPCGN_07415 0.21 -1.1
217,637 - mhpR HEPCGN_07415 0.21 +0.2
217,695 - mhpR HEPCGN_07415 0.28 -1.7
218,059 + mhpR HEPCGN_07415 0.72 -2.5
218,060 - mhpR HEPCGN_07415 0.72 +0.2
218,095 - mhpR HEPCGN_07415 0.76 +1.3
218,149 + mhpR HEPCGN_07415 0.83 +0.2
218,149 + mhpR HEPCGN_07415 0.83 -1.9
218,149 + mhpR HEPCGN_07415 0.83 +1.0
218,150 - mhpR HEPCGN_07415 0.83 +0.8
218,150 - mhpR HEPCGN_07415 0.83 -2.2
218,150 - mhpR HEPCGN_07415 0.83 +0.1
218,251 - +0.3

Or see this region's nucleotide sequence