Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt greB and ompR_G63V are separated by 227 nucleotides ompR_G63V and envZ overlap by 4 nucleotides envZ and yiaG are separated by 31 nucleotides yiaG and HEPCGN_15860 are separated by 58 nucleotides HEPCGN_15860 and pckA are separated by 114 nucleotides
HEPCGN_15840: greB - transcription elongation factor GreB, at 1,941,016 to 1,941,492
greB
HEPCGN_15845: ompR_G63V - two-component system response regulator OmpR, at 1,941,720 to 1,942,439
_G63V
HEPCGN_15850: envZ - two-component system sensor histidine kinase EnvZ, at 1,942,436 to 1,943,788
envZ
HEPCGN_15855: yiaG - transcriptional regulator, at 1,943,820 to 1,944,116
yiaG
HEPCGN_15860: HEPCGN_15860 - toxin HigB-2, at 1,944,175 to 1,944,492
_15860
HEPCGN_15865: pckA - phosphoenolpyruvate carboxykinase (ATP), at 1,944,607 to 1,946,229
pckA
Position (kb)
1942
1943
1944 Strain fitness (log2 ratio)
-1
0
1
2 at 1941.440 kb on - strand, within greB at 1941.535 kb on + strand at 1941.538 kb on - strand at 1941.549 kb on - strand at 1941.578 kb on + strand at 1941.591 kb on + strand at 1941.592 kb on - strand at 1941.592 kb on - strand at 1941.592 kb on - strand at 1941.604 kb on - strand at 1941.604 kb on - strand at 1941.663 kb on - strand at 1941.713 kb on - strand at 1941.732 kb on + strand at 1941.758 kb on + strand at 1941.759 kb on - strand at 1941.786 kb on + strand at 1941.872 kb on + strand, within ompR_G63V at 1941.872 kb on + strand, within ompR_G63V at 1941.873 kb on - strand, within ompR_G63V at 1941.873 kb on - strand, within ompR_G63V at 1941.877 kb on + strand, within ompR_G63V at 1941.892 kb on + strand, within ompR_G63V at 1941.892 kb on + strand, within ompR_G63V at 1941.893 kb on - strand, within ompR_G63V at 1941.959 kb on + strand, within ompR_G63V at 1941.960 kb on - strand, within ompR_G63V at 1941.960 kb on - strand, within ompR_G63V at 1942.299 kb on + strand, within ompR_G63V at 1942.408 kb on - strand at 1942.417 kb on + strand at 1942.430 kb on + strand at 1942.430 kb on + strand at 1942.526 kb on + strand at 1942.527 kb on - strand at 1942.573 kb on + strand, within envZ at 1942.574 kb on - strand, within envZ at 1942.574 kb on - strand, within envZ at 1942.576 kb on + strand, within envZ at 1942.576 kb on + strand, within envZ at 1942.601 kb on - strand, within envZ at 1942.601 kb on - strand, within envZ at 1942.601 kb on - strand, within envZ at 1942.601 kb on - strand, within envZ at 1942.676 kb on + strand, within envZ at 1942.676 kb on + strand, within envZ at 1942.676 kb on + strand, within envZ at 1942.701 kb on - strand, within envZ at 1942.924 kb on + strand, within envZ at 1942.924 kb on + strand, within envZ at 1942.925 kb on - strand, within envZ at 1942.937 kb on - strand, within envZ at 1943.036 kb on - strand, within envZ at 1943.057 kb on + strand, within envZ at 1943.116 kb on + strand, within envZ at 1943.117 kb on - strand, within envZ at 1943.174 kb on - strand, within envZ at 1943.229 kb on - strand, within envZ at 1943.249 kb on - strand, within envZ at 1943.295 kb on - strand, within envZ at 1943.379 kb on - strand, within envZ at 1943.547 kb on + strand, within envZ at 1943.584 kb on + strand, within envZ at 1943.584 kb on + strand, within envZ at 1943.584 kb on + strand, within envZ at 1943.584 kb on + strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.585 kb on - strand, within envZ at 1943.787 kb on - strand at 1943.935 kb on + strand, within yiaG at 1943.935 kb on + strand, within yiaG at 1943.974 kb on - strand, within yiaG at 1943.974 kb on - strand, within yiaG at 1943.974 kb on - strand, within yiaG at 1944.000 kb on + strand, within yiaG at 1944.020 kb on + strand, within yiaG at 1944.196 kb on + strand at 1944.227 kb on - strand, within HEPCGN_15860 at 1944.286 kb on + strand, within HEPCGN_15860 at 1944.286 kb on + strand, within HEPCGN_15860 at 1944.296 kb on + strand, within HEPCGN_15860 at 1944.297 kb on - strand, within HEPCGN_15860 at 1944.376 kb on - strand, within HEPCGN_15860 at 1944.406 kb on + strand, within HEPCGN_15860 at 1944.406 kb on + strand, within HEPCGN_15860 at 1944.406 kb on + strand, within HEPCGN_15860 at 1944.407 kb on - strand, within HEPCGN_15860 at 1944.439 kb on + strand, within HEPCGN_15860 at 1944.446 kb on - strand, within HEPCGN_15860 at 1944.446 kb on - strand, within HEPCGN_15860 at 1944.492 kb on + strand at 1944.493 kb on - strand at 1944.493 kb on - strand at 1944.510 kb on - strand at 1944.516 kb on - strand at 1944.519 kb on + strand at 1944.520 kb on - strand at 1944.538 kb on + strand at 1944.553 kb on - strand at 1944.553 kb on - strand at 1944.609 kb on - strand at 1944.609 kb on - strand at 1944.629 kb on - strand at 1944.659 kb on + strand at 1944.660 kb on - strand at 1944.664 kb on - strand at 1944.731 kb on + strand at 1944.731 kb on + strand at 1944.731 kb on + strand at 1944.732 kb on - strand at 1944.732 kb on - strand at 1944.732 kb on - strand at 1944.732 kb on - strand at 1944.732 kb on - strand at 1944.763 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 1,941,440 - greB HEPCGN_15840 0.89 -1.0 1,941,535 + +0.6 1,941,538 - -0.5 1,941,549 - -0.5 1,941,578 + +0.7 1,941,591 + -0.9 1,941,592 - +0.3 1,941,592 - +0.1 1,941,592 - +0.7 1,941,604 - +1.5 1,941,604 - +0.4 1,941,663 - +0.7 1,941,713 - -0.1 1,941,732 + +0.0 1,941,758 + +0.7 1,941,759 - -0.8 1,941,786 + -1.7 1,941,872 + ompR_G63V HEPCGN_15845 0.21 +0.1 1,941,872 + ompR_G63V HEPCGN_15845 0.21 +0.7 1,941,873 - ompR_G63V HEPCGN_15845 0.21 -0.3 1,941,873 - ompR_G63V HEPCGN_15845 0.21 +0.2 1,941,877 + ompR_G63V HEPCGN_15845 0.22 -0.2 1,941,892 + ompR_G63V HEPCGN_15845 0.24 -1.3 1,941,892 + ompR_G63V HEPCGN_15845 0.24 -0.8 1,941,893 - ompR_G63V HEPCGN_15845 0.24 +0.2 1,941,959 + ompR_G63V HEPCGN_15845 0.33 -0.6 1,941,960 - ompR_G63V HEPCGN_15845 0.33 -0.5 1,941,960 - ompR_G63V HEPCGN_15845 0.33 +1.7 1,942,299 + ompR_G63V HEPCGN_15845 0.80 -1.3 1,942,408 - +0.2 1,942,417 + -0.1 1,942,430 + +0.8 1,942,430 + -0.1 1,942,526 + +0.4 1,942,527 - -0.6 1,942,573 + envZ HEPCGN_15850 0.10 -0.2 1,942,574 - envZ HEPCGN_15850 0.10 +0.5 1,942,574 - envZ HEPCGN_15850 0.10 +0.2 1,942,576 + envZ HEPCGN_15850 0.10 -0.2 1,942,576 + envZ HEPCGN_15850 0.10 +1.7 1,942,601 - envZ HEPCGN_15850 0.12 +0.1 1,942,601 - envZ HEPCGN_15850 0.12 -0.2 1,942,601 - envZ HEPCGN_15850 0.12 +0.1 1,942,601 - envZ HEPCGN_15850 0.12 -0.5 1,942,676 + envZ HEPCGN_15850 0.18 +1.1 1,942,676 + envZ HEPCGN_15850 0.18 +1.2 1,942,676 + envZ HEPCGN_15850 0.18 +0.8 1,942,701 - envZ HEPCGN_15850 0.20 +0.9 1,942,924 + envZ HEPCGN_15850 0.36 +0.1 1,942,924 + envZ HEPCGN_15850 0.36 +0.6 1,942,925 - envZ HEPCGN_15850 0.36 -0.2 1,942,937 - envZ HEPCGN_15850 0.37 +0.5 1,943,036 - envZ HEPCGN_15850 0.44 +0.5 1,943,057 + envZ HEPCGN_15850 0.46 +0.6 1,943,116 + envZ HEPCGN_15850 0.50 +0.5 1,943,117 - envZ HEPCGN_15850 0.50 +0.5 1,943,174 - envZ HEPCGN_15850 0.55 -1.6 1,943,229 - envZ HEPCGN_15850 0.59 +1.7 1,943,249 - envZ HEPCGN_15850 0.60 -0.3 1,943,295 - envZ HEPCGN_15850 0.63 -0.3 1,943,379 - envZ HEPCGN_15850 0.70 +0.7 1,943,547 + envZ HEPCGN_15850 0.82 +1.2 1,943,584 + envZ HEPCGN_15850 0.85 -0.0 1,943,584 + envZ HEPCGN_15850 0.85 -0.6 1,943,584 + envZ HEPCGN_15850 0.85 +1.0 1,943,584 + envZ HEPCGN_15850 0.85 +0.8 1,943,585 - envZ HEPCGN_15850 0.85 -0.1 1,943,585 - envZ HEPCGN_15850 0.85 +0.8 1,943,585 - envZ HEPCGN_15850 0.85 +2.6 1,943,585 - envZ HEPCGN_15850 0.85 -1.0 1,943,585 - envZ HEPCGN_15850 0.85 +0.3 1,943,585 - envZ HEPCGN_15850 0.85 +0.5 1,943,787 - +0.1 1,943,935 + yiaG HEPCGN_15855 0.39 +1.8 1,943,935 + yiaG HEPCGN_15855 0.39 +0.1 1,943,974 - yiaG HEPCGN_15855 0.52 +0.4 1,943,974 - yiaG HEPCGN_15855 0.52 -0.1 1,943,974 - yiaG HEPCGN_15855 0.52 +0.1 1,944,000 + yiaG HEPCGN_15855 0.61 -0.0 1,944,020 + yiaG HEPCGN_15855 0.67 -1.7 1,944,196 + -0.2 1,944,227 - HEPCGN_15860 0.16 -0.2 1,944,286 + HEPCGN_15860 0.35 +0.2 1,944,286 + HEPCGN_15860 0.35 -0.6 1,944,296 + HEPCGN_15860 0.38 -0.4 1,944,297 - HEPCGN_15860 0.38 +0.6 1,944,376 - HEPCGN_15860 0.63 -0.1 1,944,406 + HEPCGN_15860 0.73 +0.4 1,944,406 + HEPCGN_15860 0.73 +0.4 1,944,406 + HEPCGN_15860 0.73 +0.4 1,944,407 - HEPCGN_15860 0.73 -0.0 1,944,439 + HEPCGN_15860 0.83 -1.7 1,944,446 - HEPCGN_15860 0.85 -0.2 1,944,446 - HEPCGN_15860 0.85 +0.1 1,944,492 + -0.1 1,944,493 - -1.3 1,944,493 - +0.5 1,944,510 - -0.0 1,944,516 - -0.3 1,944,519 + -0.5 1,944,520 - -0.5 1,944,538 + -0.2 1,944,553 - +0.2 1,944,553 - -0.0 1,944,609 - -0.2 1,944,609 - +0.5 1,944,629 - -0.7 1,944,659 + -0.1 1,944,660 - +0.3 1,944,664 - -0.4 1,944,731 + +0.1 1,944,731 + +0.2 1,944,731 + -0.2 1,944,732 - +0.3 1,944,732 - -0.0 1,944,732 - +0.0 1,944,732 - +0.3 1,944,732 - -1.4 1,944,763 + +0.3
Or see this region's nucleotide sequence