Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnpA and rpmH are separated by 49 nucleotides rpmH and ysdD are separated by 229 nucleotides ysdD and dnaA are separated by 296 nucleotides dnaA and dnaN are separated by 4 nucleotides
HEPCGN_13690: rnpA - ribonuclease P protein component, at 1,490,366 to 1,490,692
rnpA
HEPCGN_13695: rpmH - 50S ribosomal protein L34, at 1,490,742 to 1,490,882
rpmH
HEPCGN_13700: ysdD - Protein YsdD, at 1,491,112 to 1,491,192
ysdD
HEPCGN_13705: dnaA - chromosomal replication initiator protein DnaA, at 1,491,489 to 1,492,892
dnaA
HEPCGN_13715: dnaN - DNA polymerase III subunit beta, at 1,492,897 to 1,493,997
dnaN
Position (kb) 1491
1492
1493 Strain fitness (log2 ratio) -2
-1
0
1 at 1491.029 kb on + strand at 1491.030 kb on - strand at 1491.113 kb on + strand at 1491.114 kb on - strand at 1491.159 kb on + strand, within ysdD at 1491.160 kb on - strand, within ysdD at 1491.185 kb on - strand at 1491.185 kb on - strand at 1491.206 kb on - strand at 1491.214 kb on + strand at 1491.215 kb on - strand at 1491.373 kb on + strand at 1491.424 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 1,491,029 + -2.2 1,491,030 - -0.3 1,491,113 + -0.9 1,491,114 - +1.0 1,491,159 + ysdD HEPCGN_13700 0.58 -0.9 1,491,160 - ysdD HEPCGN_13700 0.59 +0.6 1,491,185 - -0.0 1,491,185 - -0.6 1,491,206 - -0.2 1,491,214 + -0.2 1,491,215 - -0.5 1,491,373 + +1.3 1,491,424 + -0.2
Or see this region's nucleotide sequence