Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clsA and kch are separated by 370 nucleotides kch and yciI are separated by 299 nucleotides yciI and tonB are separated by 223 nucleotides
HEPCGN_25125: clsA - cardiolipin synthase ClsA, at 3,779,229 to 3,780,689
clsA
HEPCGN_25130: kch - voltage-gated potassium channel protein, at 3,781,060 to 3,782,313
kch
HEPCGN_25135: yciI - YciI family protein, at 3,782,613 to 3,782,909
yciI
HEPCGN_25140: tonB - TonB system transport protein TonB, at 3,783,133 to 3,783,849
tonB
Position (kb)
3781
3782
3783 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3780.174 kb on - strand, within clsA at 3780.327 kb on + strand, within clsA at 3780.402 kb on - strand, within clsA at 3780.420 kb on - strand, within clsA at 3780.420 kb on - strand, within clsA at 3780.425 kb on + strand, within clsA at 3780.435 kb on - strand, within clsA at 3780.502 kb on - strand, within clsA at 3780.556 kb on + strand at 3780.561 kb on - strand at 3780.607 kb on - strand at 3780.622 kb on - strand at 3780.643 kb on + strand at 3780.675 kb on - strand at 3780.689 kb on + strand at 3780.796 kb on - strand at 3780.820 kb on - strand at 3780.824 kb on + strand at 3780.825 kb on - strand at 3780.927 kb on + strand at 3780.927 kb on + strand at 3780.972 kb on + strand at 3780.994 kb on + strand at 3781.000 kb on - strand at 3781.035 kb on - strand at 3781.165 kb on - strand at 3781.184 kb on - strand at 3781.244 kb on - strand, within kch at 3781.599 kb on + strand, within kch at 3781.599 kb on + strand, within kch at 3781.600 kb on - strand, within kch at 3781.600 kb on - strand, within kch at 3781.665 kb on + strand, within kch at 3781.665 kb on + strand, within kch at 3781.665 kb on + strand, within kch at 3781.666 kb on - strand, within kch at 3781.666 kb on - strand, within kch at 3781.666 kb on - strand, within kch at 3781.666 kb on - strand, within kch at 3781.666 kb on - strand, within kch at 3781.707 kb on + strand, within kch at 3781.937 kb on - strand, within kch at 3781.957 kb on - strand, within kch at 3781.957 kb on - strand, within kch at 3782.009 kb on + strand, within kch at 3782.071 kb on + strand, within kch at 3782.113 kb on + strand, within kch at 3782.298 kb on + strand at 3782.341 kb on + strand at 3782.372 kb on + strand at 3782.401 kb on + strand at 3782.404 kb on + strand at 3782.404 kb on + strand at 3782.491 kb on - strand at 3782.491 kb on - strand at 3782.501 kb on - strand at 3782.512 kb on + strand at 3782.512 kb on + strand at 3782.512 kb on + strand at 3782.513 kb on - strand at 3782.515 kb on + strand at 3782.549 kb on + strand at 3782.550 kb on - strand at 3782.611 kb on - strand at 3782.627 kb on + strand at 3782.667 kb on + strand, within yciI at 3782.820 kb on + strand, within yciI at 3782.820 kb on + strand, within