Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hscA and fdx are separated by 1 nucleotides fdx and iscX are separated by 11 nucleotides iscX and pepB are separated by 58 nucleotides pepB and sseB are separated by 118 nucleotides
HEPCGN_20780: hscA - Fe-S protein assembly chaperone HscA, at 2,895,132 to 2,896,982
hscA
HEPCGN_20785: fdx - ISC system 2Fe-2S type ferredoxin, at 2,896,984 to 2,897,319
fdx
HEPCGN_20790: iscX - Fe-S cluster assembly protein IscX, at 2,897,331 to 2,897,531
iscX
HEPCGN_20795: pepB - aminopeptidase PepB, at 2,897,590 to 2,898,873
pepB
HEPCGN_20800: sseB - enhanced serine sensitivity protein SseB, at 2,898,992 to 2,899,768
sseB
Position (kb)
2897
2898
2899 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2896.670 kb on + strand, within hscA at 2896.686 kb on + strand, within hscA at 2896.983 kb on + strand at 2897.046 kb on + strand, within fdx at 2897.047 kb on - strand, within fdx at 2897.049 kb on + strand, within fdx at 2897.295 kb on + strand at 2897.328 kb on - strand at 2897.328 kb on - strand at 2897.330 kb on + strand at 2897.330 kb on + strand at 2897.339 kb on + strand at 2897.339 kb on + strand at 2897.340 kb on - strand at 2897.340 kb on - strand at 2897.340 kb on - strand at 2897.340 kb on - strand at 2897.376 kb on + strand, within iscX at 2897.376 kb on + strand, within iscX at 2897.377 kb on - strand, within iscX at 2897.529 kb on + strand at 2897.586 kb on + strand at 2897.587 kb on - strand at 2897.611 kb on - strand at 2897.659 kb on + strand at 2897.660 kb on - strand at 2897.667 kb on + strand at 2897.667 kb on + strand at 2897.670 kb on + strand at 2897.671 kb on - strand at 2897.683 kb on - strand at 2897.683 kb on - strand at 2897.821 kb on + strand, within pepB at 2897.821 kb on + strand, within pepB at 2897.845 kb on - strand, within pepB at 2898.019 kb on + strand, within pepB at 2898.058 kb on + strand, within pepB at 2898.058 kb on + strand, within pepB at 2898.059 kb on - strand, within pepB at 2898.222 kb on + strand, within pepB at 2898.223 kb on - strand, within pepB at 2898.223 kb on - strand, within pepB at 2898.376 kb on - strand, within pepB at 2898.376 kb on - strand, within pepB at 2898.376 kb on - strand, within pepB at 2898.378 kb on + strand, within pepB at 2898.379 kb on - strand, within pepB at 2898.538 kb on + strand, within pepB at 2898.681 kb on + strand, within pepB at 2898.752 kb on - strand at 2898.855 kb on + strand at 2898.856 kb on - strand at 2898.881 kb on - strand at 2898.929 kb on - strand at 2898.991 kb on + strand at 2898.991 kb on + strand at 2898.992 kb on - strand at 2899.072 kb on + strand, within sseB at 2899.395 kb on + strand, within sseB at 2899.422 kb on + strand, within sseB at 2899.483 kb on + strand, within