Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysN and cysC overlap by 1 nucleotides cysC and ygbE are separated by 49 nucleotides ygbE and ftsB are separated by 193 nucleotides ftsB and ispD are separated by 18 nucleotides
HEPCGN_19625: cysN - sulfate adenylyltransferase subunit CysN, at 2,683,430 to 2,684,857
cysN
HEPCGN_19630: cysC - Adenylyl-sulfate kinase, at 2,684,857 to 2,685,462
cysC
HEPCGN_19635: ygbE - DUF3561 domain-containing protein, at 2,685,512 to 2,685,835
ygbE
HEPCGN_19640: ftsB - cell division protein FtsB, at 2,686,029 to 2,686,340
ftsB
HEPCGN_19645: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 2,686,359 to 2,687,069
ispD
Position (kb)
2685
2686 Strain fitness (log2 ratio)
-2
-1
0
1 at 2684.557 kb on + strand, within cysN at 2684.564 kb on - strand, within cysN at 2684.616 kb on + strand, within cysN at 2684.616 kb on + strand, within cysN at 2684.617 kb on - strand, within cysN at 2684.647 kb on - strand, within cysN at 2684.704 kb on + strand, within cysN at 2684.716 kb on - strand at 2684.716 kb on - strand at 2684.852 kb on - strand at 2684.856 kb on - strand at 2684.901 kb on - strand at 2685.011 kb on + strand, within cysC at 2685.012 kb on - strand, within cysC at 2685.015 kb on + strand, within cysC at 2685.016 kb on - strand, within cysC at 2685.031 kb on - strand, within cysC at 2685.034 kb on + strand, within cysC at 2685.034 kb on + strand, within cysC at 2685.034 kb on + strand, within cysC at 2685.035 kb on - strand, within cysC at 2685.068 kb on + strand, within cysC at 2685.069 kb on - strand, within cysC at 2685.087 kb on + strand, within cysC at 2685.178 kb on - strand, within cysC at 2685.222 kb on - strand, within cysC at 2685.296 kb on + strand, within cysC at 2685.297 kb on - strand, within cysC at 2685.298 kb on + strand, within cysC at 2685.299 kb on - strand, within cysC at 2685.300 kb on + strand, within cysC at 2685.300 kb on + strand, within cysC at 2685.341 kb on + strand, within cysC at 2685.422 kb on + strand at 2685.447 kb on + strand at 2685.448 kb on - strand at 2685.512 kb on - strand at 2685.520 kb on + strand at 2685.521 kb on - strand at 2685.526 kb on + strand at 2685.532 kb on + strand at 2685.533 kb on - strand at 2685.533 kb on - strand at 2685.544 kb on + strand at 2685.544 kb on + strand at 2685.618 kb on + strand, within ygbE at 2685.618 kb on + strand, within ygbE at 2685.647 kb on + strand, within ygbE at 2685.648 kb on - strand, within ygbE at 2685.673 kb on + strand, within ygbE at 2685.759 kb on + strand, within ygbE at 2685.759 kb on + strand, within ygbE at 2685.760 kb on - strand, within ygbE at 2685.764 kb on + strand, within ygbE at 2685.765 kb on - strand, within ygbE at 2685.765 kb on - strand, within ygbE at 2685.799 kb on + strand, within ygbE at 2685.799 kb on + strand, within ygbE at 2685.799 kb on + strand, within ygbE at 2685.800 kb on - strand, within ygbE at 2685.800 kb on - strand, within ygbE at 2685.817 kb on + strand at 2685.863 kb on + strand at 2685.879 kb on + strand at 2685.880 kb on - strand at 2685.880 kb on - strand at 2685.882 kb on + strand at 2685.882 kb on + strand at 2685.891 kb on + strand at 2685.892 kb on - strand at 2685.911 kb on + strand at 2685.911 kb on + strand at 2685.913 kb on + strand at 2685.913 kb on + strand at 2685.913 kb on + strand at 2685.913 kb on + strand at 2685.914 kb on - strand at 2685.914 kb on - strand at 2685.918 kb on - strand at 2685.936 kb on + strand at 2685.977 kb on + strand at 2685.977 kb on + strand at 2685.977 kb on + strand at 2685.978 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 2,684,557 + cysN HEPCGN_19625 0.79 -0.4 2,684,564 - cysN HEPCGN_19625 0.79 -0.9 2,684,616 + cysN HEPCGN_19625 0.83 +0.8 2,684,616 + cysN HEPCGN_19625 0.83 +1.4 2,684,617 - cysN HEPCGN_19625 0.83 +1.3 2,684,647 - cysN HEPCGN_19625 0.85 -0.4 2,684,704 + cysN HEPCGN_19625 0.89 -1.2 2,684,716 - -0.2 2,684,716 - -1.2 2,684,852 - -0.6 2,684,856 - -0.6 2,684,901 - -0.2 2,685,011 + cysC HEPCGN_19630 0.25 +0.9 2,685,012 - cysC HEPCGN_19630 0.26 +1.3 2,685,015 + cysC HEPCGN_19630 0.26 +0.4 2,685,016 - cysC HEPCGN_19630 0.26 -0.1 2,685,031 - cysC HEPCGN_19630 0.29 -0.2 2,685,034 + cysC HEPCGN_19630 0.29 +1.0 2,685,034 + cysC HEPCGN_19630 0.29 -0.0 2,685,034 + cysC HEPCGN_19630 0.29 -1.1 2,685,035 - cysC HEPCGN_19630 0.29 -0.1 2,685,068 + cysC HEPCGN_19630 0.35 -0.3 2,685,069 - cysC HEPCGN_19630 0.35 +0.7 2,685,087 + cysC HEPCGN_19630 0.38 -0.2 2,685,178 - cysC HEPCGN_19630 0.53 -0.2 2,685,222 - cysC HEPCGN_19630 0.60 -0.5 2,685,296 + cysC HEPCGN_19630 0.72 +1.5 2,685,297 - cysC HEPCGN_19630 0.73 +1.5 2,685,298 + cysC HEPCGN_19630 0.73 +1.4 2,685,299 - cysC HEPCGN_19630 0.73 -0.0 2,685,300 + cysC HEPCGN_19630 0.73 -0.2 2,685,300 + cysC HEPCGN_19630 0.73 -0.4 2,685,341 + cysC HEPCGN_19630 0.80 -0.2 2,685,422 + +1.0 2,685,447 + -0.3 2,685,448 - -1.2 2,685,512 - -1.4 2,685,520 + -0.8 2,685,521 - -0.7 2,685,526 + -0.0 2,685,532 + -0.0 2,685,533 - -0.8 2,685,533 - -0.7 2,685,544 + -0.0 2,685,544 + -1.1 2,685,618 + ygbE HEPCGN_19635 0.33 +0.3 2,685,618 + ygbE HEPCGN_19635 0.33 -2.1 2,685,647 + ygbE HEPCGN_19635 0.42 -0.1 2,685,648 - ygbE HEPCGN_19635 0.42 +0.6 2,685,673 + ygbE HEPCGN_19635 0.50 -2.3 2,685,759 + ygbE HEPCGN_19635 0.76 +0.3 2,685,759 + ygbE HEPCGN_19635 0.76 -0.5 2,685,760 - ygbE HEPCGN_19635 0.77 -1.2 2,685,764 + ygbE HEPCGN_19635 0.78 -1.6 2,685,765 - ygbE HEPCGN_19635 0.78 +0.0 2,685,765 - ygbE HEPCGN_19635 0.78 +0.2 2,685,799 + ygbE HEPCGN_19635 0.89 -0.0 2,685,799 + ygbE HEPCGN_19635 0.89 -2.1 2,685,799 + ygbE HEPCGN_19635 0.89 -0.2 2,685,800 - ygbE HEPCGN_19635 0.89 -0.6 2,685,800 - ygbE HEPCGN_19635 0.89 -0.5 2,685,817 + -0.1 2,685,863 + +0.4 2,685,879 + -1.2 2,685,880 - +0.3 2,685,880 - -0.4 2,685,882 + -0.7 2,685,882 + -0.3 2,685,891 + -0.3 2,685,892 - -1.2 2,685,911 + -0.6 2,685,911 + -0.8 2,685,913 + +0.4 2,685,913 + -0.2 2,685,913 + +0.6 2,685,913 + +0.2 2,685,914 - +0.1 2,685,914 - -1.2 2,685,918 - -0.5 2,685,936 + -0.5 2,685,977 + -0.2 2,685,977 + -0.3 2,685,977 + -0.1 2,685,978 - +0.8
Or see this region's nucleotide sequence