Strain Fitness in Escherichia coli ECOR38 around HEPCGN_18120

Experiment: RB23

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntneuB and neuA overlap by 1 nucleotidesneuA and neuC are separated by 62 nucleotides HEPCGN_18115: neuB - N-acylneuraminate-9-phosphate synthase, at 2,371,553 to 2,372,593 neuB HEPCGN_18120: neuA - N-acylneuraminate cytidylyltransferase, at 2,372,593 to 2,373,849 neuA HEPCGN_18125: neuC - UDP-N-acetylglucosamine 2-epimerase, at 2,373,912 to 2,375,021 neuC Position (kb) 2372 2373 2374Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2371.784 kb on + strand, within neuBat 2372.509 kb on + strandat 2372.592 kb on - strandat 2372.692 kb on + strandat 2372.782 kb on + strand, within neuAat 2372.782 kb on + strand, within neuAat 2372.869 kb on + strand, within neuAat 2373.996 kb on - strandat 2373.998 kb on + strandat 2374.158 kb on + strand, within neuCat 2374.241 kb on + strand, within neuCat 2374.358 kb on + strand, within neuCat 2374.362 kb on - strand, within neuCat 2374.409 kb on + strand, within neuCat 2374.520 kb on - strand, within neuCat 2374.597 kb on + strand, within neuC

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Per-strain Table

Position Strand Gene LocusTag Fraction RB23
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2,371,784 + neuB HEPCGN_18115 0.22 -4.1
2,372,509 + -3.5
2,372,592 - +0.4
2,372,692 + -4.2
2,372,782 + neuA HEPCGN_18120 0.15 -1.5
2,372,782 + neuA HEPCGN_18120 0.15 -1.6
2,372,869 + neuA HEPCGN_18120 0.22 -4.3
2,373,996 - -0.4
2,373,998 + -4.7
2,374,158 + neuC HEPCGN_18125 0.22 -3.1
2,374,241 + neuC HEPCGN_18125 0.30 -3.1
2,374,358 + neuC HEPCGN_18125 0.40 -3.4
2,374,362 - neuC HEPCGN_18125 0.41 -2.1
2,374,409 + neuC HEPCGN_18125 0.45 -4.6
2,374,520 - neuC HEPCGN_18125 0.55 -3.6
2,374,597 + neuC HEPCGN_18125 0.62 -1.2

Or see this region's nucleotide sequence