sseB at 2899.484 kb on - strand, within sseB at 2899.484 kb on - strand, within sseB at 2899.507 kb on + strand, within sseB at 2899.544 kb on - strand, within sseB at 2899.553 kb on - strand, within sseB at 2899.608 kb on + strand, within sseB at 2899.609 kb on - strand, within sseB at 2899.618 kb on + strand, within sseB at 2899.624 kb on - strand, within sseB at 2899.624 kb on - strand, within sseB at 2899.645 kb on + strand, within sseB at 2899.700 kb on - strand at 2899.713 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 2,896,670 + hscA HEPCGN_20780 0.83 +0.8 2,896,686 + hscA HEPCGN_20780 0.84 -1.5 2,896,983 + -0.2 2,897,046 + fdx HEPCGN_20785 0.18 +0.8 2,897,047 - fdx HEPCGN_20785 0.19 +0.8 2,897,049 + fdx HEPCGN_20785 0.19 +1.8 2,897,295 + -2.2 2,897,328 - -0.4 2,897,328 - -1.0 2,897,330 + +0.8 2,897,330 + -0.5 2,897,339 + -1.4 2,897,339 + +0.2 2,897,340 - +0.4 2,897,340 - -0.1 2,897,340 - -0.5 2,897,340 - -0.2 2,897,376 + iscX HEPCGN_20790 0.22 +0.7 2,897,376 + iscX HEPCGN_20790 0.22 +0.6 2,897,377 - iscX HEPCGN_20790 0.23 -0.2 2,897,529 + +0.2 2,897,586 + +0.7 2,897,587 - -0.0 2,897,611 - -0.0 2,897,659 + -0.5 2,897,660 - +0.0 2,897,667 + +0.7 2,897,667 + -0.8 2,897,670 + -1.0 2,897,671 - +0.5 2,897,683 - +0.8 2,897,683 - -0.3 2,897,821 + pepB HEPCGN_20795 0.18 -0.9 2,897,821 + pepB HEPCGN_20795 0.18 +0.4 2,897,845 - pepB HEPCGN_20795 0.20 -0.2 2,898,019 + pepB HEPCGN_20795 0.33 +0.3 2,898,058 + pepB HEPCGN_20795 0.36 -0.0 2,898,058 + pepB HEPCGN_20795 0.36 +0.2 2,898,059 - pepB HEPCGN_20795 0.37 +0.5 2,898,222 + pepB HEPCGN_20795 0.49 +0.5 2,898,223 - pepB HEPCGN_20795 0.49 +0.1 2,898,223 - pepB HEPCGN_20795 0.49 +0.6 2,898,376 - pepB HEPCGN_20795 0.61 -0.2 2,898,376 - pepB HEPCGN_20795 0.61 -1.2 2,898,376 - pepB HEPCGN_20795 0.61 +0.6 2,898,378 + pepB HEPCGN_20795 0.61 +0.6 2,898,379 - pepB HEPCGN_20795 0.61 +0.1 2,898,538 + pepB HEPCGN_20795 0.74 +0.5 2,898,681 + pepB HEPCGN_20795 0.85 +0.9 2,898,752 - +0.4 2,898,855 + +1.1 2,898,856 - -1.4 2,898,881 - -2.0 2,898,929 - +0.7 2,898,991 + +0.3 2,898,991 + +0.4 2,898,992 - +0.3 2,899,072 + sseB HEPCGN_20800 0.10 -0.6 2,899,395 + sseB HEPCGN_20800 0.52 -0.9 2,899,422 + sseB HEPCGN_20800 0.55 +0.2 2,899,483 + sseB HEPCGN_20800 0.63 -0.2 2,899,484 - sseB HEPCGN_20800 0.63 -1.0 2,899,484 - sseB HEPCGN_20800 0.63 +0.3 2,899,507 + sseB HEPCGN_20800 0.66 +0.1 2,899,544 - sseB HEPCGN_20800 0.71 -0.3 2,899,553 - sseB HEPCGN_20800 0.72 -0.4 2,899,608 + sseB HEPCGN_20800 0.79 +0.1 2,899,609 - sseB HEPCGN_20800 0.79 -0.2 2,899,618 + sseB HEPCGN_20800 0.81 -0.7 2,899,624 - sseB HEPCGN_20800 0.81 +0.0 2,899,624 - sseB HEPCGN_20800 0.81 -1.4 2,899,645 + sseB HEPCGN_20800 0.84 +0.2 2,899,700 - -0.3 2,899,713 - +0.6
Or see this region's nucleotide sequence