Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_24785 and HEPCGN_24790 are separated by 338 nucleotides HEPCGN_24790 and nhaP2 are separated by 119 nucleotides nhaP2 and ldcA are separated by 94 nucleotides
HEPCGN_24785: HEPCGN_24785 - hypothetical protein, at 3,710,266 to 3,711,108
_24785
HEPCGN_24790: HEPCGN_24790 - hypothetical protein, at 3,711,447 to 3,711,608
_24790
HEPCGN_24795: nhaP2 - potassium/proton antiporter, at 3,711,728 to 3,713,464
nhaP2
HEPCGN_24800: ldcA - muramoyltetrapeptide carboxypeptidase, at 3,713,559 to 3,714,473
ldcA
Position (kb)
3711
3712
3713
3714 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3710.798 kb on - strand, within HEPCGN_24785 at 3710.800 kb on - strand, within HEPCGN_24785 at 3710.839 kb on - strand, within HEPCGN_24785 at 3710.839 kb on - strand, within HEPCGN_24785 at 3710.839 kb on - strand, within HEPCGN_24785 at 3710.893 kb on + strand, within HEPCGN_24785 at 3710.893 kb on + strand, within HEPCGN_24785 at 3710.917 kb on - strand, within HEPCGN_24785 at 3710.972 kb on + strand, within HEPCGN_24785 at 3711.097 kb on - strand at 3711.114 kb on + strand at 3711.114 kb on + strand at 3711.119 kb on - strand at 3711.119 kb on - strand at 3711.129 kb on + strand at 3711.130 kb on - strand at 3711.204 kb on - strand at 3711.204 kb on - strand at 3711.205 kb on + strand at 3711.206 kb on - strand at 3711.206 kb on - strand at 3711.206 kb on - strand at 3711.206 kb on - strand at 3711.210 kb on - strand at 3711.210 kb on - strand at 3711.259 kb on - strand at 3711.259 kb on - strand at 3711.267 kb on + strand at 3711.267 kb on + strand at 3711.282 kb on - strand at 3711.282 kb on - strand at 3711.293 kb on - strand at 3711.293 kb on - strand at 3711.296 kb on - strand at 3711.305 kb on - strand at 3711.353 kb on + strand at 3711.401 kb on - strand at 3711.412 kb on + strand at 3711.488 kb on + strand, within HEPCGN_24790 at 3711.495 kb on - strand, within HEPCGN_24790 at 3711.495 kb on - strand, within HEPCGN_24790 at 3711.609 kb on - strand at 3711.670 kb on - strand at 3711.730 kb on - strand at 3711.730 kb on - strand at 3711.781 kb on + strand at 3711.924 kb on + strand, within nhaP2 at 3711.926 kb on + strand, within nhaP2 at 3711.926 kb on + strand, within nhaP2 at 3711.927 kb on - strand, within nhaP2 at 3711.927 kb on - strand, within nhaP2 at 3711.946 kb on - strand, within nhaP2 at 3711.946 kb on - strand, within nhaP2 at 3712.058 kb on + strand, within nhaP2 at 3712.058 kb on + strand, within nhaP2 at 3712.067 kb on + strand, within nhaP2 at 3712.068 kb on - strand, within nhaP2 at 3712.139 kb on - strand, within nhaP2 at 3712.209 kb on + strand, within nhaP2 at 3712.210 kb on - strand, within nhaP2 at 3712.370 kb on - strand, within nhaP2 at 3712.716 kb on + strand, within nhaP2 at 3712.781 kb on + strand, within nhaP2 at 3712.797 kb on + strand, within nhaP2 at 3712.845 kb on + strand, within nhaP2 at 3713.097 kb on - strand, within nhaP2 at 3713.199 kb on - strand, within nhaP2 at 3713.273 kb on + strand, within nhaP2 at 3713.274 kb on - strand, within nhaP2 at 3713.274 kb on - strand, within nhaP2 at 3713.274 kb on - strand, within nhaP2 at 3713.297 kb on - strand at 3713.297 kb on - strand at 3713.366 kb on - strand at 3713.395 kb on + strand at 3713.395 kb on + strand at 3713.396 kb on - strand at 3713.399 kb on + strand at 3713.399 kb on + strand at 3713.400 kb on - strand at 3713.400 kb on - strand at 3713.402 kb on + strand at 3713.404 kb on + strand at 3713.404 kb on + strand at 3713.429 kb on + strand at 3713.444 kb on - strand at 3713.496 kb on - strand at 3713.499 kb on + strand at 3713.511 kb on + strand at 3713.511 kb on + strand at 3713.511 kb on + strand at 3713.512 kb on - strand at 3713.512 kb on - strand at 3713.534 kb on + strand at 3713.567 kb on + strand at 3713.568 kb on - strand at 3713.615 kb on + strand at 3713.616 kb on - strand at 3713.741 kb on - strand, within ldcA at 3713.781 kb on - strand, within ldcA at 3713.888 kb on + strand, within ldcA at 3714.158 kb on + strand, within ldcA
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 3,710,798 - HEPCGN_24785 0.63 +0.3 3,710,800 - HEPCGN_24785 0.63 -0.3 3,710,839 - HEPCGN_24785 0.68 +1.4 3,710,839 - HEPCGN_24785 0.68 -0.5 3,710,839 - HEPCGN_24785 0.68 -0.3 3,710,893 + HEPCGN_24785 0.74 +0.3 3,710,893 + HEPCGN_24785 0.74 -0.9 3,710,917 - HEPCGN_24785 0.77 +0.2 3,710,972 + HEPCGN_24785 0.84 +0.0 3,711,097 - -0.2 3,711,114 + -0.3 3,711,114 + -0.9 3,711,119 - -0.1 3,711,119 - -1.5 3,711,129 + +0.1 3,711,130 - -1.4 3,711,204 - +1.6 3,711,204 - -2.0 3,711,205 + +0.1 3,711,206 - +0.3 3,711,206 - +0.2 3,711,206 - -0.6 3,711,206 - +0.3 3,711,210 - +0.1 3,711,210 - +1.3 3,711,259 - -0.1 3,711,259 - -0.7 3,711,267 + +0.4 3,711,267 + -0.3 3,711,282 - -0.1 3,711,282 - +0.7 3,711,293 - +0.8 3,711,293 - -1.2 3,711,296 - -1.0 3,711,305 - -0.1 3,711,353 + +0.2 3,711,401 - -0.2 3,711,412 + -0.3 3,711,488 + HEPCGN_24790 0.25 +0.7 3,711,495 - HEPCGN_24790 0.30 -0.6 3,711,495 - HEPCGN_24790 0.30 -0.6 3,711,609 - +0.6 3,711,670 - +0.0 3,711,730 - -0.5 3,711,730 - +0.6 3,711,781 + +0.4 3,711,924 + nhaP2 HEPCGN_24795 0.11 +0.4 3,711,926 + nhaP2 HEPCGN_24795 0.11 -0.5 3,711,926 + nhaP2 HEPCGN_24795 0.11 +0.2 3,711,927 - nhaP2 HEPCGN_24795 0.11 -0.7 3,711,927 - nhaP2 HEPCGN_24795 0.11 -1.7 3,711,946 - nhaP2 HEPCGN_24795 0.13 -0.4 3,711,946 - nhaP2 HEPCGN_24795 0.13 -0.4 3,712,058 + nhaP2 HEPCGN_24795 0.19 -0.9 3,712,058 + nhaP2 HEPCGN_24795 0.19 -0.5 3,712,067 + nhaP2 HEPCGN_24795 0.20 +0.6 3,712,068 - nhaP2 HEPCGN_24795 0.20 +2.0 3,712,139 - nhaP2 HEPCGN_24795 0.24 +0.9 3,712,209 + nhaP2 HEPCGN_24795 0.28 -0.3 3,712,210 - nhaP2 HEPCGN_24795 0.28 +0.1 3,712,370 - nhaP2 HEPCGN_24795 0.37 +0.0 3,712,716 + nhaP2 HEPCGN_24795 0.57 +0.0 3,712,781 + nhaP2 HEPCGN_24795 0.61 -0.4 3,712,797 + nhaP2 HEPCGN_24795 0.62 -0.3 3,712,845 + nhaP2 HEPCGN_24795 0.64 -0.4 3,713,097 - nhaP2 HEPCGN_24795 0.79 -0.3 3,713,199 - nhaP2 HEPCGN_24795 0.85 +0.4 3,713,273 + nhaP2 HEPCGN_24795 0.89 +0.6 3,713,274 - nhaP2 HEPCGN_24795 0.89 -0.5 3,713,274 - nhaP2 HEPCGN_24795 0.89 +0.0 3,713,274 - nhaP2 HEPCGN_24795 0.89 +0.0 3,713,297 - -1.7 3,713,297 - +0.7 3,713,366 - -0.1 3,713,395 + -0.8 3,713,395 + +1.3 3,713,396 - -0.5 3,713,399 + -0.6 3,713,399 + -0.6 3,713,400 - -0.2 3,713,400 - -0.3 3,713,402 + +0.6 3,713,404 + +0.6 3,713,404 + +0.3 3,713,429 + +0.6 3,713,444 - +1.6 3,713,496 - +1.2 3,713,499 + -0.1 3,713,511 + -0.3 3,713,511 + +0.5 3,713,511 + -0.5 3,713,512 - +1.3 3,713,512 - -0.4 3,713,534 + -0.5 3,713,567 + -0.5 3,713,568 - -0.5 3,713,615 + -0.3 3,713,616 - -0.6 3,713,741 - ldcA HEPCGN_24800 0.20 +0.1 3,713,781 - ldcA HEPCGN_24800 0.24 -0.7 3,713,888 + ldcA HEPCGN_24800 0.36 +0.3 3,714,158 + ldcA HEPCGN_24800 0.65 +1.6
Or see this region's nucleotide sequence