yciI at 3782.821 kb on - strand, within yciI at 3782.821 kb on - strand, within yciI at 3782.826 kb on + strand, within yciI at 3782.893 kb on + strand at 3782.897 kb on + strand at 3782.898 kb on - strand at 3782.938 kb on + strand at 3782.943 kb on + strand at 3782.943 kb on + strand at 3783.123 kb on + strand at 3783.218 kb on - strand, within tonB at 3783.245 kb on + strand, within tonB at 3783.245 kb on + strand, within tonB at 3783.245 kb on + strand, within tonB
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 3,780,174 - clsA HEPCGN_25125 0.65 -1.6 3,780,327 + clsA HEPCGN_25125 0.75 -0.9 3,780,402 - clsA HEPCGN_25125 0.80 +0.3 3,780,420 - clsA HEPCGN_25125 0.82 -2.9 3,780,420 - clsA HEPCGN_25125 0.82 +2.6 3,780,425 + clsA HEPCGN_25125 0.82 -1.6 3,780,435 - clsA HEPCGN_25125 0.83 -1.6 3,780,502 - clsA HEPCGN_25125 0.87 -0.7 3,780,556 + -1.0 3,780,561 - -1.4 3,780,607 - -1.6 3,780,622 - -0.4 3,780,643 + -2.8 3,780,675 - -2.1 3,780,689 + -1.0 3,780,796 - -0.3 3,780,820 - +0.9 3,780,824 + -1.2 3,780,825 - +0.1 3,780,927 + +0.1 3,780,927 + +0.4 3,780,972 + -0.1 3,780,994 + +1.6 3,781,000 - -1.4 3,781,035 - -0.5 3,781,165 - -2.0 3,781,184 - +0.6 3,781,244 - kch HEPCGN_25130 0.15 -0.2 3,781,599 + kch HEPCGN_25130 0.43 -1.2 3,781,599 + kch HEPCGN_25130 0.43 +0.8 3,781,600 - kch HEPCGN_25130 0.43 +1.0 3,781,600 - kch HEPCGN_25130 0.43 +0.2 3,781,665 + kch HEPCGN_25130 0.48 +1.1 3,781,665 + kch HEPCGN_25130 0.48 -0.4 3,781,665 + kch HEPCGN_25130 0.48 +0.4 3,781,666 - kch HEPCGN_25130 0.48 +0.1 3,781,666 - kch HEPCGN_25130 0.48 +1.9 3,781,666 - kch HEPCGN_25130 0.48 +1.1 3,781,666 - kch HEPCGN_25130 0.48 +0.4 3,781,666 - kch HEPCGN_25130 0.48 +0.6 3,781,707 + kch HEPCGN_25130 0.52 -1.3 3,781,937 - kch HEPCGN_25130 0.70 -0.2 3,781,957 - kch HEPCGN_25130 0.72 +1.0 3,781,957 - kch HEPCGN_25130 0.72 -1.0 3,782,009 + kch HEPCGN_25130 0.76 +0.6 3,782,071 + kch HEPCGN_25130 0.81 +0.1 3,782,113 + kch HEPCGN_25130 0.84 -0.0 3,782,298 + +0.2 3,782,341 + +0.3 3,782,372 + +0.4 3,782,401 + +0.5 3,782,404 + +0.3 3,782,404 + -0.0 3,782,491 - -1.3 3,782,491 - -0.6 3,782,501 - +1.6 3,782,512 + -0.2 3,782,512 + +0.5 3,782,512 + +0.1 3,782,513 - -0.1 3,782,515 + +1.6 3,782,549 + +1.0 3,782,550 - +0.3 3,782,611 - +0.1 3,782,627 + -0.0 3,782,667 + yciI HEPCGN_25135 0.18 +0.4 3,782,820 + yciI HEPCGN_25135 0.70 +0.2 3,782,820 + yciI HEPCGN_25135 0.70 -0.0 3,782,821 - yciI HEPCGN_25135 0.70 -0.7 3,782,821 - yciI HEPCGN_25135 0.70 -1.0 3,782,826 + yciI HEPCGN_25135 0.72 -0.0 3,782,893 + -0.3 3,782,897 + +0.2 3,782,898 - +0.1 3,782,938 + -0.9 3,782,943 + +1.3 3,782,943 + +0.0 3,783,123 + -0.5 3,783,218 - tonB HEPCGN_25140 0.12 +1.6 3,783,245 + tonB HEPCGN_25140 0.16 -0.7 3,783,245 + tonB HEPCGN_25140 0.16 -0.0 3,783,245 + tonB HEPCGN_25140 0.16 -1.7
Or see this region's nucleotide sequence